qTAG-N-Puro-sTagRFP-TUBA1B
(Plasmid #207769)

• Purpose: Donor template for Puro-2A-sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763
• Depositing Lab: Laurence Pelletier
• Publication: Philip et al bioRxiv

Backbone

• Vector backbone: pUC19
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2686
• Vector type: Mammalian Expression, CRISPR ; Donor Template
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TUBA1B Homology Arms flanking a Puro-sTagRFP Cassette
• Alt name: TUBA1B
• Species: H. sapiens (human)
• Insert Size (bp): 2712
• Entrez Gene: TUBA1B (a.k.a. K-ALPHA-1)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CTGCAAGGCGATTAAGTTGGGTAAC
• 3′ sequencing primer: GGCTCGTATGTTGTGTGGAATTGT

Resource Information

• Supplemental Documents:
  • qTAG-N-Puro-sTagRFP-TUBA1B.dna
  • 207769-TUBA1B-Genomic-Design.dna
  • 207769-TUBA1B-LHA.dna
  • 207769-TUBA1B-RHA.dna

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B https://www.addgene.org/207763 .