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Plasmid#73572PurposeMammalian retroviral expression of Atoh1DepositorInsertAtoh1 (Atoh1 Mouse)
UseRetroviralTagsIRES-GFPExpressionMammalianMutationPromoterMSCVAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IPU-GFP-NEK9W967A
Plasmid#168271PurposeExpresses LIR (LC3-interacting region) mutant NEK9DepositorInsertNEK9 (NEK9 Human)
UseTagsFLAGExpressionMammalianMutationchanged tryptophan 979 to alaninePromoterAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN(E12,14K)-mPAGFP
Plasmid#168495PurposeMammalian expression of the E12,14K mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertE12,14K mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/Puro/FA/GFP-N--Grb2-4
Plasmid#112839Purposetransient/stable overexpressionDepositorInsertGrb2 (Grb2 Mouse)
UseTagsGFPExpressionMammalianMutationSH3 (N-term) domain of Grb2PromoterAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
gfp-Arkadia/ RNF111 deltaRING
Plasmid#112229PurposeExpresses GFP-tagged enzymatically inactive (delta RING) Arkadia in mammalian cellsDepositorInsert(Arkadia)Rnf111 (Rnf111 Mouse)
UseTagsGFPExpressionMammalianMutationdelta RING domain deletion (Deletion of the last …PromoterAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
AB.pCCL.sin.cPPT.U6.miR-182-Decoy.hPGK.GFP.WPRE
Plasmid#46596DepositorInsertmiR-182 Decoy
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
cTRE-GFP-miR30_shPten.1523
Plasmid#135667PurposeIntroduce Dox-inducible (TRE promoter) GFP cDNA plus miR30-embedded shPten into (ES) cells by recombination-mediated cassette exchangeDepositorInsertshPten
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
lenti-Cas9-VQR-GFP_activity_reporter
Plasmid#87156PurposeThis lentiviral vector can be used to assay activity of SpCas9-VQR (NGA PAM restricted).DepositorInsertGFP and sgRNA targeting GFP (NGA restricted)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBS537 tet-hCMV-GFPcre
Plasmid#11960DepositorInsertGFP-cre (cre bacteriophage P1)
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
Nano-lantern-Actin/pEGFP-C1
Plasmid#51972Purposemammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, fused to actinDepositorInsertNano-lantern
UseTagsActinExpressionMammalianMutationS257G in Rluc8PromoterAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
PWPXLD-GFP-Numb-S276D
Plasmid#133752PurposeNumb with mutation at serine 276 converted to aspartic acid cand tagged with GFP cloned into PWPXLDDepositorInsertNUMB endocytic adaptor protein (Numb Mouse)
UseLentiviralTagsGFPExpressionMammalianMutationserine 276 converted to aspartic acidPromoterEF1-alphaAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
mPA-GFP-mPlum-FRET-10
Plasmid#58154PurposeLocalization: Cytosolic FRET, Excitation: 504 / 590, Emission: 517 / 649DepositorTypeEmpty backboneUseTagsmPA-GFP and mPlumExpressionMammalianMutationPromoterAvailable sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmDCP2-V5His6
Plasmid#22410DepositorInsertdecapping protein 2, variant A (DCP2 Fly)
UseDrosophila expressionTagsEGFP and V5-His6ExpressionMutationPromoterAvailable sinceNov. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
Venus-7aa-Cb5-pEGFP-C1
Plasmid#166764PurposeTo target Venus to the Endoplasmic Reticulum. Cb5 tail anchor inserts into the membrane and Venus faces the cytosol. This serves as a collisional control in FLIM-FRET experiments.DepositorInsertCb5 tail anchor (Cyb5a Rat)
UseTagsVenusExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIRES LifeAct-GFP neo3
Plasmid#64356PurposeBicistronic expression of LifeAct-GFP fusion and neo3 resistance geneDepositorInsertLifeAct-GFP
UseTagsIRES-NeoExpressionMammalianMutationPromoterCMVAvailable sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV-U6-UL29-egfp-U3-UL8
Plasmid#166694PurposeTo express gRNA expression cassette simultaneously targeting two genes: UL8 and UL29 (EGFP version).DepositorUseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
AB.pCCL.sin.cPPT.U6.miR-335-5p-Decoy.hPGK.GFP.WPRE
Plasmid#46612DepositorInsertmiR-335-5p Decoy
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
1183_pAAV-U6-Ex14-gRNAd-CB-EmGFP
Plasmid#89061PurposeAAV-gRNA targeting the murine Ldlr geneDepositorInsertEmGFP
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterChicken beta actin with partial CMV enhancerAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;dll4CE2-P1Egfp
Plasmid#90147Purposecontains dll4 enhancer 2 sequence, and dll4 specific gene promoter driving expression of eGFPDepositorInsertdll4E2P1Egfp (dll4 Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSil-ETN-ires-GFP
Plasmid#58831PurposeExpress ETN version of rabies virus glycoprotein and GFP from CAG promoterDepositorInsertETN version of Rabies glycoprotein external domain fused to normal Rabies glycoprotein cellular domain
UseTagsires-EGFPExpressionMammalianMutationPromoterCAGAvailable sinceMarch 20, 2015AvailabilityAcademic Institutions and Nonprofits only