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Plasmid#121042PurposeMoClo golden gate assembly AE destination vector DVCK-AE-GFP (ColE1/kan resistance destination vector with constitutive GFP expression for screening.DepositorInsertKan destination vector with ColE1 origin and GFP selection
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pM1s3AsR_TS-C7
Plasmid#137945PurposeE. coli Nissle pMUT1-derived plasmid with ampicillin resistance and constitutive RFP expression and strong temperature sensitive GFP expressionDepositorInsertmRFP1
UseSynthetic Biology; For use with e. coli nissle in…TagsExpressionBacterialMutationPromoterJ23101Available sinceApril 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pM1s3AsG
Plasmid#137921PurposeE. coli Nissle pMUT1-derived plasmid with ampicillin resistance and constitutive GFP expressionDepositorInsertSuperfolder GFP
UseSynthetic Biology; For use with e. coli nissle in…TagsExpressionBacterialMutationPromoterJ23101Available sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pM2s2AsG
Plasmid#137924PurposeE. coli Nissle pMUT2-derived plasmid with ampicillin resistance and constitutive GFP expressionDepositorInsertSuperfolder GFP
UseSynthetic Biology; For use with e. coli nissle in…TagsExpressionBacterialMutationPromoterJ23101Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pM1s3ATScsg-Etag
Plasmid#137946PurposeE. coli Nissle pMUT1-derived plasmid with ampicillin resistance and temperature sensitive curli expressionDepositorInsertsSynthetic curli operon
Synthetic curli operon
UseSynthetic Biology; For use with e. coli nissle in…TagsExpressionBacterialMutationPromoterpTlpA36Available sinceApril 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pM2s2AsR_TS-E10
Plasmid#137944PurposeE. coli Nissle pMUT2-derived plasmid with ampicillin resistance and constitutive RFP expression and strong temperature sensitive GFP expressionDepositorInsertsmRFP1
mWasabi
UseSynthetic Biology; For use with e. coli nissle in…TagsExpressionBacterialMutationPromoterJ23101 and pTlpA-C7Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCryptDel4.8
Plasmid#141293PurposePlasmid for curing pMUT2 in one step based on pFREE. Contains RelB antitoxin, as well as gRNA targetting pMUT2 plasmid as well as pCryptDel4.8 itself.DepositorInsertsRelB
gRNA targetting pMUT2
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterNative promoter from pMUT2 relB/relE operon and P…Available sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
FL-DYSF-pUC57
Plasmid#60177PurposePlasmid contains full-length human dysferlin in a multiple cloning site of pUC57-Kan and can be easily cloned into other vectors.DepositorInsertDysferlin (DYSF Human)
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSFS2A-CaHygB
Plasmid#189564PurposeCaHygB hygromycin resistance gene in the pSFS2A flipper backbone for genetic manipulation of Candida albicansDepositorInsertHygB
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDonor_Tn[GFP]
Plasmid#213903PurposeMariner transposon expressing mNeonGreen with kan selection casetteDepositorInsertmariner transposon with kan resistance and mNeonGreen
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKM402
Plasmid#107770PurposeExpression of phage Che9c RecT for oligo-mediated recombineering in mycobacteriaDepositorInsertsChe9C RecT
TetR (tet repressor)
cat
kan
UseTagsExpressionBacterialMutationPromoterCat promoter, Ptet, kan promoter, and pTB21Available sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDonor_Tn[sfGFP]
Plasmid#213902PurposeMariner transposon expressing sfGFP with kan selection casetteDepositorInsertmariner transposon with kan resistance and superfolder GFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
T1P/pUBQ10(mTurq->mScarlet)
Plasmid#195945PurposePhiC31 target with pUBQ10 switching from mTurq to mScarletDepositorInserttRBCS-mScarlet-attP_PhiC31-pUBQ10-attB_PhiC31-mTurq-tUBQ10
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
POC1474
Plasmid#213762PurposeBpiI-associated switch methylaseDepositorInsertBpiI-associated switch methylase M2.NmeMC58II
UseTagsHis6-tagExpressionBacterialMutationPromoterT7Available sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
POC1473
Plasmid#213761PurposeBpiI-associated switch methylaseDepositorInsertBpiI-associated switch methylase M2.NmeMC58II
UseTagsHis6-tagExpressionBacterialMutationPromoterT7Available sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDonor_RB-TnV2
Plasmid#213907PurposeBarcoded mariner transposon with kan selection casette, and modified terminal sequences - barcodes can be added via FseI-SbfI restriction sitesDepositorInsertmariner transposon with kan resistance and FseI-SbfI sites for barcode insertion
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
T1T/pUBQ10(mTurq->mScarlet)
Plasmid#195947PurposeTp901 target with pUBQ10 switching from mTurq to mScarletDepositorInserttRBCS-mScarlet-attP_Tp901-pUBQ10-attB_Tp901-mTurq-tUBQ10
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
T1B/pUBQ10(mTurq->mScarlet)
Plasmid#195946PurposeBxb1 target with pUBQ10 switching from mTurq to mScarletDepositorInserttRBCS-mScarlet-attP_Bxb1-pUBQ10-attB_Bxb1-mTurq-tUBQ10
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTet-IRES-EGFP
Plasmid#64238PurposeLentiviral, doxycycline-inducible system to express transgene of interest and EGFP.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterpTetAvailable sinceJune 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMJA284= pSin-Pur-MCS-Puro
Plasmid#116877PurposeLentiviral transfer vector containing a multible cloning siteDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only