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Plasmid#135618PurposeDonor vector for genomic targeting of a P2A-CreERT2 cassette to the 3' end of the mouse Sox2 locusDepositorInsertP2A-CreERT2 flanked by Sox2 homology arms (Sox2 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsP2A-CreERT2ExpressionMutationPromoterAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgGabrg2
Plasmid#124857PurposeMutagenesis of Gabrg2DepositorInsertGabrg2 (Gabrg2 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pscAAV-Sox2-mStr
Plasmid#135617PurposeDonor vector for genomic targeting of a P2A-mStrawberry cassette to the 3' end of the mouse Sox2 locusDepositorInsertP2A-mStrawberry flanked by Sox2 homology arms (Sox2 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsP2A-mStrawberryExpressionMutationPromoterAvailable sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKrox24(2xD-E)dTomato
Plasmid#200110PurposeFluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activityDepositorInsertmodified EGR1 promoter
UseTagsExpressionMammalianMutationhighly modified sequencePromoter2 copies of D-E element, no other promoter elemen…Available sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
PDPN-bio-His
Plasmid#52313PurposeExpresses full-length Podoplanin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertPDPN (PDPN Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8666 pHR-RRM2p-BFP-T2A-N term (1-406) dLbCpf1-EE12RR345L-EFS-Zeo-WPRE
Plasmid#140228PurposeRRM2p-driven expression of BFP and N terminal dCpf1 (Cas12a) 406-split half with leucine zipper. Constitutive EFS-driven zeocin resistance.DepositorInsertdLbCpf1 (Cas12a) N terminal half (406) with zipper
UseLentiviralTagsBFPExpressionMammalianMutationPromoterRRM2 endogenous promoterAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR4 PARP2 E614Q
Plasmid#192545PurposepENTR plasmid Arabidopsis thaliana PARP2 CDS, E614Q mutation, no stop codonDepositorInsertpoly(ADP-ribose) polymerase 2 E614Q (AT3G18250 Mustard Weed)
UseGateway entry vectorTagsExpressionMutationchanged Glu 614 to GlnPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-hMeCP2
Plasmid#48091PurposeBacterial expression of Mxe intein/chitin binding domain tagged MeCP2-WTDepositorInsertMethyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
UseTagsExpressionBacterialMutationMxe intein/chitin binding domain taggedPromoterAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
ss-SNAP-TMD-NS3-Gal4
Plasmid#112279PurposeEncodes the membrane-targeted TMD-NS3-Gal4, which exhibits drug-inducible transcription "turn-off" activity. SNAP-tag is fused to the extracellular region of the encoded protein construct.DepositorInsertSNAP-TMD-NS3-Gal4
UseTagsAU1 (N term on NS3) and HA (C term on NS3)ExpressionMammalianMutationNS3 mutant T54APromoterCMVAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLX302 ID2-V5 puro
Plasmid#83096PurposeLentiviral vector for constitutive expression of human ID2 with C-terminal V5 tagDepositorInsertID2 (ID2 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-hMeCP2-GFP-R270X
Plasmid#48079PurposeMammalian expression of GFP tagged MeCP2-R270XDepositorInsertMethyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
UseTagsGFPExpressionMammalianMutationexpresses AT-Hook R270XPromoterCMVAvailable sinceApril 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
VIPR1
Plasmid#51865PurposeExpresses full-length Vasoactive intestinal polypeptide receptor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertVIPR1 (VIPR1 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast SHMT2res
Plasmid#106301PurposeExpresses SHMT2DepositorInsertserine hydroxymethyltransferase 2 (SHMT2 Human)
UseRetroviralTagsExpressionMammalianMutationSilent mutations destroy sgRNA targeting sitesPromoterAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
HOXA9-MSCV-CD24
Plasmid#20975DepositorUseRetroviralTagsFlagExpressionMammalianMutationFull length cDNA with ~1.1kb 3'UTR with 2 pu…PromoterAvailable sinceAug. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
MSCV-NSD3-short W284A
Plasmid#72551Purposeexpresses 3*FLAG tagged human NSD3-short with W284A mutationDepositorInsertNSD3-short (NSD3 Human)
UseRetroviralTags3*FLAGExpressionMammalianMutationW284APromoterLTRAvailable sinceJan. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMX-Gal4-hMeCP2-R270X
Plasmid#48083PurposeMammalian expression of Gal4 tagged MeCP2-R270XDepositorInsertMethyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
UseTagsGal4ExpressionMammalianMutationexpresses AT-Hook R270XPromoterAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDEST-hMeCP2-GFP-G273X
Plasmid#48080PurposeMammalian expression of GFP tagged MeCP2-G273XDepositorInsertMethyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
UseTagsGFPExpressionMammalianMutationexpresses AT-Hook G273XPromoterCMVAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8629 pHR-HREYBTATA-RR12EE345L-C term (406) dLbCpf1-2XNLS-miniVPR-mCherry-EFS-Puro-WPRE
Plasmid#140227PurposeHypoxia-inducible expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to miniVPR. Constitutive EFS-driven puromycin resistance.DepositorInsertzipper with dLbCpf1 (Cas12a) C terminal half (406) fused to miniVPR
UseLentiviralTagsExpressionMammalianMutationPromoterHRE (Hypoxia inducible)Available sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMX-Gal4-hMeCP2-R111G
Plasmid#48085PurposeMammalian expression of Gal4 tagged MeCP2-WT-R111GDepositorInsertMethyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
UseTagsGal4ExpressionMammalianMutationR111G mutant MeCP2PromoterAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEXfrt-SaCas9-U6-sgGabrg2
Plasmid#124871PurposeMutagenesis of Gabrg2DepositorInsertGabrg2 (Gabrg2 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only