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pENTR-GFP-WT1 -KTS N terminal HA

Plasmid

#217641

Purpose

Entry vector with WT1 -KTS mutant with N terminal HA for gateway cloning

Depositor

Constanze Bonifer

Article

Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

Insert

WT1 -KTS isoform (WT1 Human)

Use

Gateway cloning

Tags

HA

Expression

Mutation

p.408_410delKTS

Promoter

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-GFP-WT1 -KTS C terminal HA

Plasmid

#217642

Purpose

Entry vector with WT1 -KTS mutant with C terminal HA for gateway cloning

Depositor

Constanze Bonifer

Article

Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

Insert

WT1 -KTS isoform (WT1 Human)

Use

Gateway cloning

Tags

HA

Expression

Mutation

p.408_410delKTS

Promoter

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pACEBac1-PSMB5-PSMB6-PSMB7

Plasmid

#217898

Purpose

Insect cell expression of human 20S CP proteasome subunit beta type-5 (PSMB5), beta type-6 (PSMB6), and beta type-7 (PSMB7)

Depositor

Brenda Schulman

Article

Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

Inserts

PSMB5 (PSMB5 Human)
PSMB6 (PSMB6 Human)
PSMB7 (PSMB7 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pJH4562

Plasmid

#200780

Purpose

Pflp-18 LoxP EBFP LoxP GtACR2 mCherry unc-54 3' UTR C.elegans AVA and other neurons expression of GtACR2 RFP

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

GtAR2

Use

Tags

mCherry

Expression

Worm

Mutation

Promoter

Pflp-18

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pJH4609

Plasmid

#200781

Purpose

Ppdf-1 LoxP EBFP LoxP GtACR2 mCherry unc-54 3' UTR C.elegans AVB and other neurons expression of GtACR2 RFP

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

GtACR2

Use

Tags

mCherry

Expression

Worm

Mutation

Promoter

Ppdf-1

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pJH4677

Plasmid

#200782

Purpose

Pnpr-4 ins-22::GFP unc-54 3' UTR C.elegans AVA and other neurons expression of ins-22 GFP

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

ins-22 (ins-22 Nematode)

Use

Tags

GFP

Expression

Worm

Mutation

Promoter

Pnpr-4

Available since

May 7, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pJH4783

Plasmid

#200785

Purpose

Pnpr-4 snb-1::pHluorin unc-54 3' UTR C.elegans AVA and other neurons expression of snb pHluorin

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

snb-1 (snb-1 Nematode)

Use

Tags

pHiuorin

Expression

Worm

Mutation

Promoter

Pnpr-4

Available since

May 7, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pJH4169

Plasmid

#200108

Purpose

Prig-3 FRT let858 (stop) FRT snb-1::GFP unc-54 3' UTR C.elegans AVA and other neurons expression of snb GFP

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

snb-1 (snb-1 Nematode)

Use

Tags

GFP

Expression

Worm

Mutation

Promoter

Prig-3

Available since

May 7, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pJH3830

Plasmid

#200038

Purpose

Prig-3 zif-1 SL2 mCherry unc-54 3' UTR C.elegans AVA and other neurons expression of zif RFP

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

ZIF (zif-1 Nematode)

Use

Tags

mCherry

Expression

Worm

Mutation

Promoter

Prig-3

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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PB-LacI-eGFP

Plasmid

#213021

Purpose

piggyBac donor vector containing LacI-eGFP for random integration by PBase

Depositor

Luca Giorgetti

Article

Mach et al Nat Genet. 2022 Dec;54(12):1907-1918. do

Insert

LacI**-eGFP

Use

Mouse Targeting; Piggybase integration (transposo…

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pGEL635

Plasmid

#189829

Purpose

The T-DNA backbone of Cas12j2 for genome editing in rice

Depositor

Yong Zhang

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR; T-dna clone

Tags

ccdb

Expression

Mutation

Promoter

ZmUbi1

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGEL637

Plasmid

#189832

Purpose

The T-DNA backbone of vCas12j2 for genome editing in rice

Depositor

Yong Zhang

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR; T-dna clone

Tags

Expression

Mutation

Promoter

ZmUbi1

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGEL639

Plasmid

#189833

Purpose

The T-DNA backbone of Cas12j2 for DNA methylation-based gene silencing in rice

Depositor

Yong Zhang

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR; T-dna clone

Tags

Expression

Mutation

Promoter

ZmUbi1

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-FRT-TO-NUbo-E3(63)-mCherry-FLAG

Plasmid

#212809

Purpose

Constitutive or doxycycline-inducible expression of NUbo-E3(63)-mCherry-FLAG in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(63)

Use

Tags

NUbo and mCherry-FLAG

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac1-His6-E3(1)-CUbo-TwinStrep

Plasmid

#212712

Purpose

Expression of His6-E3(1)-CUbo-TwinStrep in insect cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(1)

Use

Tags

6xHis and CUbo-TwinStrep

Expression

Bacterial

Mutation

Promoter

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-NUbo-E3(48)-mCherry-FLAG

Plasmid

#212803

Purpose

Constitutive or doxycycline-inducible expression of NUbo-E3(48)-mCherry-FLAG in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(48)

Use

Tags

NUbo and mCherry-FLAG

Expression

Mammalian

Mutation

Promoter

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
YIp128-TET-NUbo-E3(63)-VSV

Plasmid

#212752

Purpose

Doxycycline-inducible expression of NUbo-E3(63)-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(63)

Use

Integrative vector

Tags

NUbo and VSV

Expression

Yeast

Mutation

Promoter

Tet

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
YIp211-TET-NUbo-E3(63)-VSV

Plasmid

#212753

Purpose

Doxycycline-inducible expression of NUbo-E3(63)-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(63)

Use

Integrative vector

Tags

NUbo and VSV

Expression

Yeast

Mutation

Promoter

Tet

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
YIp211-TET-NUbo-E3(48)-VSV

Plasmid

#212744

Purpose

Doxycycline-inducible expression of NUbo-E3(48)-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(48)

Use

Integrative vector

Tags

NUbo and VSV

Expression

Yeast

Mutation

Promoter

Tet

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
YIp128-TET-NUbo-E3(48)-VSV

Plasmid

#212743

Purpose

Doxycycline-inducible expression of NUbo-E3(48)-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(48)

Use

Integrative vector

Tags

NUbo and VSV

Expression

Yeast

Mutation

Promoter

Tet

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge