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Plasmid#112926PurposeCRISPR-a reporter assay lentiviral vector (empty gRNA control)DepositorInserthU6-gRNASAM(empty), TREGFP, PGKpuroGFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTRE2-Bla(HA–RILP-FLAG)
Plasmid#102424PurposeDual tagged RILP for expression in mammalian cells.DepositorInsertRab interacting lysosomal protein (RILP Human)
UseTagsFLAG and HAExpressionMammalianMutationPromoterTet-ResponsiveAvailable sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2_N_deltaCTD
Plasmid#157871Purposeexpress MBP tagged SARS-CoV-2 Nucleocapsid protein without the C-terminal domainDepositorInsertN (N SARS-CoV-2)
UseTagsMBPExpressionBacterialMutationdeltaCTDPromoterAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2 hSmad1-EVE-MM
Plasmid#22994DepositorInsertSmad1 (SMAD1 Human)
UseTagsFLAGExpressionMammalianMutationS214A, S206A, S195A, S187A, S463E, S465EPromoterAvailable sinceJune 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
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pMTP140 (pBAD322G_TniQ-Cascade(Tn6900))
Plasmid#164262PurposeVector expressing the TniQ, Cas8/5, Cas7, and Cas6 proteins from the Tn6900 element with an arabinose promoter.DepositorInsertCodon-optimized tniQ-cas8/5-cas7-cas6 operon from Tn6900 from A. salmonicida S44 pS44-1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDOC-K-glmS-mPlum
Plasmid#158062PurposeDonor plasmid for insertion of mPlum and the constitutive acpP promoter into the S. Typhimurium chromosome. mPlum is in the forward orientation relative to the kanamycin resistance selectable markerDepositorInsertmonomeric plum fluoresecent protein
UseSynthetic BiologyTagsExpressionBacterialMutationPromoteracpPAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-NES-MBP-cATOM(Ras)
Plasmid#209711PurposeMammalian expression of c-ATOM biosensor for HRas with nuclear export signalDepositorInsertNES-MBP-cATOM(Ras)
UseTagsNES-MBPExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFosill-3
Plasmid#89697Purposeto make Fosmids that can be converted into Illumina sequencable librariesDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMP4518
Plasmid#107778PurposeExpression of EYFPDepositorInsertpLacZ-EYFP in pBBR1-mcs5
UseTagspLacZ-EYFPExpressionBacterialMutationPromoterpLacZAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only