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Plasmid#138188Purpose3rd generation lentiviral gRNA plasmid targeting human KAT5DepositorInsertKAT5 (KAT5 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDB-hCRY1-mClover3-CD4-bla
Plasmid#179441PurposeDonor vector to knock in mClover3 C-terminal to human CRY1 geneDepositorInsertmClover3
UseCRISPRTagsHis/FlagExpressionMutationPromoterNoneAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGV3340
Plasmid#170278PurposeExpression of mutant HIV-2 RT p51 reverse transcriptaseDepositorInsertHIV-2 RT p51 (mutant)
UseTagsHexahistidineExpressionBacterialMutationPromoterT7-lacAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATX3-13Q
Plasmid#184248PurposeRecombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track fused with His-Tag and TEV cleavage sequence (N-terminal)DepositorInsertataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track (ATXN3 Human, Synthetic)
UseTags6x His-Tag and TEV cleavage siteExpressionBacterialMutationC-terminal codon optimization for protein expres…PromoterT7 promoterAvailable sinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
FLAG-mCep290
Plasmid#27381DepositorInsertCep290 (Cep290 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceFeb. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLentiCrisprV2 sgRNA hPER2c-term #1
Plasmid#179454PurposeLentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-inDepositorInsertsgRNA targeting human PER2
UseCRISPR and LentiviralTagsExpressionMutationPromoterhU6Available sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLentiCrisprV2 sgRNA hPER2c-term #2
Plasmid#179455PurposeLentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-inDepositorInsertsgRNA targeting human PER2
UseCRISPR and LentiviralTagsExpressionMutationPromoterhU6Available sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLentiCrisprV2 sgRNA hPER2c-term #3
Plasmid#179456PurposeLentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-inDepositorInsertsgRNA targeting human PER2
UseCRISPR and LentiviralTagsExpressionMutationPromoterhU6Available sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLentiCrisprV2 sgRNA hCRY1 c-term
Plasmid#179453PurposeLentiviral Crispr/Cas9 plasmid targeting hCRY1 at C-terminus to generate C-terminal knock-inDepositorInsertsgRNA targeting human CRY1
UseCRISPR and LentiviralTagsExpressionMutationPromoterhU6Available sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF-DEST51-GOLPH3
Plasmid#21690DepositorInsertGolgi Phosphoprotein 3 (GOLPH3 Human)
UseInvitrogen gateway destination vectorTags6xHIS and V5ExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
J-sRNA-331Bb
Plasmid#78285PurposeAn inducible small RNA with E.coli Hfq targeting K. rhaeticus UGPase mRNADepositorInsertj-sRNA-331
UseTagsExpressionMutationPromoterAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Fon-GCaMP6F
Plasmid#137122PurposeIntersectional viral expression of GCaMP6F in cells expressing both Cre AND FlpDepositorHas ServiceAAV5 and AAV8InsertCon/Fon-GCaMP6F
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationRemoved RSET tagPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJHG003
Plasmid#126454PurposeExpresses Cas9-XTEN-dTdT in mammalian cells.DepositorInsertCas9-XTEN-dTdT (DNTT Human, Streptococcus pyogenes)
UseTagsExpressionMammalianMutationAdded D343E and D345E to inactivate TdT.PromoterCMVAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Foff 2.0-GCaMP6F
Plasmid#137123PurposeIntersectional viral expression of GCaMP6F in cells expressing Cre AND NOT FlpDepositorHas ServiceAAV8InsertCon/Foff 2.0-GCaMP6F
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationRemoved RSET tagPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Coff/Fon-mCherry
Plasmid#137134PurposeIntersectional viral expression of mCherry in cells expressing Flp AND NOT CreDepositorHas ServiceAAV8InsertCoff/Fon-mCherry
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationn/aPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV_Anc689-NG-nCas9
Plasmid#163527PurposeMammalian CG-to-GC base editingDepositorInsertAnc689-NG-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…PromoterAvailable sinceDec. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Con/Foff 2.0-Arch3.3-EYFP
Plasmid#137149PurposeIntersectional viral expression of Arch3.3-EYFP in cells expressing Cre AND NOT FlpDepositorHas ServiceAAV8InsertCon/Foff 2.0-Arch3.3-EYFP
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationn/aPromoternEFAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNL-CEF-IgM-IRES-GFP
Plasmid#187011PurposeExpression of human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgMDepositorInsertHuman anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgM
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNL-CEF-CD79A-YFP
Plasmid#187004PurposeExpression of human CD79A as YFP fusion proteinDepositorInsertCD79A (CD79A Human)
UseLentiviralTagsYFPExpressionMutationPromoterAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
FUW-TetO-Gata6
Plasmid#128827Purposedox-dependent expression of mouse Gata6DepositorInsertGata6 (Gata6 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only