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Plasmid#137130PurposeIntersectional viral expression of BFP in cells expressing Cre AND NOT FlpDepositorHas ServiceAAV8InsertCon/Foff 2.0-BFP
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationn/aPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Fon/Von GCaMP 6m
Plasmid#137165PurposeIntersectional viral expression of GCaMP6M in cells expressing Cre AND Flp AND VcreDepositorInsert3x-GCaMP6M
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationRemoved RSET tagPromoterEf1aAvailable sinceNov. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Coff/Fon-sRGECO
Plasmid#137128PurposeIntersectional viral expression of sRGECO in cells expressing Flp AND NOT CreDepositorHas ServiceAAV8InsertCoff/Fon-sRGECO
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationE217DPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS0045
Plasmid#131124PurposeMammalian Target-ACE expression vectorDepositorInsertTadA-linker(32aa)-TadA*(7.10)-linker(32aa)-nCas9-PmCDA1-UGI
UseCRISPRTagsPmCDA1, TadA, and UGIExpressionMammalianMutationA262T, R324L, S409I, E480K, E543D, M694I, E1219V …PromoterCMV promoterAvailable sinceSept. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/FGFR2-S252W(Apert) myc
Plasmid#33249DepositorInsertFGFR2 (Fgfr2 Mouse)
UseTagsHis and MycExpressionMammalianMutationMutation S252W in FGFR2 found in Apert syndrome. …PromoterCMVAvailable sinceDec. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCD5-H5_Vietnam_1203/04_Y161A_sfGFP_ST
Plasmid#182547PurposeExpresses a sfGFP fused H5 protein of the prototype A/Vietnam/1203/04 virus with a Y161A mutations conferring Neu5Gc specificityDepositorInsertH5_Vietnam_1203_04_Y161A
UseTagsGCN4-TEV-sfGFP-TwinStrepExpressionMammalianMutationY161A mutant that confers exclusive NeuAc to NeuG…PromoterCMVAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-plk1-AKAP
Plasmid#132967PurposeTo study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damageDepositorUseTagsExpressionMammalianMutationC terminus of full-length Plk1 fused to the peric…PromoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-mtagBFP-2A STING-V155M S366A
Plasmid#102593PurposeLentivector to express STING V155M S366A and mTagBFPDepositorInsertSTING (STING1 Human)
UseLentiviralTagsExpressionMammalianMutationV155M S366APromoterSFFVAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
G protein alpha-s(alpha3beta5)
Plasmid#55799PurposeThis G protein alpha-s mutant exhibits increased affinity for and decreased ability to be activated by Gs-protein-coupled receptors as well as decreased ability to activate adenylyl cyclase.DepositorInsertG protein alpha-s with alpha-i2 substitutions in alpha3/beta5 loop (Gnas Rat)
UseTagsinternal EE epitope (residues 189-194 in alpha-s …ExpressionMammalianMutationN271K, K274D, R280K, T284D, I285T in alpha-s sequ…PromoterCMVAvailable sinceJuly 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV_EE-NG-nCas9
Plasmid#163542PurposeMammalian CG-to-GC base editingDepositorInsertEE-NG-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…PromoterAvailable sinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Con/Fon-bREACHes-EYFP
Plasmid#137145PurposeIntersectional viral expression of bREACHes-EYFP in cells expressing both Cre AND FlpDepositorInsertCon/Fon-bREACHes-EYFP
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationn/aPromoternEFAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX Emt-SH2
Plasmid#46424DepositorInsertEmt (ITK Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 223-346PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Lyn-SH2
Plasmid#46452DepositorInsertLyn (LYN Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 106-213PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDB-hPER2-Halo-CD4-bla
Plasmid#179451PurposeDonor vector to knock in Halotag C-terminal to human PER2 geneDepositorInsertHaloTag (Halo7 Synthetic)
UseCRISPRTagsHis/FlagExpressionMutationPromoterNoneAvailable sinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSH-EFIRES-P-GFP(1-10)opti
Plasmid#129416PurposeExpressing codon-optimized GFP(1-10) fragment in human cellsDepositorInsertcodon-optimized GFP(1-10)
UseCRISPR, TALEN, and Unspecified ; Human safe harbo…TagsExpressionMammalianMutationPromoterEF-1αAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV_UdgX-EE-UdgX-NG-nCas9-UdgX
Plasmid#163572PurposeMammalian CG-to-GC base editingDepositorInsertUdgX-EE-UdgX-NG-nCas9-UdgX
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…PromoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+ Flag-RAGB99L
Plasmid#128101PurposeExpresses constitutively GTP-bound RAGB in mammalian cells in a G418-selectable plasmidDepositorInsertRas related GTP binding B (RRAGB Human)
UseTagsFlagExpressionMammalianMutationQ99L (CAA to CTA)PromoterCMVAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBabe_puro_DEST_Flag_EIF5A2
Plasmid#45266DepositorInsertEIF5A2 (EIF5A2 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
Mouse DsRed-Monomer CASP9 C325A Mutant
Plasmid#198401PurposeExpresses Mouse DsRed-Monomer CASP9 C325A Mutant Fusion Protein in Mammalian CellsDepositorInsertMouse Caspase-9 C325A Mutant (Casp9 Mouse)
UseTagsDsRed-monomerExpressionMammalianMutationChanged Cysteine 325 to Alanine to eliminate prot…PromoterCMVAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX SHP-2(C)-SH2
Plasmid#46497DepositorInsertSHP-2(C) (PTPN11 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 101-222PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only