-
Plasmid#73430PurposeCRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1B6.DepositorInsertRepressor 1B6 (orthogonal T7-lac repressor)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterConstitutive wild-type S. pyogenes promoterAvailable sinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-M-3H5
Plasmid#73429PurposeCRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3H5.DepositorInsertRepressor 3H5 (orthogonal T7-lac repressor)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterConstitutive wild-type S. pyogenes promoterAvailable sinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-M-1D4
Plasmid#73433PurposeCRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1D4.DepositorInsertRepressor 1D4 (orthogonal T7-lac repressor)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterConstitutive wild-type S. pyogenes promoterAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-M-1E4
Plasmid#73436PurposeCRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1E4.DepositorInsertRepressor 1E4 (orthogonal T7-lac repressor)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterConstitutive wild-type S. pyogenes promoterAvailable sinceMarch 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-M-5F5
Plasmid#73432PurposeCRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 5F5.DepositorInsertRepressor 5F5 (orthogonal T7-lac repressor)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterConstitutive wild-type S. pyogenes promoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
CCR5-SZ218a QTY
Plasmid#162444PurposeExpression in E coli and perform ligand binding in solutionDepositorInsertC-C chemokine receptor type 5 (CCR5 Human)
UseTagshis-tagExpressionBacterialMutationAll L, I, V, F in the transmembrane region mutate…PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
CCR5-SZ190b QTY
Plasmid#162445PurposeExpression in E coli and perform ligand binding in solutionDepositorInsertC-C chemokine receptor type 5 (CCR5 Human)
UseTagshis-tagExpressionBacterialMutationAll L, I, V, F in the transmembrane region mutate…PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pDONR223_CD70_WT
Plasmid#82003PurposeGateway Donor vector containing CD70, part of the Target Accelerator Plasmid Collection.DepositorInsertCD70 (CD70 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_NOTCH1_ICN
Plasmid#82087PurposeGateway Donor vector containing NOTCH1 , part of the Target Accelerator Plasmid Collection.DepositorInsertNOTCH1 (NOTCH1 Human)
UseGateway entry vectorTagsExpressionMutationICN (AA1761-2556 present)PromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual wt p110α/p85α
Plasmid#199302Purposeco-expression of His-tagged p110α wt and Avi-tagged p85α wt proteinsDepositorUseTagsAvi and HisExpressionInsectMutationPromoterAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_ERN1_WT
Plasmid#82115PurposeGateway Donor vector containing ERN1 , part of the Target Accelerator Plasmid Collection.DepositorInsertERN1 (ERN1 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag PIK3R5
Plasmid#20576DepositorInsertPIK3R5 (PIK3R5 Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag TTK
Plasmid#20659DepositorInsertTTK (TTK Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-sumo-h-cGAS
Plasmid#127161Purposeexpression of human cGAS proteinDepositorInsertMB21D1 (CGAS Human)
UseTagsHis-SUMO tagExpressionBacterialMutationNonePromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_BRAF_p.V600E
Plasmid#82186PurposeGateway Donor vector containing BRAF , part of the Target Accelerator Plasmid Collection.DepositorInsertBRAF (BRAF Human)
UseGateway entry vectorTagsExpressionMutationV600EPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_CD40_WT
Plasmid#82198PurposeGateway Donor vector containing CD40 , part of the Target Accelerator Plasmid Collection.DepositorInsertCD40 (CD40 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_BMPR1B_p.Q203D
Plasmid#82173PurposeGateway Donor vector containing BMPR1B , part of the Target Accelerator Plasmid Collection.DepositorInsertBMPR1B (BMPR1B Human)
UseGateway entry vectorTagsExpressionMutationQ203DPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBK2044-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2
Plasmid#223164Purpose2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and empty gRNA scaffoldDepositorInsertKRAB-MECP2 (MECP2 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 204-310 (MECP2)PromoterEF1aAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_ETS1_WT
Plasmid#82118PurposeGateway Donor vector containing ETS1 , part of the Target Accelerator Plasmid Collection.DepositorInsertETS1 (ETS1 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_CYLD_WT
Plasmid#82326PurposeGateway Donor vector containing CYLD , part of the Target Accelerator Plasmid Collection.DepositorInsertCYLD (CYLD Human)
UseGateway entry vectorTagsExpressionMutationPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only