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Plasmid#212770PurposeC-terminal PCR-based tagging with CUbo-AID*-yeGFP in budding yeast with HygR selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only
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pUra3-pCUP1-CUbo(K48R/K63R)
Plasmid#212765PurposeN-terminal PCR-based tagging with pCUP1-CUbo(K48R/K63R) in budding yeast with URA3 selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUra3-pCUP1-CUbo(K63R)
Plasmid#212764PurposeN-terminal PCR-based tagging with pCUP1-CUbo(K63R) in budding yeast with URA3 selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUra3-pCUP1-CUbo(K48R)
Plasmid#212763PurposeN-terminal PCR-based tagging with pCUP1-CUbo(K48R) in budding yeast with URA3 selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUra3-pCUP1-CUbo
Plasmid#212762PurposeN-terminal PCR-based tagging with pCUP1-CUbo in budding yeast with URA3 selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUra3-pCUP1-His7-NUbo
Plasmid#212761PurposeN-terminal PCR-based tagging with pCUP1-His7-NUbo in budding yeast with URA3 selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUra3-pCUP1-NUbo
Plasmid#212760PurposeN-terminal PCR-based tagging with pCUP1-NUbo in budding yeast with URA3 selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST-3xFLAG-Ubc7
Plasmid#212801PurposeConstitutive expression of 3xFLAG-tagged budding yeast Ubc7 in mammalian cellsDepositorInsertUbc7
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Ubc13
Plasmid#212806PurposeConstitutive or doxycycline-inducible expression of budding yeast Ubc13 in mammalian cellsDepositorInsertUbc13
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Mms2
Plasmid#212807PurposeConstitutive or doxycycline-inducible expression of budding yeast Mms2 in mammalian cellsDepositorInsertMms2
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDN0617 (pDEST-mEGFP C-term, NatR)
Plasmid#210484PurposepDEST vector to tag gene of interest with mEGFP on the C-terminus to perform localization assays in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…TagsmEGFPExpressionYeastMutationPromoterADH1Available sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRH3243
Plasmid#210187PurposeProtein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::aro7-R33P-GFP::tADH1DepositorInsertYIp ADE2-LEU2 pTDH3::aro7-R33P-GFP::tADH1 (ARO7 Budding Yeast)
UseTagsExpressionYeastMutationR33PPromoterAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRH3176
Plasmid#210178PurposeProtein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::aro7-G141S-GFP::tADH1DepositorInsertYIp ADE2-LEU2 pTDH3::aro7-G141S-GFP::tADH1 (ARO7 Budding Yeast)
UseTagsExpressionYeastMutationG141SPromoterAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRH3145
Plasmid#210169PurposeProtein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::aro7-D147V-GFP::tADH1DepositorInsertYIp ADE2-LEU2 pTDH3::aro7-D147V-GFP::tADH1 (ARO7 Budding Yeast)
UseTagsExpressionYeastMutationD147VPromoterAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRH3139
Plasmid#210167PurposeProtein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::aro7-R33G-GFP::tADH1DepositorInsertYIp ADE2-LEU2 pTDH3::aro7-R33G-GFP::tADH1 (ARO7 Budding Yeast)
UseTagsExpressionYeastMutationR33GPromoterAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRH3142
Plasmid#210168PurposeProtein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::aro7-D147G-GFP::tADH1DepositorInsertYIp ADE2-LEU2 pTDH3::aro7-D147G-GFP::tADH1 (ARO7 Budding Yeast)
UseTagsExpressionYeastMutationD147GPromoterAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRH3175
Plasmid#210177PurposeProtein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::aro7-R33G,G141S-GFP::tADH1DepositorInsertYIp ADE2-LEU2 pTDH3::aro7-R33G,G141S-GFP::tADH1 (ARO7 Budding Yeast)
UseTagsExpressionYeastMutationR33G,G141SPromoterAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPT-TAR C1
Plasmid#141246PurposeTransformation Associated Recombination (TAR) cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 1DepositorInsertMitochondrial Genome of Phaeodactylum tricornutum
UseSynthetic Biology; Diatom expressionTagsExpressionBacterial and YeastMutationMinor additional mutations described in manuscriptPromoterAvailable sinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPT-PCR C1
Plasmid#141248PurposePCR cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 1DepositorInsertReduced Mitochondrial Genome of Phaeodactylum tricornutum
UseSynthetic Biology; Diatom expressionTagsExpressionBacterial and YeastMutationRepeat region removed, additional minor mutations…PromoterAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
UG75-PAH1-3HA
Plasmid#184761PurposePAH1 overexpression. Promotes the formation of diacylglycerol from phosphatidate. Uses antibiotic resistance marker hphMX6.DepositorInsertPAH1 (PAH1 Budding Yeast)
UseTags3HAExpressionYeastMutationPromoterPAH1Available sinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only