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Plasmid#185856PurposeTesting the SkGAL2 promoter inserted with 3 PZ4 elements using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2M2(3*PZ4)>(BamHI)yEGFP>TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10E1A
Plasmid#185855PurposeTesting the SkGAL2 promoter inserted with 2 PZ4 elements using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2M1(2*PZ4)> (BamHI)yEGFP>TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP8EFA
Plasmid#185854PurposeTesting a heat inducible protein degradation tag -DHFR P66L using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1>UBI4>RHGSGTMV>DHFRP66L>yEGFP>TPGK1-TURA3
UseTagsExpressionYeastMutationDHFR P66LPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP3A2 (1)
Plasmid#185851PurposeTesting the TEF1 promoter appended with 2 tetracycline riboswitch using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1>2*TcRb>yEGFP>TPGK1-TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB221
Plasmid#185071PurposeSST::ACT2 "SA236"DepositorInsertACT2
UseTagsExpressionYeastMutationACT2-SA236PromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB215
Plasmid#185068PurposeSST::WAK2 EcoRI/ScaI fragment (secretion confirmed)DepositorInsertWAK2
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB345
Plasmid#185078Purposerfa3D46 truncation fused to GFPDepositorInsertRFA3
UseTagsGFPExpressionYeastMutationRfa3 truncation aa 1-46PromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB394
Plasmid#185086PurposeNMA111 with both nuclear localization signals mutated to alanines fused to GFP (mutagenesis of KBB280)DepositorInsertNMA111
UseTagsGFPExpressionYeastMutationNma111 KKR 9-11 AAA; KRK 28-30 AAAPromoterAvailable sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB349
Plasmid#185080Purposerfa2D248 truncation fused to GFPDepositorInsertRFA2
UseTagsGFPExpressionYeastMutationRfa2 truncation aa 1-247PromoterAvailable sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lifeact-μDpTT
Plasmid#182322PurposeActin-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.DepositorInsertLifeact-μDronpa-TagRFP-T
UseTagsN-terminal 17 amino acids from budding yeast Abp1…ExpressionMammalianMutationDronpa contains H193T mutation.PromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pML107-kanR468
Plasmid#162041PurposeExpresses Cas9 and contains kanR468 guide which targets the kanR geneDepositorInsertkanR468 guide
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pML104-natR412
Plasmid#162042PurposeExpresses Cas9 and contains natR412 guide which targets the natR geneDepositorInsertnatR412 guide
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pML107-natR854
Plasmid#162043PurposeExpresses Cas9 and contains natR854 guide which targets the natR geneDepositorInsertnatR854 guide
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-Rnt1-dsRBD(+6A)
Plasmid#171552PurposeEncoding part of the budding yeast Rnt1 protein from L366 to S453DepositorInsertRnt1 (RNT1 Budding Yeast)
UseTagsExpressionBacterialMutationa six-alanine insertion in the L1 regionPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-Rnt1-dsRBD(+3A)
Plasmid#171551PurposeEncoding part of the budding yeast Rnt1 protein from L366 to S453DepositorInsertRnt1 (RNT1 Budding Yeast)
UseTagsExpressionBacterialMutationa three-alanine insertion in the L1 regionPromoterAvailable sinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-Rnt1-dsRBD(+6)
Plasmid#171550PurposeEncoding part of the budding yeast Rnt1 protein from L366 to S453DepositorInsertRnt1 (RNT1 Budding Yeast)
UseTagsExpressionBacterialMutationa six amino acid_TRLTEG_insertion in the L1 regionPromoterAvailable sinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
THI ONLY pALD4 T-
Plasmid#161497PurposeNon activation and non targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T-_gRNA_MS2DepositorInsertsdCAS9
MS2 (non activation control NAC)
gRNA (Non targeting control NTC)
UseTagsExpressionYeastMutationPromoterpALD4, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPEE42
Plasmid#139421PurposeRecyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the Citrine ORF and a flexible linker at the 5' end.DepositorInsertCitrine
UseTagsExpressionYeastMutationPromoterTEF1Available sinceFeb. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGST-pol1-N2-2A2
Plasmid#160991PurposeExpress a fragment of yeast pol1 proteinDepositorInsertpol1
UseTagsGSTExpressionBacterialMutationF58A/D62APromoterAvailable sinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGST-pol1-N1-2A2
Plasmid#160990PurposeExpress a fragment of yeast pol1 proteinDepositorInsertpol1
UseTagsGSTExpressionBacterialMutationF58A/D62APromoterAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only