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Plasmid#160990PurposeExpress a fragment of yeast pol1 proteinDepositorInsertpol1
UseTagsGSTExpressionBacterialMutationF58A/D62APromoterAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPEE45
Plasmid#139424PurposeRecyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mTurquoise2 ORF and a flexible linker at the 5' end.DepositorInsertmTurquoise1
UseTagsExpressionYeastMutationPromoterTEF1Available sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPEE44
Plasmid#139423PurposeRecyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mTFP1 ORF and a flexible linker at the 5' end.DepositorInsertmTFP1
UseTagsExpressionYeastMutationPromoterTEF1Available sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPEE43
Plasmid#139422PurposeRecyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mMaroon1 ORF and a flexible linker at the 5' end.DepositorInsertmMaroon1
UseTagsExpressionYeastMutationPromoterTEF1Available sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGWKS17
Plasmid#139420PurposeFluoroescent marker for use in Cryptococcus neoformans. Contains a non-codon optimised mCherry marker.DepositorInsertmCherry
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-ATG18
Plasmid#109504PurposeMuscle specific zebrafish expression of mKate2 fused to yeast ATG18 (PLEKHA3). Binds PtdIns(3,5P)2DepositorInsertactc1b-mKate2-ATG18
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAWH-IRP
Plasmid#133896PurposePositive control when used with pMS22H-IRE. Expression of Gal4AD-IRP hybrid protein. Homology regions for recombination with pMS22HDepositorInsertIRP
UseTagsExpressionYeastMutationPromoterT7Available sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA0057
Plasmid#131784PurposeGuide RNA (gGFP) and Cas9 expression plasmid for cleaving pFA6 series GFP C-terminal tagging cassettes, including GFP-His3MX6. Used to perform CRISPR-Swap of alleles.DepositorInsert20mer GFP cassette guide (gGFP) and 5' sgRNA
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTJK513
Plasmid#121470PurposeCEN LEU2 CNB1mutEF1DepositorInsertCNB1mutEF1 (CNB1 Budding Yeast)
UseYeast cen plasmidTagsExpressionMutationChanged aspartic acid 34 to alaninePromoterEndogenous genomic promoter regionAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTJK511
Plasmid#121468Purpose2µm HIS3 CNB1mutEF4DepositorInsertCNB1mutEF4 (CNB1 Budding Yeast)
UseYeast high copy 2 micron vectorTagsExpressionMutationChanged aspartic acid 144 to alaninePromoterEndogenous genomic promoter regionAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTJK510
Plasmid#121465Purpose2µm HIS3 CNB1mutEF3DepositorInsertCNB1mutEF3 (CNB1 Budding Yeast)
UseYeast high copy 2 micron vectorTagsExpressionMutationChanged aspartic acid 103 to alaninePromoterEndogenous genomic promoter regionAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTJK509
Plasmid#121464Purpose2µm HIS3 CNB1mutEF2DepositorInsertCNB1mutEF2 (CNB1 Budding Yeast)
UseYeast high copy 2 micron vectorTagsExpressionMutationChanged aspartic acid 66 to alaninePromoterEndogenous genomic promoter regionAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTJK508
Plasmid#121463Purpose2µm HIS3 CNB1mutEF1DepositorInsertCNB1mutEF1 (CNB1 Budding Yeast)
UseYeast high copy 2 micron vectorTagsExpressionMutationChanged aspartic acid 34 to alaninePromoterEndogenous genomic promoter regionAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTJK516
Plasmid#121473PurposeCEN LEU2 CNB1mutEF4DepositorInsertCNB1mutEF4 (CNB1 Budding Yeast)
UseYeast cen plasmidTagsExpressionMutationChanged aspartic acid 144 to alaninePromoterEndogenous genomic promoter regionAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTJK506
Plasmid#121461PurposeCEN HIS3 CNB1mutEF4DepositorInsertCNB1mutEF4 (CNB1 Budding Yeast)
UseYeast cen plasmidTagsExpressionMutationChanged aspartic acid 144 to alaninePromoterEndogenous genomic promoter regionAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTJK505
Plasmid#121460PurposeCEN HIS3 CNB1mutEF3DepositorInsertCNB1mutEF2 (CNB1 Budding Yeast)
UseYeast cen plasmidTagsExpressionMutationChanged aspartic acid 103 to alaninePromoterEndogenous genomic promoter regionAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTJK504
Plasmid#121459PurposeCEN HIS3 CNB1mutEF2DepositorInsertCNB1mutEF2 (CNB1 Budding Yeast)
UseYeast cen plasmidTagsExpressionMutationChanged aspartic acid 66 to alaninePromoterEndogenous genomic promoter regionAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTJK503
Plasmid#121458PurposeCEN HIS3 CNB1mutEF1DepositorInsertCNB1mutEF1 (CNB1 Budding Yeast)
UseYeast cen plasmidTagsExpressionMutationChanged aspartic acid 34 to alaninePromoterEndogenous genomic promoter regionAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS-ATG1-ATG7 3'UTR(406)
Plasmid#122070PurposeExpressing ATG1 with ATG7 3'UTRDepositorInsertserine/threonine protein kinase ATG1 (ATG1 Budding Yeast)
UseTagsExpressionYeastMutationThe native 3'UTR was replaced by ATG7 3'…PromoterAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCu-GFP-Atg17(416)
Plasmid#121408PurposeExpressing GFP-Atg17DepositorInsertCUP1p-GFP-ATG17 ORF-CYC1 terminator
UseTagsExpressionYeastMutationPromoterCUP1 promoterAvailable sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only