pRORB-donor
(Plasmid
#176354)
-
PurposeCRISPR donor plasmid to create GFP fusion proteins
-
Depositing Labs
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 176354 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepUC19
-
Vector typeCRISPR
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameRORB
-
SpeciesH. sapiens (human)
-
Entrez GeneRORB (a.k.a. NR1F2, ROR-BETA, RZR-BETA, RZRB, bA133M9.1)
-
Tag
/ Fusion Protein
- GFP (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Cloning source: WTC11 cells. Our lab normally uses Top10 cells for transformation.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pRORB-donor was a gift from Barbara Stranger & Kevin White (Addgene plasmid # 176354 ; http://n2t.net/addgene:176354 ; RRID:Addgene_176354)