Addgene

Showing: 8721 - 8740 of 52590 results

Results per page:

Sort results by:

YIp211-ADH-myc-E3(1)-CUbo-VSV

Plasmid

#212736

Purpose

Consitutive expression of myc-E3(1)-CUbo-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(1)

Use

Integrative vector

Tags

CUbo-VSV and myc

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
YIp128-ADH-myc-E3(1)-CUbo-VSV

Plasmid

#212735

Purpose

Consitutive expression of myc-E3(1)-CUbo-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(1)

Use

Integrative vector

Tags

CUbo-VSV and myc

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-FRT-TO-NUbo-E3(48)-FLAG

Plasmid

#212802

Purpose

Constitutive/Doxycycline-inducible expression of NUbo-E3(48)-FLAG in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(48)

Use

Tags

FLAG and NUbo

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-FRT-TO-Myc-E3(1)-CUbo

Plasmid

#212799

Purpose

Constitutive/Doxycycline-inducible expression of Myc-E3(1)-CUbo in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(1)

Use

Tags

CUbo and Myc

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-FRT-TO-His6-NUbo-H2B-His8

Plasmid

#212811

Purpose

Constitutive or doxycycline-inducible expression of His6-NUbo-H2B-His8 in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

H2B

Use

Tags

6xHis-NUbo and 8xHis

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-FRT-TO-His8-H2B-CUbo(K48R)

Plasmid

#212813

Purpose

Constitutive or doxycycline-inducible expression of His8-H2B-CUbo(K48R) in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

H2B

Use

Tags

8xHis and CUbo(K48R)

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
OR5-consensus

Plasmid

#208062

Purpose

Consensus OR5 engineered from human OR5 family members

Depositor

Hiroaki Matsunami

Article

Ikegami et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

OR5-consensus

Use

Tags

Rhodopsin tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
OR51-consensus

Plasmid

#208065

Purpose

Consensus OR51 engineered from human OR51 family members

Depositor

Hiroaki Matsunami

Article

Ikegami et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

OR51-consensus

Use

Tags

Rhodopsin tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
OR6-consensus

Plasmid

#208063

Purpose

Consensus OR6 engineered from human OR6 family members

Depositor

Hiroaki Matsunami

Article

Ikegami et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

OR6-consensus

Use

Tags

Rhodopsin tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
OR8-consensus

Plasmid

#208064

Purpose

Consensus OR8 engineered from human OR8 family members

Depositor

Hiroaki Matsunami

Article

Ikegami et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

OR8-consensus

Use

Tags

Rhodopsin tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
OR1-consensus

Plasmid

#208059

Purpose

Consensus OR1 engineered from human OR1 family members

Depositor

Hiroaki Matsunami

Article

Ikegami et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

OR1-consensus

Use

Tags

Rhodopsin tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
OR2-consensus

Plasmid

#208060

Purpose

Consensus OR2 engineered from human OR2 family members

Depositor

Hiroaki Matsunami

Article

Ikegami et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

OR2-consensus

Use

Tags

Rhodopsin tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
OR10-consensus

Plasmid

#208058

Purpose

Consensus OR10 engineered from human OR10 family members

Depositor

Hiroaki Matsunami

Article

Ikegami et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

OR10-consensus

Use

Tags

Rhodopsin tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
OR4-consensus

Plasmid

#208061

Purpose

Consensus OR4 engineered from human OR4 family members

Depositor

Hiroaki Matsunami

Article

Ikegami et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

OR4-consensus

Use

Tags

Rhodopsin tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
OR52-consensus

Plasmid

#208080

Purpose

Consensus OR52 engineered from human OR52 family members

Depositor

Hiroaki Matsunami

Article

Ikegami et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

OR52-consensus

Use

Tags

Rhodopsin tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4-Xpress-GBF1(E794K)

Plasmid

#206418

Purpose

Mammalian expression of hGBF1(E794K)

Depositor

Kazuhisa Nakayama

Article

Kawamoto et al Traffic. 2002 Jul;3(7):483-95. doi: 10.1

Insert

GBF1 (GBF1 Human)

Use

Tags

Expression

Mammalian

Mutation

E794K

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-28a(+)-6xHis-TEV-ORF9c

Plasmid

#201023

Purpose

Bacterial expression of codon optimized SARS-CoV-2 ORF9c tagged with an N-terminus His tag followed by a TEV cleavage site.

Depositor

Sharon Rozovsky

Article

Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

Insert

open reading frame 9c

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLA2ScIfmTcIfm

Plasmid

#193792

Purpose

Cassette 1: Expresses frame-shifted CI under PLLacO-1 promoter, Cassette 2: Expresses frame-shifted CI under PLTetO-1 promoter, ColE1 origin of replication, Ampicillin selection

Depositor

Sangram Bagh

Article

Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

Insert

Two copies of frame-shifted CI in opposite orientation, controlled by separate promoters and terminators

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PLLacO-1 and PLTetO-1

Available since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRA1SEGFPTcIfm

Plasmid

#193777

Purpose

Cassette 1: Expresses EGFP under the control of PR promoter, Cassette 2: Expresses frame-shifted CI under the control of PLTetO-1 promoter, pUC origin of replication, Ampicillin selection

Depositor

Sangram Bagh

Article

Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

Insert

EGFP and frame-shifted CI in opposite orientation, controlled by separate promoters and terminators

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PR promoter and PLTetO-1

Available since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CMV-Hsp1-Hsp2Cas9-KY-P2A-puro

Plasmid

#192133

Purpose

Expresses highly specific Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene

Depositor

Yongming Wang

Article

Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

Insert

Hsp1-Hsp2Cas9-KY

Use

AAV

Tags

Expression

Mammalian

Mutation

Hsp1-Hsp2Cas9 (K390A, Y446A)

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge