pBGSA FPR1 D71A
(Plasmid
#66720)
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PurposeConstitutive expression of FPR1 D71A in mammalian cells.
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 66720 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBSSK+
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 7200
- Total vector size (bp) 8520
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Modifications to backboneExtensively modified
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFPR1 (haplotype 3)
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Alt nameFMLP-R26
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SpeciesH. sapiens (human)
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Insert Size (bp)1320
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MutationD71A
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GenBank IDM60627
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Entrez GeneFPR1 (a.k.a. FMLP, FPR)
- Promoter SR-alpha
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer 5'-CTTCTAGGCCTGTACGG-3'
- 3′ sequencing primer 5'-TCTAGAACTAGTGGATCC-3' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This mutant binds ligand but has a signaling defect. Also associated with PMID: 9378994.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBGSA FPR1 D71A was a gift from Heini Miettinen (Addgene plasmid # 66720 ; http://n2t.net/addgene:66720 ; RRID:Addgene_66720) -
For your References section:
Identification of putative sites of interaction between the human formyl peptide receptor and G protein. Miettinen HM, Gripentrog JM, Mason MM, Jesaitis AJ. J Biol Chem. 1999 Sep 24;274(39):27934-42. PubMed 10488141