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Plasmid#179269PurposeExpresses GFP-LacI-HIPK2 fusion proteinDepositorInserthomeodomain interacting protein kinase 2 (HIPK2 Human)
UseTagsGFP-LacIExpressionMammalianMutationPromoterCMVAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHBS838 TDP43 RRM-GFP
Plasmid#98249PurposeTDP43 RRM-GFP reporter constructDepositorInsertTARDBP (TARDBP Human)
UseTagsInternal GFP to replace RRM domainsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-dDIO-MCS--4x6T
Plasmid#196422PurposeEmpty vector for astrocyte-selective Dre-dependent AAV expression; astrocyte selectivity generated with 4x6T miRNA targeting cassetteDepositorTypeEmpty backboneUseAAV; Dre/rox; astrocyte-selectiveTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 BATF3
Plasmid#67050PurposeStandard for cell-free expression benchmarking.DepositorInsertBATF3 (BATF3 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHBS1552 H14-SUMO-TDP43 FYW-S-TEV-mCherry
Plasmid#133322PurposeBacterial expression of TDP43 with FYW-S mutant CTDDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionBacterialMutationAll FYW in the TDP43 IDRs mutated to SPromoterAvailable sinceNov. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR357.9
Plasmid#125105PurposeExpression of narL(1-129aa)-ydfI(124-213aa) chimera under PLtetO-1, output Promoter PydfJ115DepositorInsertsnarL(REC)-ydfI(DBD)129
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1413 GFP-TDP43 VLIM-F
Plasmid#118802PurposeTo test the effect of sequence on nuclear body formation and splicing of full-length TDP43DepositorInsertTARDBP mutant (TARDBP Human)
UseLentiviralTagsExpressionMutationAll VLIM to F in CTD of full-length TDP43PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1411 GFP-TDP43 FYW-S
Plasmid#118800PurposeTo test the effect of sequence on nuclear body formation and splicing of full-length TDP43DepositorInsertTARDBP mutant (TARDBP Human)
UseLentiviralTagsExpressionMutationAll FYW to S in CTD of full-length TDP43PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKD279
Plasmid#125099PurposeExpression of SO_4388(1-130aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110DepositorInsertsSO_4388(REC)-psdR(DBD)137
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 EBNA1BP2
Plasmid#67072PurposeStandard for cell-free expression benchmarking.DepositorInsertEBNA1BP2 EBNA1BP2 EBNA1 binding protein 2 (EBNA1BP2 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 GTF2E1
Plasmid#67089PurposeStandard for cell-free expression benchmarking.DepositorInsertGTF2E1 (GTF2E1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHBS1415 GFP-TDP43 VLIM-S
Plasmid#133323PurposeTo test the effect of sequence on nuclear body formation and splicing of full-length TDP43DepositorInsertTARDBP mutant (TARDBP Human)
UseLentiviralTagsExpressionMutationALL VLIM in the TDP43 IDRs mutated to SPromoterAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS953 TARDBP-Exon2-ko-sgRNA2-SpCas9
Plasmid#107858PurposeTo knock-out TDP43DepositorInsertgRNA against TARDBP (Exon 2) (TARDBP Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 FOXM
Plasmid#67118PurposeStandard for cell-free expression benchmarking.DepositorInsertFOXM (FOXM1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSpCAS9n(BB)-2A-puro DNM1, A
Plasmid#107795PurposegRNA vector for knock-in gene editing at human DNM1 locusDepositorInsertgRNA targeting human DNM1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSpCAS9n(BB)-2A-puro DNM1, B
Plasmid#107796PurposegRNA vector for knock-in gene editing at human DNM1 locusDepositorInsertgRNA targeting human DNM1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(-) + SLNCR1 _701-902
Plasmid#86821Purposemammalian expression of lncRNA LINC00673/SLNCR1 deletion constructDepositorInsertLINC00673 (LINC00673 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHBS1412 GFP-TDP43 FYW-L
Plasmid#118801PurposeTo test the effect of sequence on nuclear body formation and splicing of full-length TDP43DepositorInsertTARDBP mutant (TARDBP Human)
UseLentiviralTagsExpressionMutationAll FYW to L in CTD of full-length TDP43PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21 Catalytic Domain of MALT1
Plasmid#48970PurposeExpresses catalytic domain of MALT1 in e.coli AA 329-566DepositorInsertMALT1 (MALT1 Human)
UseTagsHisExpressionBacterialMutationContains AA 329-566PromoterT7Available sinceOct. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Scara3-IRES-BFP
Plasmid#117265PurposeRetroviral overexpression of Scara3DepositorInsertScara3 (Scara3 Mouse)
UseRetroviralTagsExpressionMammalianMutationSee depositor comments below.PromoterLTRAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only