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pET28a-DDX43
Plasmid#134574PurposeExpress human DDX43 protein (WT, full-length) in E. coliDepositorInsertDDX43 (DDX43 Human)
UseTagsHisExpressionBacterialMutationfull-length WTPromoterT7Available sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX SHP-1(C)-SH2
Plasmid#46494DepositorInsertSHP-1(C) (PTPN6 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 105-217PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Foff 2.0-oScarlet
Plasmid#137137PurposeIntersectional viral expression of oScarlet in cells expressing Cre AND NOT FlpDepositorHas ServiceAAV8InsertCon/Foff 2.0-oScarlet
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationE95DPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pACT1:Cas9-GFP, U6:sgUPRT
Plasmid#122853PurposeExpresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum UPRT geneDepositorInsertsCas9-GFP
U6-sgUPRT
UseCRISPR; Cas9-gfp plasmid for genome editing of cr…TagseGFPExpressionMutationPromoterCryptosporidium parvum U6 and Cryptosporidium par…Available sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV_UdgX-APOBEC1-UdgX-HF-nCas9
Plasmid#163559PurposeMammalian CG-to-GC base editingDepositorInsertUdgX-APOBEC1-UdgX-HF-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)HF (N497A, R661A, Q695A, Q926A)PromoterAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-MAP3K2
Plasmid#85652Purposeretroviral expression of human MAP3K2DepositorInsertMAP3K2 (MAP3K2 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterLTRAvailable sinceFeb. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLPB3B-mAID-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin-T2A-osTIR1
Plasmid#187963PurposemAID degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance, T2A site and osTIR1 under PGK promoter.DepositorInsertsAID-SpdCas9-tagRFPt-P2A-tagBFP; Blasticidin-T2A-osTIR1
osTIR1
UseCRISPR and Synthetic BiologyTagsGGS linker and T2AExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFRT/TO/HIS/FLAG/HA-CSNK1E
Plasmid#38078DepositorInsertCSNK1E casein kinase 1, epsilon (CSNK1E Human)
UseTagsHIS/FLAG/HAExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-mtagBFP-2A STING V155M 368STOP
Plasmid#102596PurposeLentivector to express STING V155M delta 368 and mTagBFPDepositorInsertSTING (STING1 Human)
UseLentiviralTagsExpressionMammalianMutationV155M delta 368PromoterSFFVAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX Stat1-SH2
Plasmid#46511DepositorInsertStat1 (STAT1 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 488-683PromoterTacAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
FUW-TetO-Klf5
Plasmid#128830Purposedox-dependent expression of mouse Klf5DepositorInsertKlf5 (Klf5 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX SHP-2(NC)-SH2
Plasmid#46499DepositorInsertSHP-2(NC) (PTPN11 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 1-222PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
FLAG-KLF12/pcDNA3.1-Neo
Plasmid#81069PurposeExpression of epitope-tagged human KLF12 in mammalian cells.DepositorInsertFLAG-KLF12 (KLF12 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
3019_pETcon-SARS-CoV-2-RBD_E484K
Plasmid#184404Purposeyeast surface display of the SARS-CoV-2 Eta variant RBDDepositorInsertSARS-CoV-2 Eta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
UseTagsHA and c-MycExpressionYeastMutationE484KPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso1_1-1605-shRNAres_W
Plasmid#147889PurposeMammalian Expression of HsNot1iso1_1-1605-shRNAresDepositorInsertHsNot1iso1_1-1605-shRNAres (CNOT1 Human)
UseTagsExpressionMammalianMutation1 silent mutation compared to the sequence of iso…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso1_1093-1317_W
Plasmid#147892PurposeMammalian Expression of HsNot1iso1_1093-1317 *Please note that this plasmid encodes amino acids 1093-1322 (not 1093-1317 as the name suggests).DepositorInsertHsNot1iso1_1093-1317 (CNOT1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso1_1-1840-shRNAres_W
Plasmid#147891PurposeMammalian Expression of HsNot1iso1_1-1840-shRNAresDepositorInsertHsNot1iso1_1-1840-shRNAres (CNOT1 Human)
UseTagsExpressionMammalianMutation1 silent mutation compared to the sequence of iso…PromoterAvailable sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Con/Foff 2.0-ChR2-mCherry
Plasmid#137143PurposeIntersectional viral expression of ChR2-mCherry in cells expressing Cre AND NOT FlpDepositorHas ServiceAAV8InsertCon/Foff 2.0-ChR2-mCherry
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationH134RPromoternEFAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX Btk-SH2
Plasmid#46412DepositorInsertBtk (BTK Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 271-385PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only
