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pDONR223_PIK3IP1_p.G185E

Plasmid

#81339

Purpose

Gateway Donor vector containing PIK3IP1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PIK3IP1 (PIK3IP1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

G185E; T251S

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RBM10_p.R171W

Plasmid

#81592

Purpose

Gateway Donor vector containing RBM10 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RBM10 (RBM10 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

R171W

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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DCLK2 gRNA (BRDN0001162199)

Plasmid

#75701

Purpose

3rd generation lentiviral gRNA plasmid targeting human DCLK2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DCLK2 (DCLK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pPICZ:K2P2.1(mTREK-1)Cryst S112C

Plasmid

#224780

Purpose

Pichia pastoris expression vector for generating mouse TREK-1(21S-322T) + 3C cleavable site + eGFP + 10x His under the AOX1 promoter

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

Tags

Expression

Yeast

Mutation

K84R / Q85E / T86K / I88L / A89R / Q90A / A92P / …

Promoter

AOX1 promoter

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-FLAG-msMMS22L

Plasmid

#221015

Purpose

mammalian expression vector of Flag tagged mouse MMS22L

Depositor

Daniel Durocher

Article

O'Donnell et al Mol Cell. 2010 Nov 24;40(4):619-31. doi:

Insert

MMS22L (Mms22l Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

naturally siResistant to siMMS22L (human) aagacuu…

Promoter

CMV

Available since

Sept. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT/TO-eGFP-msMMS22L

Plasmid

#221014

Purpose

mammalian expression vector of eGFP tagged mouse MMS22L

Depositor

Daniel Durocher

Article

O'Donnell et al Mol Cell. 2010 Nov 24;40(4):619-31. doi:

Insert

MMS22L (Mms22l Mouse)

Use

Tags

eGFP

Expression

Mammalian

Mutation

naturally siResistant to siMMS22L (human) aagacuu…

Promoter

CMV

Available since

Sept. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBK1303-AAV-EFSNC-dCjCas9-MECP2(204-310)

Plasmid

#223148

Purpose

Expression of truncated MECP2 with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

MECP2 (MECP2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 204-310

Promoter

EF1a

Available since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1297-AAV-EFSNC-dCjCas9-HP1b(79-173)

Plasmid

#223142

Purpose

Expression of truncated HP1b with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

HP1b (CBX1 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 79-173

Promoter

EF1a

Available since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1295-AAV-EFSNC-dCjCas9-HP1a(72-177)

Plasmid

#223140

Purpose

Expression of truncated HP1a with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

HP1a (CBX5 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 72-177

Promoter

EF1a

Available since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1305-AAV-EFSNC-dSaCas9-HP1a(72-177)

Plasmid

#223150

Purpose

Expression of truncated HP1a with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

HP1a (CBX5 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 72-177

Promoter

EF1a

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1306-AAV-EFSNC-dSaCas9-HP1aNH(115-177)

Plasmid

#223151

Purpose

Expression of truncated HP1a with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

HP1a (CBX5 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 115-177

Promoter

EF1a

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1296-AAV-EFSNC-dCjCas9-HP1aNH(115-177)

Plasmid

#223141

Purpose

Expression of truncated HP1a with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

HP1a (CBX5 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 115-177

Promoter

EF1a

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-GFP-LA

Plasmid

#206197

Purpose

Expresses GFP-tagged human Lamin-A protein by the constitutive CMV promoter in mice

Depositor

Yuxuan Guo

Article

Sun et al J Am Coll Cardiol Basic Trans Science. n

Insert

Lmna (LMNA )

Use

AAV

Tags

GFP

Expression

Mutation

Promoter

CMV

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Tnnt2-GFP-LA

Plasmid

#206198

Purpose

Expresses GFP-tagged Lamin-A specifically in cardiomyocytes by the Tnnt2 promoter

Depositor

Yuxuan Guo

Article

Sun et al J Am Coll Cardiol Basic Trans Science. n

Insert

Lmna (LMNA Human)

Use

AAV

Tags

GFP

Expression

Mutation

Promoter

Tnnt2

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1304-AAV-EFSNC-dCjCas9-KRAB-MECP2

Plasmid

#223149

Purpose

Expression of KRAB and truncated MECP2 with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

KRAB-MECP2 (MECP2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 204-310 (MECP2)

Promoter

EF1a

Available since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1314-AAV-EFSNC-dSaCas9-KRAB-MECP2

Plasmid

#223159

Purpose

Expression of KRAB and truncated MECP2 with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

KRAB-MECP2 (MECP2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 204-310 (MECP2)

Promoter

EF1a

Available since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1313-AAV-EFSNC-dSaCas9-MECP2(204-310)

Plasmid

#223158

Purpose

Expression of truncated MECP2 with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

MECP2 (MECP2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 204-310

Promoter

EF1a

Available since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1307-AAV-EFSNC-dSaCas9-HP1b(79-173)

Plasmid

#223152

Purpose

Expression of truncated HP1b with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

HP1b (CBX1 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 79-173

Promoter

EF1a

Available since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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JO-pH720-SARS-CoV-2-S-tail-Cys1254Ala-Thr1273Glu

Plasmid

#218454

Purpose

Bacterial expression plasmid for the cytosolic tail of SARS-CoV-2 spike (with Cys1254Ala and Thr1273Glu substitutions) and an N-terminal eXact tag

Depositor

Syed Saif Hasan

Article

Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

Insert

Cytosolic tail of SARS-CoV-2 spike carrying an N-terminal eXact tag and Cys1254Ala and Thr1273Glu substitutions (S SARS-CoV-2)

Use

Tags

eXact

Expression

Bacterial

Mutation

Promoter

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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BD-pGEX5X3-BD-SARS-CoV-2-S-tail-Thr1273Asp

Plasmid

#218457

Purpose

Bacterial expression plasmid for the SARS-CoV-2 cytosolic tail (Thr1273Asp substitution) with an N-terminal GST tag

Depositor

Syed Saif Hasan

Article

Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

Insert

Cytosolic tail of SARS-CoV-2 spike carrying Thr1273Asp substitution and an N-terminal GST-tag (S SARS-CoV-2)

Use

Tags

GST

Expression

Bacterial

Mutation

Thr1273Asp substitution

Promoter

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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