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pFS_0351_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_1

Plasmid

#116963

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pET31b-T7-Spinach2-Ct

Plasmid

#79158

Purpose

Expresses the Ct biosensor under a T7 promoter

Depositor

Ming Hammond

Article

Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

Insert

T7-tRNA-Spinach2-Ct

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pET16b-Mms2

Plasmid

#18893

Depositor

Cecile Pickart

Article

Raasi et al Methods Mol Biol. 2005 . 301():47-55.

Insert

Yeast ubiquitin conjugating enzyme Mms2 (MMS2 Budding Yeast)

Use

Tags

HIS10

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 4, 2008

Availability

Academic Institutions and Nonprofits only
pET28-mTDGa.0

Plasmid

#81050

Purpose

bacterial expression of murine TDG

Depositor

Primo Schaer

Article

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

TDG (Tdg Mouse)

Use

Tags

6HIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pET21a-EthR

Plasmid

#51170

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

EthR

Use

Synthetic Biology

Tags

6x-his

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pET28b-pvdA WT

Plasmid

#87098

Purpose

expresses PvdA, the ornithine hydroxylase from Pseudomonas aeruginosa

Depositor

Audrey Lamb

Article

Olucha et al J Biol Chem. 2011 Sep 9;286(36):31789-98

Insert

pvdA WT

Use

Tags

his-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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Ypet-PBD

Plasmid

#105290

Purpose

To measure Rac or Cdc42 activity by binding and FRET analysis

Depositor

Kenneth Yamada

Article

Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

Insert

Pak1 (PAK1 Human)

Use

Tags

Ypet

Expression

Mammalian

Mutation

aa encoding 65-150

Promoter

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pET15b/BOA

Plasmid

#82041

Purpose

For E.coli expression of 6xHis-Tagged BOA (Burkholderia oklahomensis agglutinin)

Depositor

Angela Gronenborn

Article

Whitley et al FEBS J. 2013 May;280(9):2056-67. doi: 10

Insert

Burkholderia oklahomensis agglutinin

Use

Tags

6x His

Expression

Bacterial

Mutation

Promoter

Available since

June 21, 2017

Availability

Academic Institutions and Nonprofits only

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pUBQ10::ZF108_3xFLAG_YPet

Plasmid

#106441

Purpose

pUBQ10 driven Zinc Finger YPet fusion that targets the FWA promoter in arabidopsis

Depositor

Steven Jacobsen

Article

Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

Insert

ZF108_3xFLAG_YPet

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pET21c-IF3mt

Plasmid

#31078

Depositor

Linda Spremulli

Article

Koc et al J Biol Chem. 2002 Sep 20. 277(38):35541-

Insert

Mature form human mitochondrial translational initiation factor 3 (MTIF3 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Includes coding sequence for the mature form. Ami…

Promoter

Available since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
pET22-HT/plsA

Plasmid

#73625

Purpose

Produces Escherichia coli adenylate kinase (PlsA), N-terminal His6 tag

Depositor

James Williamson, T. J. Kappock

Article

Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

Insert

adenylate kinase (adk Escherichia coli str. K-12 substr. MG1655)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pET30b-7D-SrtA

Plasmid

#105600

Purpose

expresses 7D sortase A variant in E.coli

Depositor

Stephanie Dougan

Article

Jeong et al PLoS One. 2017 Dec 4;12(12):e0189068. do

Insert

S.aureus Sortase A, 7D mutant

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pET15b-mISG15(GG)

Plasmid

#12448

Depositor

Dong-Er Zhang

Article

Kim et al Methods Enzymol. 2005 . 398():491-9.

Insert

C-terminal processed form of ISG15

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only
pET15b-Opt13

Plasmid

#61698

Purpose

Expresses PTDH mutant Opt13

Depositor

Huimin Zhao

Article

McLachlan et al Biotechnol Bioeng. 2008 Feb 1;99(2):268-

Insert

Opt13

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 3, 2015

Availability

Academic Institutions and Nonprofits only
pET28a-PAN

Plasmid

#200971

Purpose

Express poly-histidine tagged Methanococcus jannaschii PAN in E. coli recombinantly

Depositor

Yifan Cheng

Article

Yu et al Nat Commun. 2020 Sep 11;11(1):4580. doi:

Insert

PAN

Use

Tags

6xHis and 6xHis-thrombin site

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pET3a-DJ1

Plasmid

#51489

Purpose

Inducible expression of human DJ1 in E.coli

Depositor

Michael J Fox Foundation MJFF

Insert

DJ1 (PARK7 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 31, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pET15-MTFmt

Plasmid

#31174

Depositor

Linda Spremulli

Article

Takeuchi et al J Biol Chem. 1998 Jun 12. 273(24):15085-

Insert

Mature form bovine mitochondrial methionyl-tRNA transformylase (MTFMT Bovine)

Use

Tags

His and thrombin cleavage

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pET28a MDH_PFALCI

Plasmid

#204235

Purpose

Bacterial expression of unicellular protozoan parasite P. falciparum malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

Depositor

Malate dehydrogenase CUREs Community (MCC)

Insert

MDH_PFALCI (PF3D7_0618500 Plasmodium falciparum)

Use

Tags

6X His

Expression

Bacterial

Mutation

Promoter

Lac

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pET21a - SodA

Plasmid

#89612

Purpose

Expresses recombinant 6HIS tagged T. cruzi superoxide dismutase A protein in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Superoxide dismutase A

Use

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Mutation

Promoter

Available since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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YPet-Golgi-7

Plasmid

#56633

Purpose

Localization: Golgi, Excitation: 513, Emission: 527

Depositor

Michael Davidson

Insert

Golgi (B4GALT1 Human)

Use

Tags

YPet

Expression

Mammalian

Mutation

aa 1-82 of NM_001497.3

Promoter

CMV

Available since

Dec. 18, 2014

Availability

Industry, Academic Institutions, and Nonprofits