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Plasmid#62348PurposeAn empty optimized gRNA expression vectorDepositorInsertnone
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
Construct 31 - 1xTetO_H1p_20nt2_stgRNA_3xLacO_U6p_20nt3_stgRNA
Plasmid#81254PurposeFor expressing Dox and IPTG inducible stgRNAsDepositorInsertstgRNA
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRec ΔLacI
Plasmid#104575PurposeExpresses Cas1, Cas2 under PLTetO-1 promoter, and TetR constitutively.DepositorInsertPTet-cas12, tetR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide1
Plasmid#118158PurposeCRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the EGFP CDSDepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterEF1a core and U6Available sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pATF4-donor
Plasmid#113805PurposeCRISPR donor plasmid to tag human transcription factor ATF4 with GFPDepositorInsertATF4 homology arms flanking EGFP-IRES-Neo cassette (ATF4 Human)
UseCRISPRTagsExpressionMutationrs140428747PromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3s-Cas9HC
Plasmid#43945DepositorInsertCas9
UseCRISPRTagsHA epitope and NLSExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFH14
Plasmid#125799PurposeLevel0 SaCas9, Arabidopsis codon optimizedDepositorInsertSaCas9, Arabidopsis codon optimized
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH13
Plasmid#125798PurposeLevel0 SpCas9, wheat codon optimizedDepositorInsertSpCas9, wheat codon optimized
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH17
Plasmid#125803PurposeLevel0 LbCas12a, rice codon optimizedDepositorInsertLbCas12a, rice codon optimized
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRec
Plasmid#104572PurposeExpresses Cas1, Cas2 under PLTetO-1 promoter, and TetR and LacI constitutively.DepositorInsertPTet-cas12, tetR, lacI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFOXF2-donor
Plasmid#113816PurposeCRISPR donor plasmid to tag human transcription factor FOXF2 with GFPDepositorInsertFOXF2 homology arms flanking EGFP-IRES-Neo cassette (FOXF2 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRB1080
Plasmid#71309PurposeExpresses VQR Cas9 variant with eef-1A.1 (eft-3) promoterDepositorInsertVQR Cas9 variant
UseCRISPRTagsExpressionWormMutationPromotereef-1A.1 (eft-3)Available sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
ttHsCas12a
Plasmid#182385PurposeGives high editing efficiency in barley.DepositorInsertttHsCas12a
UseCRISPR; Coding sequence onlyTagsExpressionMutationD156RPromoterNoneAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
UAST-LbCas12a
Plasmid#140621PurposeGal4 dependent expression of LbCas12a in DrosophilaDepositorInsertLbCas12a
UseCRISPRTagsExpressionInsectMutationPromoterUASTAvailable sinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV:AsCpf1-2A-GFP-U6-sgRNA-cloning vector
Plasmid#194715PurposeBicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that can be cloned with BbsI sitesDepositorArticleInsertAsCpf1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.3TOPO-T7-hCas9
Plasmid#161876PurposeIn vitro transcription of SpCas9DepositorInserthuman codon optimized SpCas9
UseTagsExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceSept. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX330-Puro-ccdB
Plasmid#82580PurposeA modified version of plasmid pX330 (#42230): Added are a ccdB stuffer and a Puromycin selection cassetteDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCBhAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEX-A-U6-TelgRNA_PuroR
Plasmid#84782PurposeExpresses telomere-specific sgRNA.DepositorInserttelomere-specific sgRNA
UseCRISPRTagsExpressionBacterial and MammalianMutationPromoterU6Available sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
1x_a1gRNAOp_pCYC1m_yeBFP2
Plasmid#64390Purposeencodes one a1 operator site upstream of minimal pCYC1 promoter driving yeast enhanced BFPDepositorInsertyeast enhanced BFP2
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pESR1-donor
Plasmid#112337PurposeCRISPR donor plasmid to tag human transcription factor ESR1 with GFPDepositorInsertESR1 homology arms flanking EGFP-IRES-Neo cassette (ESR1 Human)
UseCRISPRTagsExpressionMutationUnknownPromoterAvailable sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only