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Plasmid#89448PurposeExpression of GFP-tagged human Rab27B Q78L in mammalian cellsDepositorInserthuman Rab27B Q78L (RAB27B Human)
UseTagseGFPExpressionMammalianMutationQ78LPromoterCMVAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNL-CEF-BAFFR-CFP
Plasmid#187002PurposeExpression of human BAFFR-CFP fusion proteinDepositorInsertBAFFR (TNFRSF13C Human)
UseLentiviralTagsCFPExpressionMutationPromoterAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLEVI(408)-ColE-iLight-msfGFP
Plasmid#170268PurposeExpresses LexA_DBD-iLight-msfGFP under J23116 promoter and mCherry under ColE promoter with a LexA408 operator in bacteria.DepositorInsertiLight
UseTagsmsfGFPExpressionBacterialMutationTo design iLight for expression in bacteria, a fu…PromoterpJ23116Available sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Con/Foff 2.0-ChRmine-oScarlet
Plasmid#137161PurposeIntersectional viral expression of ChRmine-p2a-oScarlet in cells expressing Cre AND NOT FlpDepositorHas ServiceAAV8InsertCon/Foff 2.0-ChRmine-p2a-oScarlet
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationoScarlet E95DPromoternEFAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCW57-Cx46-IRES-GCaMP6s
Plasmid#188238Purpose3rd generation, inducible bicistronic lentiviral plasmid for expression of human connexin 46 and GCaMP6sDepositorInsertGJA3 (GJA3 Human)
UseLentiviralTagsGCaMp6sExpressionMammalianMutationPromoterTight TRE promoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
G protein alpha-s(alpha3beta5/G226A/A366S)
Plasmid#55797PurposeThis highly effective dominant negative G protein alpha-s mutant contains, in addition to the mutations in alpha-s(alpha3beta5/G226A), the A366S mutation, which increases GDP release.DepositorInsertalpha-s (alpha3beta5/G226A/A366S) (Gnas Rat)
UseTagsinternal EE epitope (residues 189-194 in alpha-s …ExpressionMammalianMutationN271K, K274D, R280K, T284D, I285T, G226A, A366S i…PromoterCMVAvailable sinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-fDIO-Cre
Plasmid#121675PurposeExpresses Cre recombinase and HA tag in a Flp dependent fashion (fDIO)DepositorHas ServiceAAV Retrograde, AAV1, AAV5, AAV8, and AAV9InsertNLS-CRE-HA
UseAAVTags3xHA and SV40-NLSExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLPB3B-AID-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin-T2A-osTIR1
Plasmid#187960PurposeAID degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance, T2A site and osTIR1 under PGK promoter.DepositorInsertAID-SpdCas9-tagRFPt-P2A-tagBFP; Blasticidin-T2A-osTIR1
UseCRISPR and Synthetic BiologyTagsGGS linkerExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Fon-mCherry
Plasmid#137132PurposeIntersectional viral expression of mCherry in cells expressing both Cre AND FlpDepositorHas ServiceAAV8InsertCon/Fon-mCherry
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationn/aPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
P-Ef1a-RPL22-3XHA-P2A-mCherry-T2A-Puro
Plasmid#170320PurposeExpresses HA and mCherry tagged RPL22, puromycin resistanceDepositorInsertRPL22 (RPL22 Human)
UseLentiviralTagsExpressionMutationNonePromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
P-Ef1a-RPL22-3XHA-P2A-EGFP-T2A-Puro
Plasmid#170317PurposeExpresses HA and EGFP tagged RPL22, puromycin resistanceDepositorInsertRPL22 (RPL22 Human)
UseLentiviralTagsExpressionMutationNonePromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-FLAG-SRSF3_Full.Length_Codon.Optimized-Blast
Plasmid#171951PurposeAll-in-one lentiviral vector for doxycycline-inducible expression of FLAG-tagged, codon-optimized, full-length SRSF3DepositorInsertserine and arginine rich splicing factor 3 (SRSF3 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationcodon-optimizedPromoterTREAvailable sinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLAG-puro-PTPIP51(1-150_175-470)
Plasmid#170537PurposeExpresses PTPIP51 FFAT-like motif (151-175) deletion mutant (1-150_176-470) in human HeLa cells after transfectionDepositorInsertProtein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
UseTagsFLAG tagExpressionMammalianMutationdeleted amino acids 151-175PromoterCAG promoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
hyBE4max
Plasmid#157942PurposeMammalian expression of hyBE4maxDepositorInserthyBE4max
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
EF.delmFasL.CMV.GFP
Plasmid#17620DepositorInsertdelFasL (Fasl Mouse)
UseLentiviralTagsExpressionMammalianMutationNoncleavable mouse delFasL . Deleted the exon 2 r…PromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Con/Foff 2.0-iC++-EYFP
Plasmid#137156PurposeIntersectional viral expression of ic++-EYFP in cells expressing Cre AND NOT FlpDepositorHas ServiceAAV8InsertCon/Foff 2.0-iC++-EYFP
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationn/aPromoternEFAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
R619-M66-303: CMV51p> HCoV-OC43 S-2P-T4f-3C-His8-Strep2x2
Plasmid#166015Purposemammalian expression of HCoV-OC43 soluble spike trimer proteinDepositorInsertHCoV-OC43 S (spike)
UseTags3C-His8-Strep2x2ExpressionMammalianMutationPromoterCMV51Available sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
R619-M89-303: CMV51p> HCoV-HKU1 S-2P-T4f-3C-His8-Strep2x2
Plasmid#166014Purposemammalian expression of HCoV-HKU1 soluble spike trimer proteinDepositorInsertHCoV-HKU1 S (spike)
UseTags3C-His8-Strep2x2ExpressionMammalianMutationPromoterCMV51Available sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
CARPID dCasRx-BASU
Plasmid#153303Purposeexpress CARPID dCasRx-BASU fusion protein in mammalian cellsDepositorInsertdCasRx-BASU
UseCRISPR and LentiviralTagsExpressionMammalianMutationR239A/H244A/R858A/H863A in RfxCas13dPromoterEF-1aAvailable sinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
P-rTetO-EGFP-T2A-RPL22-3xHA
Plasmid#170326PurposeTetracycline-inducible expression of HA and EGFP tagged RPL22DepositorInsertRPL22 (RPL22 Human)
UseLentiviralTagsExpressionMutationNonePromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only