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Plasmid#65448PurposeExpression of RFP in mammalian cells. Control vector for FUW-Ephrin-RFP vectorsDepositorInsertSV40-RFP
UseLentiviralTagsExpressionMammalianMutationPromoterUBQAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
attB-GFP-Poly(A)‐NeoR
Plasmid#65526PurposeNeoR containing plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.DepositorInsertattB-GFP
UseMouse Targeting; Bxb1TagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-p97-EQ-mycStrep
Plasmid#31838DepositorInsertp97
UseTagsStrep and mycExpressionBacterial and MammalianMutationE578QPromoterCMVAvailable sinceOct. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
L309-myc-seipin-WT
Plasmid#64061Purposemyc-tagged murine seipin (wild type); lentiviral constructDepositorInsertbscl2 (Bscl2 Mouse)
UseLentiviralTagsmyc epitopeExpressionMammalianMutationPromoterAvailable sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
promoter J23110 (upstream of RBS, mRFP1)
Plasmid#117852PurposeFor course in "Synthetic Biology: A Lab Manual"DepositorInsertmedium-high promoter, RBS, mRFP1
UseSynthetic Biology; Escherichia coliTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
L-CRISPR-CTN (MLL-ctrl)
Plasmid#69215PurposeAdvanced lentiviral CRISPR-Cas9 vector for induction of chromosomal translocations; control, MLL-sgRNA + luciferase-sgRNADepositorInsertsSpCas9
Sp sgRNA scaffold
SFFV promoter
P2A-mNeonGreen
UseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-p97wt-mycStrep
Plasmid#31837DepositorInsertp97
UseTagsStrep and mycExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceOct. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1 puro-luc (shRNA)
Plasmid#153962PurposeExpresses a shRNA against firefly luciferaseDepositorInsertshRNA against firefly luciferase (19-bp-long target)
UseLuciferase, RNAi, and RetroviralTagsN/AExpressionMammalianMutationPromoterU6 promoterAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
SpCas9 TadCBEb
Plasmid#193832PurposeExpress TadCBEb (with SpCas9) in mammalian cellsDepositorInsertTadA-CDb-SpCas9 D10A-2xUGI
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSN25
Plasmid#100110Purposeexpresses a chimeric SOX2-repressor fusionDepositorUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pv6_TAF3_2xPhd
Plasmid#179399PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of TAF3_2xPhdDepositorInsertTAF3_2xPhd
UseTagsAviTag and EGFPExpressionMammalianMutationPromoterCAGGSAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFUW-TetO-MXD1
Plasmid#178443Purposedoxycycline-inducible expression of Human MXD1 in mammalian cellsDepositorInsertMXD1 (MXD1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
SpCas9 TadCBEe
Plasmid#193836PurposeExpress TadCBEe (with SpCas9) in mammalian cellsDepositorInsertTadA-CDe-SpCas9D10A-2xUGI
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUDE735
Plasmid#103024Purposeexpression of a Cpf1 programming crRNA targetting CAN1, HIS4, PDR12 and ADE2 (crCAN1-4.crHIS4-4.crPDR12-3.crADE2-3.S)DepositorInsertcrCAN1-4.crHIS4-4.crPDR12-3.crADE2-3.S
UseCRISPRTagsExpressionYeastMutationPromoterSNR52Available sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGST2-GST-TEV-OPTN
Plasmid#198428PurposeUsed for the expression and purification of OPTN (WT) (SMC Internal No. 1587)DepositorInsertoptineurin (OPTN Human)
UseTagsGST and TEVExpressionBacterialMutationPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-mGold-Lysosome-N-20
Plasmid#158006PurposeVisualization of lysosome in mammalian cells using mGoldDepositorInsertLAMP1 (Lamp1 Rat)
UseTagsmGoldExpressionMammalianMutationPromoterCMVAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pyc128m
Plasmid#158778Purposesoluble GCaMP expressionDepositorInsertGCaMP6m
UseAAVTagsExpressionMutationPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIRESpuro2-UBXD1-mCherry
Plasmid#31835DepositorInsertUBXD1 (UBXN6 Human)
UseTagsmCherryExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceOct. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV iGluf
Plasmid#105833PurposeFast indicator for extracellular glutamate imagingDepositorInsertiGluSnFR E25D variant
UseTagsMyc and PDGFRExpressionMammalianMutationChanged Glu 25 to AspPromoterCMVAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pv6_CBX1_2xChromo
Plasmid#179393PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1_2xChromoDepositorInsertCBX1_2xChromo
UseTagsAviTag and EGFPExpressionMammalianMutationPromoterCAGGSAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only