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pEGFP-C1-hAtg2A

Plasmid

#36456

Depositor

Noboru Mizushima

Article

Velikkakath et al Mol Biol Cell. 2012 Mar;23(5):896-909. E

Insert

autophagy-related protein 2 homolog A (ATG2A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

changed Proline 656 to Arginine

Promoter

CMV

Available since

May 10, 2012

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_EML4_ALK

Plasmid

#205809

Purpose

Express mEGFP-tagged fusion protein, EML4_ALK from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

EML4_ALK (EML4, ALK Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pT7TS

Plasmid

#17091

Depositor

Paul Krieg

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

June 5, 2008

Availability

Academic Institutions and Nonprofits only

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pBABE-puro-GFP-wt-lamin A

Plasmid

#17662

Depositor

Tom Misteli

Article

Scaffidi et al Nat Cell Biol. 2008 Mar 2. ():.

Insert

GFP-lamin A (LMNA Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pMRX-IPU-GFP-MYH9

Plasmid

#168273

Purpose

Expresses MYH9 in mammalian cells

Depositor

Noboru Mizushima

Article

Yamamoto et al Nat Commun. 2021 Jun 2;12(1):3292. doi:

Insert

MYH9 (MYH9 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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AAV2/9-CW3SL-mCherry-DrTrkB

Plasmid

#184002

Purpose

AAV production for expression of optically controlled eDrTrkB with N-terminal fusion of mCherry

Depositor

Dmitry Gerashchenko, Vladislav Verkhusha

Article

Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

Insert

eDrTrkB (NTRK2 Human)

Use

AAV

Tags

Ig kappa secretory signal, mCherry, Deinococcus r…

Expression

Mutation

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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plenti.CamKII.(synapto).iATPSnFR2.A95A.A119L.miRFP670nano3

Plasmid

#209730

Purpose

Expresses presynaptic low affinity ratiometric ATP sensor

Depositor

Timothy Ryan

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

(synapto).iATPSnFR2.A95A.A119L.miRFP670nano3

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CamKII

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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Lamp1-mCherry-RUSH

Plasmid

#202799

Purpose

RUSH cargo: Str-KDEL_IRES_SBP-mCherry-Lamp1

Depositor

Juan Bonifacino

Article

Chen et al J Cell Biol. 2017 Dec 4;216(12):4141-415

Insert

Lamp1 (Lamp1 Rat)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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psPax2-D64V-NC-mNeon

Plasmid

#196509

Purpose

mNeon fluorescent protein fused to nucleocapsid protein in psPAX2

Depositor

Howard Chang

Article

Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

Insert

mNeon

Use

Tags

mNeon

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pZac2.1-GfaABC1D-TurboID(C )-HA-GPI

Plasmid

#163697

Purpose

Expresses c-terminal half of TurboID on surface of astrocytes

Depositor

Scott Soderling

Article

Takano et al Nature. 2020 Dec;588(7837):296-302. doi:

Insert

GPI anchored TurboID

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-dCas9-3XGFP

Plasmid

#64107

Purpose

Expression of Sp dCas9-3XGFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp dCas9

Use

Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Mutation

Promoter

CMV-TO

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pJSK663

Plasmid

#205129

Purpose

Expression of GFP-1xPX (from SnxA) in mammalian cells

Depositor

Jason King

Article

Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

Insert

1xPX domain of SnxA

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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RED

Plasmid

#139835

Purpose

Expresses RNase H1-EGFP- dCas9 fusion protein in human cells

Depositor

Karim Mekhail

Article

Abraham et al Nature. 2020 Sep;585(7824):298-302. doi:

Insert

RNase H1-EGFP-dCas9 (RNASEH1 RNase H1 (H. sapiens), dCas9 (S. pyogenes))

Use

Tags

Expression

Mammalian

Mutation

dCas9 protein contains two mutations: D10A and H8…

Promoter

tet-inducible CMV

Available since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pBL132

Plasmid

#104333

Purpose

Expresses taz from a LacI regulated promoter under inducible IPTG control.

Depositor

Jeffrey Tabor

Article

Landry et al Nat Commun. 2018 Apr 12;9(1):1433. doi:

Insert

Taz

Use

Tags

Expression

Bacterial

Mutation

Promoter

PtacI

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-jAspSnFR3-mRuby3-NLS

Plasmid

#203494

Purpose

Nuclear directed jAspSnFR3-mRuby3 in lentiviral expression vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

jAspSnFR3-mRuby3-NLS

Use

Lentiviral

Tags

Nuclear localization signal and mRuby3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pLYS1-FLAG-MitoGFP-HA

Plasmid

#50057

Purpose

Expression of GFP targeted to mitochondria with Flag and HA tags

Depositor

Vamsi Mootha

Article

Sancak et al Science. 2013 Nov 14.

Insert

Mito-GFP

Use

Lentiviral

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only

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NES-mCherry-P4Mx1

Plasmid

#108143

Purpose

PI4P biosensor

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

X.leavis map2k1.L(32-44):mCherry:L. penomophila SidM(546-647)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3Luc-5XNF-kappaB

Plasmid

#185695

Purpose

Reporter plasmid with a double-strand oligonucleotides that contain five repeats of NF-kappaB binding consensus sequence (5'-GGGGAATTTCC-3').

Depositor

Esther López-Bayghen

Article

Hernandez-Melchor et al ASN Neuro. 2022 Jan-Dec;14:1759091422111

Insert

NF-kappaB (NFKB1 Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

NF-kappaB binding site

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-Gm-eyfp

Plasmid

#65031

Purpose

GmR mini-Tn7 vector, mobilizable, for YFP tagging bacteria

Depositor

Herbert Schweizer

Article

Choi et al Nat Protoc. 2006;1(1):153-61.

Insert

eyfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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Lenti_sgRNA(MS2)_MCP-VP64-IRES-zsGreen1

Plasmid

#138461

Purpose

lenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-VP64

Depositor

Jian Xu

Article

Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

Insert

MCP-VP64-IRES-zsGreen1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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