Addgene

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Dox-SOScat

Plasmid

#207329

Purpose

PiggyBac vector backbone encoding doxycycline-inducible expression of membrane-localized catalytic domain of SOS (SOScat)

Depositor

Jared Toettcher

Article

Underhill et al Development. 2023 Aug 15;150(16):dev2016

Insert

son of sevenless homolog 2 variant, catalytic domain

Use

Tags

myristoylation tag - BFP

Expression

Bacterial

Mutation

range: 593 to 1078

Promoter

Tet Response Element

Available since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pMB1-A-ptet-FLAG-dCas9

Plasmid

#190130

Purpose

Level 1 MoClo plasmid containing an aTC inducible FLAG tagged dCas9 for CRISPRi

Depositor

Veronica Godoy-Carter

Article

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Insert

dCas9

Use

CRISPR

Tags

FLAG

Expression

Mutation

Promoter

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pEYFP-N1-HsSmg5_H

Plasmid

#146474

Purpose

Mammalian Expression of HsSmg5

Depositor

Elisa Izaurralde

Article

Unterholzner et al Mol Cell. 2004 Nov 19. 16(4):587-96.

Insert

HsSmg5 (SMG5 Human)

Use

Tags

Expression

Mammalian

Mutation

three non silent mutation A254G (K85R), T980C (L3…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pBBK26 pHomL-TEF2p-Neon(gfp)-Link-GFPdropout-SSA1t-HomR (AmpR)

Plasmid

#179010

Purpose

Parent vector for markerless yeast integration cassettes with N-terminal Neon(gfp)-tagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK25 pHomL-TDH3p-Neon(gfp)-Link-GFPdropout-SSA1t-HomR (AmpR)

Plasmid

#179009

Purpose

Parent vector for markerless yeast integration cassettes with N-terminal Neon(gfp)-tagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK96 pHomL-ConLS-GFPdropout-ConR1-HomR (AmpR)

Plasmid

#179080

Purpose

Parent vector for for creating new markerless yeast integration cassettes with different promoters/genes/tags/terminators

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK42 pHomL-RPL18Bp-GFPdropout-Link-3HA-SSA1t-HomR (AmpR)

Plasmid

#179026

Purpose

Parent vector for markerless yeast integration cassettes with C-terminal 3xHemagglutinin-tagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK41 pHomL-TEF2p-GFPdropout-Link-3HA-SSA1t-HomR (AmpR)

Plasmid

#179025

Purpose

Parent vector for markerless yeast integration cassettes with C-terminal 3xHemagglutinin-tagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK40 pHomL-TDH3p-GFPdropout-Link-3HA-SSA1t-HomR (AmpR)

Plasmid

#179024

Purpose

Parent vector for markerless yeast integration cassettes with C-terminal 3xHemagglutinin-tagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK39 pHomL-RPL18Bp-3HA-Link-GFPdropout-SSA1t-HomR (AmpR)

Plasmid

#179023

Purpose

Parent vector for markerless yeast integration cassettes with N-terminal 3xHemagglutinin-tagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK38 pHomL-TEF2p-3HA-Link-GFPdropout-SSA1t-HomR (AmpR)

Plasmid

#179022

Purpose

Parent vector for markerless yeast integration cassettes with N-terminal 3xHemagglutinin-tagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK37 pHomL-TDH3p-3HA-Link-GFPdropout-SSA1t-HomR (AmpR)

Plasmid

#179021

Purpose

Parent vector for markerless yeast integration cassettes with N-terminal 3xHemagglutinin-tagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK36 pHomL-RPL18Bp-GFPdropout-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

Plasmid

#179020

Purpose

Parent vector for markerless yeast integration cassettes with C-terminal Scarlet(rfp)-tagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK35 pHomL-TEF2p-GFPdropout-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

Plasmid

#179019

Purpose

Parent vector for markerless yeast integration cassettes with C-terminal Scarlet(rfp)-tagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK34 pHomL-TDH3p-GFPdropout-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

Plasmid

#179018

Purpose

Parent vector for markerless yeast integration cassettes with C-terminal Scarlet(rfp)-tagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK33 pHomL-RPL18Bp-Scarlet(rfp)-Link-GFPdropout-SSA1t-HomR (AmpR)

Plasmid

#179017

Purpose

Parent vector for markerless yeast integration cassettes with N-terminal Scarlet(rfp)-tagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK32 pHomL-TEF2p-Scarlet(rfp)-Link-GFPdropout-SSA1t-HomR (AmpR)

Plasmid

#179016

Purpose

Parent vector for markerless yeast integration cassettes with N-terminal Scarlet(rfp)-tagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK30 pHomL-RPL18Bp-GFPdropout-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179014

Purpose

Parent vector for markerless yeast integration cassettes with C-terminal Neon(gfp)-tagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK29 pHomL-TEF2p-GFPdropout-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179013

Purpose

Parent vector for markerless yeast integration cassettes with C-terminal Neon(gfp)-tagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK27 pHomL-RPL18Bp-Neon(gfp)-Link-GFPdropout-SSA1t-HomR (AmpR)

Plasmid

#179011

Purpose

Parent vector for markerless yeast integration cassettes with N-terminal Neon(gfp)-tagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge