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Plasmid#210612PurposeLentiviral vector expressing gRNA targeting a control region at the CXCR4 locusDepositorInsertControl(1)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_mpx_AsCas12a_h7SK(PacI)_U6(I-SceI)_PGK-puro
Plasmid#189633PurposeLentiviral expression of dual AsCas12a gRNAs for generating combinatorial AsCas12a 3Cs librariesDepositorInserth7SK Cas12a gRNA cassette, hU6 Cas12a gRNA cassette, PGK puro cassette
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(RIC8A(16))-PGKpuro2ABFP-W
Plasmid#200491PurposeLentiviral vector expressing gRNA targeting human RIC8ADepositorInsertRIC8A(16) (RIC8A Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(RIC8A(15))-PGKpuro2ABFP-W
Plasmid#200490PurposeLentiviral vector expressing gRNA targeting human RIC8ADepositorInsertRIC8A(15) (RIC8A Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Control(2))-PGKpuro2AmCherry-W
Plasmid#210613PurposeLentiviral vector expressing gRNA targeting a control region at the CXCR4 locusDepositorInsertControl(2)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMtnA FLAG-IntS7 puro
Plasmid#208405PurposeExpresses FLAG-tagged Drosophila IntS7 from inducible MtnA promoterDepositorInsertpMT FLAG MCS puro (defl Fly)
UseTags3xFLAGExpressionInsectMutationPromoterMtnAAvailable sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMtnA FLAG-IntS13 puro
Plasmid#208406PurposeExpresses FLAG-tagged Drosophila IntS13 from inducible MtnA promoterDepositorInsertIntS13 (asun Fly)
UseTags3xFLAGExpressionInsectMutationPromoterMtnAAvailable sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMtnA FLAG-IntS14 puro
Plasmid#208407PurposeExpresses FLAG-tagged Drosophila IntS14 from inducible MtnA promoterDepositorInsertIntS14 (IntS14 Fly)
UseTags3xFLAGExpressionInsectMutationPromoterMtnAAvailable sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
LENTICRISPR-LUC7L2_sgRNA1
Plasmid#201602PurposeCRISPR/Cas9-mediated gene knock-outDepositorInsertLUC7L2 (LUC7L2 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-moxGFP-Puro-LAMP1
Plasmid#207788PurposeDonor template for moxGFP-2A-Puro insertion into the C-terminus of the LAMP1 locus. For lysosome visualization.To be co-transfected with sgRNA plasmid px330-LAMP1 (Addgene #207787)DepositorInsertLAMP1 Homology Arms flanking a moxGFP-Puro Cassette (LAMP1 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceDec. 1, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMtnA FLAG-IntS6 puro
Plasmid#195076PurposeExpresses FLAG-tagged Drosophila IntS6 from inducible MtnA promoterDepositorInsertIntS6 (IntS6 Fly)
UseTags3xFLAGExpressionInsectMutationPromoterMtnAAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMtnA FLAG-IntS12 puro
Plasmid#195077PurposeExpresses FLAG-tagged Drosophila IntS12 from inducible MtnA promoterDepositorInsertIntegrator 12 (IntS12 Fly)
UseTags3xFLAGExpressionInsectMutationPromoterMtnAAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1003
Plasmid#143089PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZNF300 (ZNF300 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceNov. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CHyMErA.v2_U6(SpCas9_I-SceI)-(AsCas12a(dual-gRNA)_PacI-PacI)_PGK-puro
Plasmid#189636PurposeLentiviral expression of single SpCas9 and dual AsCas12a gRNAs for generating combinatorial CHyMErA.v2 3Cs librariesDepositorInserthU6 Cas9-Cas12a-Cas12a gRNA cassette, PGK puro cassette
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti_mpx_SpCas9_h7SK(PacI)_U6(I-SceI)_PGK-puro_v2
Plasmid#189632PurposeLentiviral expression of dual SpCas9 gRNAs for generating combinatorial SpCas9 3Cs librariesDepositorInserth7SK Cas9 gRNA cassette, hU6 Cas9 gRNA cassette, PGK puro cassette
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Puro-mNeon-ACTB
Plasmid#207752PurposeDonor template for Puro-2A-mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748DepositorInsertACTB Homology Arms flanking a Puro-mNeon Cassette (ACTB Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Puro-moxGFP-TUBA1B
Plasmid#207767PurposeDonor template for Puro-2A-moxGFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763DepositorInsertTUBA1B Homology Arms flanking a Puro-moxGFP Cassette (TUBA1B Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-N-Puro-sTagRFP-TUBA1B
Plasmid#207769PurposeDonor template for Puro-2A-sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763DepositorInsertTUBA1B Homology Arms flanking a Puro-sTagRFP Cassette (TUBA1B Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
TFORF0231
Plasmid#143207PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZNF595 (ZNF595 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV_dCas9-KRAB_sgSTK4i_#1 (PuroR)
Plasmid#209773PurposeCRISPRi for STK4DepositorInsertdCas9-KRAB, sgSTK4i (STK4 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only