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Plasmid#73590PurposeExpress C-terminal part (Q391- end) of human TRAFD1(FLN29) in mammalian cellsDepositorInsertTRAFD1 (TRAFD1 Human)
UseTags3xFlagExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
hFLN29-Mid/pCMV-14-FLAG (L175-D390)
Plasmid#73589PurposeExpress middle part (L175-D390) of human TRAFD1 in mammalian cellsDepositorInsertTRAFD1 (TRAFD1 Human)
UseTags3xFlagExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
hFLN29-N/pCMV-14-FLAG (1-I210)
Plasmid#73588PurposeExpress N-terminal part (1-I210) of hTRAFD1 (FLN29) in mammalian cellsDepositorInsertTRAFD1 (TRAFD1 Human)
UseTags3xFlagExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pED54pRS306 pRPS2-Ste7DS NLS(4E)-mCherry
Plasmid#68276PurposeSKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS(4E) mCherry Non functional sensor with phospho-mimicking NLSDepositorInsertSte7DS NLS(4E) mCherry
UseTagsmCherryExpressionYeastMutationPromoterRPS2Available sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
Gg 3kb Green opsin GFP
Plasmid#72919Purposegreen opsin promoter from chicken (Gallus gallus) gDNA chr26:4,504,913–4,501,931 in galGal4 driving GFPDepositorInsertchicken green opsin promoter
UseTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Gg 3kb Green opsin DsRed
Plasmid#72918Purposegreen opsin promoter from chicken (Gallus gallus) gDNA chr26:4,504,913–4,501,931 in galGal4 driving DsRedDepositorInsertchicken green opsin promoter
UseTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR - C16orf80 sgRNA 1
Plasmid#70651PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C16orf80 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.DepositorInsertsgRNA against C16orf80
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR - C16orf80 sgRNA 2
Plasmid#70650PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C16orf80 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.DepositorInsertsgRNA against C16orf80
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR - C3orf17 sgRNA 2
Plasmid#70653PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C3orf17 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.DepositorInsertsgRNA against C3orf17
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-RTRG
Plasmid#69845PurposeThe RTRG repeat act as a nuclear/cytoplasmic partition signal in the expressed fusion protein providing increased nuclear to cytoplasmic partition ratio when increasing the number of repeatsDepositorInsert1 RTRG repeat
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-(RTRG)2
Plasmid#69846PurposeThe RTRG repeat act as a nuclear/cytoplasmic partition signal in the expressed fusion protein providing increased nuclear to cytoplasmic partition ratio when increasing the number of repeatsDepositorInsert2 RTRG repeats
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-(RTRG)3
Plasmid#69847PurposeThe RTRG repeat act as a nuclear/cytoplasmic partition signal in the expressed fusion protein providing increased nuclear to cytoplasmic partition ratio when increasing the number of repeatsDepositorInsert3 RTRG repeats
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-(RTRG)4
Plasmid#69848PurposeThe RTRG repeat act as a nuclear/cytoplasmic partition signal in the expressed fusion protein providing increased nuclear to cytoplasmic partition ratio when increasing the number of repeatsDepositorInsert4 RTRG repeats
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pED55 pRS306 pRPS2-SteND-NLS-mCherry
Plasmid#68277PurposeSKARS: Fus3 and Kss1 MAPK activity sensor: Ste7ND 2xNLS mCherry Non functional sensor with non-docking mutationDepositorInsertSte7ND NLS mCherry
UseTagsmCherryExpressionYeastMutationPromoterpRPS2Available sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pED53 pRS306 pRPS2-Ste7DS NLS(4A)-mCherry
Plasmid#68275PurposeSKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS(4A) mCherry Non functional sensor with non-phosphorylatable NLSDepositorInsertSte7DS NLS(4A) mCherry
UseTagsmCherryExpressionYeastMutationPromoterpRPS2Available sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUNC2001
Plasmid#67280PurposeExpresses MBP-Vibrio cholera photolyase in E. coli, vector pMalcwDepositorInsertVibrio cholera photolyase
UseTagsmaltose binding proteinExpressionBacterialMutationnone knownPromoterAvailable sinceJuly 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBGSA FPR1 R123A
Plasmid#66721PurposeConstitutive expression of FPR1 R123A in mammalian cells.DepositorInsertFPR1 (haplotype 3) (FPR1 Human)
UseTagsExpressionMammalianMutationR123APromoterSR-alphaAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBGSA FPR1 D71A
Plasmid#66720PurposeConstitutive expression of FPR1 D71A in mammalian cells.DepositorInsertFPR1 (haplotype 3) (FPR1 Human)
UseTagsExpressionMammalianMutationD71APromoterSR-alphaAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR H1 R4-R3
Plasmid#62105PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a H1 promoter for shRNA expression. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseRNAi; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIhh4.5k-Luc
Plasmid#53603PurposeReporter construct containing a 4.5 kb promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assaysDepositorInsertIhh promoter
UseLuciferase; ReporterTagsExpressionMutationPromoterAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only