159,117 results

1. pSL1-GFP

   Plasmid

   #180420

   Purpose

   Kan resistant plasmid derived from pBTK402, a broad-host-range plasmid previously shown to replicate in multiple species of bee gut bacteria. It encodes a GFP promoter.

   Depositor

   Nancy Moran

   Article

   Li et al Proc Natl Acad Sci U S A. 2022 May 3;119

   Insert

   GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pT7-PE7 for IVT

   Plasmid

   #214813

   Purpose

   Template for in vitro transcription of PE7

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7
   

   Use

   Template for in vitro transcription

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mutation

   Promoter

   T7 (inactivated)

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PB-TO-oNGN2

   Plasmid

   #198397

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase, NGN2 optimization aided by IDT tool.

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Inserts

   NEUROG2 (phospho mutant, codon optimized) (NEUROG2 Human)
   puroR-T2A-mycNLS-mTagBFP2
   rtTA3G
   

   Use

   Tags

   T2A-mycNLS-mTagBFP2

   Expression

   Mammalian

   Mutation

   S24A, S193A, S207A, S209A, S219A, S232A, S239A, S…

   Promoter

   CAG, EF1a, and TRE3G

   Available since

   April 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Cbh_v5 AAV-ABE N-terminal

   Plasmid

   #137177

   Purpose

   AAV genome: expresses the N-terminal of v5 AAV-ABE from the Cbh promoter

   Depositor

   David Liu

   Article

   Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

   Insert

   v5 AAV-ABE N-terminal
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Cas9 D10A

   Promoter

   Cbh

   Available since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDONR221_SLC1A4

   Plasmid

   #131962

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC1A4 (SLC1A4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 21, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. 3XFlag-pA-Tn5-Fl

   Plasmid

   #124601

   Purpose

   Expresses protein A and Tn5 Transposase fusion protein in bacterial cells

   Depositor

   Steven Henikoff

   Article

   Kaya-Okur et al Nat Commun. 2019 Apr 29;10(1):1930. doi:

   Insert

   Protein A and hyperactive Tn5 transposase (Tnp) fusion protein
   

   Use

   Tags

   Protein A and Tn5 transposase fusion protein with…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. SpyCatcher003-mi3

   Plasmid

   #159995

   Purpose

   Expresses SpyCatcher003-mi3 in bacterial cells as virus-like particle platform for plug-and-display vaccination

   Depositor

   Mark Howarth

   Article

   Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

   Insert

   SpyCatcher003-mi3
   

   Use

   Tags

   C-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDONR221_SLC6A19

   Plasmid

   #132231

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC6A19 (SLC6A19 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 8, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. mCherry-GAL9

   Plasmid

   #166689

   Purpose

   For creating a stable mCherry-GAL9 cell line

   Depositor

   Alan Sabirsh

   Article

   Munson et al Commun Biol. 2021 Feb 16;4(1):211. doi:

   Insert

   LGALS9 (LGALS9 Human)
   

   Use

   Synthetic Biology

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBS1C

    Plasmid

    #55168

    Purpose

    Empty vector, integration at amyE, ampr, cmr

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Type

    Empty backbone

    Use

    Synthetic Biology; Bacillus biobrick box

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pXPR_502

    Plasmid

    #96923

    Purpose

    for CRISPRa, lentiviral expression of gRNA scaffold using activator P65 HSF

    Depositor

    David Root, John Doench

    Article

    Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDONR221_SLC1A2

    Plasmid

    #131901

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC1A2 (SLC1A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pDONR221_SLC6A15

    Plasmid

    #131877

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC6A15 (SLC6A15 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 9, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pDONR221_SLC38A3

    Plasmid

    #132142

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC38A3 (SLC38A3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 25, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pDONR221_SLC7A10

    Plasmid

    #132232

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC7A10 (SLC7A10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pDONR221_SLC38A8

    Plasmid

    #132082

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC38A8 (SLC38A8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pPath-Violacein-native

    Plasmid

    #200001

    Purpose

    Violacein pathway. Native promoter and CDS sequence

    Depositor

    Farren Isaacs

    Article

    Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

    Insert

    Pathways
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1-Flag

    Plasmid

    #208051

    Purpose

    Empty vector for mammalian expression, insert a Flag tag (with ATG) at XhoI site of pcDNA3.1 (-), xhoI site at 5' of flag tag destroyed, xhoI site at 3' of flag tag retained. XhoI in-frame with Flag

    Depositor

    Quanfu Ma

    Type

    Empty backbone

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-Bxb1

    Plasmid

    #182142

    Purpose

    Expression of human codon optimized Bxb1

    Depositor

    David Liu

    Article

    Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

    Insert

    human codon optimized Bxb1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET15b-RvLEAMshort

    Plasmid

    #218122

    Purpose

    Expresses truncated RvLEAM protein under IPTG induction in bacterial host cells.

    Depositor

    Ci Ji Lim

    Article

    Abe et al bioRxiv [Preprint]. 2024 Feb 11:2024.02.

    Insert

    RvLEAMshort
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Truncated form (58-181aa)

    Promoter

    T7

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge