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Plasmid#188072PurposeMammalian cell expression of truncated version (aa 1-560) of rat kinesin-1 KIF5C tagged with Halo and Flag tags. The delta6 mutation removes the first 6 amino acids.DepositorInsertrat KIF5C (Kif5c Rat)
UseTagsFlag and HaloExpressionMammalianMutationaa1-560, deletion of aa2-6PromoterCMVAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMega-MaFRSA
Plasmid#200226PurposeThe plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with meta-trifluoromethylphenylalanine..DepositorInsertsPyrrolysyl-tRNA synthetase
pyrrolysyl-tRNA
UseTagsnoneExpressionBacterialMutationmutated Asparagine 166 to Alanine and Valine 168 …PromoterproK-lacO and tacIAvailable sinceJune 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMH003_phiC31_neo-5xTetO-pEF-H2B-mCitrine-coreHS4-pRSV-H2B-mCherry
Plasmid#179431PurposeDual fluorescent reporter construct with single core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integrationDepositorInsertTRE-cit-coreHS4-mch (SC)
UseTagsExpressionMammalianMutationPromoterpEF alpha, pRSVAvailable sinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
24xMoonTag-kif18b-STOP-24xSunTag-linker-24xPP7 (3’UTR translation reporter)
Plasmid#128605PurposeExpresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene, STOP codon, 24xgcn4 peptide array (SunTag peptide array), linker and 24 repeats of PP7 hairpins.DepositorInsertsUseTagsExpressionMammalianMutationPromoterPcmvAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX301
Plasmid#25895DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Gateway destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Coff/Fon-ChRmine-oScarlet
Plasmid#137160PurposeIntersectional viral expression of ChRmine-p2a-oScarlet in cells expressing Flp AND NOT CreDepositorHas ServiceAAV8InsertCoff/Fon-ChRmine-p2a-oScarlet
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationoScarlet E95DPromoternEFAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
FKBP:FRB-FBAR
Plasmid#223620PurposeTransient expression of human FBP17 with mGFP, FRB, FKBP and TagBFP tags in mammalian cellsDepositorInsertFBP17 (FNBP1 Human)
UseTagsFKBP, TagBFP and mGFP, FRBExpressionMammalianMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-Rab27A
Plasmid#89237PurposeExpression of GFP-tagged human Rab27A in mammalian cellsDepositorInserthuman Rab27A (RAB7A Human)
UseTagseGFPExpressionMammalianMutationwild typePromoterCMVAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLX302
Plasmid#25896DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Gateway destination vectorTagsV5ExpressionMammalianMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Tox-IRES-eGFP
Plasmid#165427PurposeExpresses TOX in mammalian cellsDepositorInsertTOX (Tox Mouse)
UseTags3xflag 5' of ToxExpressionMammalianMutationPromoter5' LTRAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
hyA3A-BE4max
Plasmid#157943PurposeMammalian expression of hyA3A-BE4maxDepositorInserthyA3A-BE4max
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIR-CMV-SCN1A-Variant-1-IRES-mScarlet
Plasmid#162278PurposeEukaryotic expression of human SCN1A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.DepositorInsertSCN1A Variant 1, stabilized (SCN1A Human)
UseTagsIRES-mScarletExpressionMammalianMutationIVS intron inserted after bp2946. Silent codon c…PromoterCMVAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV_UdgX-APOBEC1-UdgX-NG-nCas9
Plasmid#163562PurposeMammalian CG-to-GC base editingDepositorInsertUdgX-APOBEC1-UdgX-NG-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…PromoterAvailable sinceDec. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Coff/Fon-BFP
Plasmid#137131PurposeIntersectional viral expression of BFP in cells expressing Flp AND NOT CreDepositorHas ServiceAAV8InsertCoff/Fon-BFP
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationn/aPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
hyeA3A-BE4max
Plasmid#157944PurposeMammalian expression of hyeA3A-BE4maxDepositorInserthyeA3A-BE4max
UseCRISPRTagsExpressionMammalianMutationN57GPromoterAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Cas9-T2A-TdT
Plasmid#126424PurposeExpresses Cas9-T2A-TdT in mammalian cells. (pTBL209)DepositorInsertCas9-T2A-TdT (DNTT Human, Streptococcus pyogenes)
UseTagsExpressionMammalianMutationPromoterpCMVAvailable sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
MYH11-NanoLuc-2A-tdTomato
Plasmid#132569PurposeDonor vector to knockin NanoLuc and tdTomado (separated by 2A) into MYH11 alleleDepositorInsertHuman MYH11 knockin homology arms (MYH11 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Con/Fon-ChRmine-oScarlet
Plasmid#137159PurposeIntersectional viral expression of ChRmine-p2a-oScarlet in cells expressing both Cre AND FlpDepositorHas ServiceAAV8InsertCon/Fon-ChRmine-p2a-oScarlet
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationoScarlet E95DPromoternEFAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_CDC50A
Plasmid#203694Purposeexpresses CDC50ADepositorInsertTMEM30A (TMEM30A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-Con/Fon-GCaMP6M
Plasmid#137119PurposeIntersectional viral expression of GCaMP6M in cells expressing both Cre AND FlpDepositorHas ServiceAAV8InsertCon/Fon-GCaMP6M
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationRemoved RSET tagPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only