Skip to main content
Addgene
Showing: 821 - 840 of 3897 results
  1. pJYDN3

    Plasmid
    #162456
    Purpose
    Yeast display platform plasmid N3 which allows for simultaneous expression of the protein of interest at the N-terminus of Aga2p yeast agglutinin and eUnaG2 or nbALFA detection.
    Depositor
    Type
    Empty backbone
    Use
    Shuttle vector bacteria/yeast
    Tags
    ALFA tag, HA, myc tags, and eUnaG2 fluorescent pr…
    Expression
    Mutation
    Promoter
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. ttAtCas12a+int

    Plasmid
    #182384
    Purpose
    Gives very high editing efficiency in barley. Also works in Brassica oleracea
    Depositor
    Insert
    LbCas12a coding sequence with D156R and Arabidopsis introns
    Use
    CRISPR
    Tags
    Expression
    Mutation
    D156R + 8 Arabidopsis introns added
    Promoter
    None
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
  3. pYHY3

    Plasmid
    #216109
    Purpose
    aTC-inducible expression of secretory BT3630 SP-NanoLuc via P1TDP-A21 promoter-RBS pair in Bacteroides species.
    Depositor
    Insert
    NanoLuc
    Use
    Synthetic Biology; E. coli-bacteroides shuttle ve…
    Tags
    3xFLAG tag and BT3630 signal peptide
    Expression
    Bacterial
    Mutation
    Promoter
    P1TDP-A21
    Available since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  4. pJYDN2

    Plasmid
    #162454
    Purpose
    Yeast display plasmid intended for co-cultivation labeling by using designed ALFA-tag binding nanobody (DnbALFA) and expression of N-terminal fusions with Aga2p yeast agglutinin.
    Depositor
    Type
    Empty backbone
    Use
    Shuttle vector bacteria/yeast
    Tags
    DnbALFA nanobody and Ha and myc
    Expression
    Mutation
    Promoter
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
  5. pCD86-mEGFP

    Plasmid
    #190745
    Purpose
    Expression of mEGFP-tagged CD86 in mammalian cells.
    Depositor
    Insert
    CD86 B7-2, B7.2, B70, CD28LG2, LAB (CD86 Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. pJAT23

    Plasmid
    #204312
    Purpose
    For generating targeting inversions. C-terminal split GFP, expressed in eyes.
    Depositor
    Insert
    3XP3-GFPc
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    3XP3
    Available since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
  7. pEF1α-Cre-2A-mScarlet

    Plasmid
    #207745
    Purpose
    Expression of the Cre recombinase linked to a mScarlet fluorescent marker
    Depositor
    Insert
    Cre-PGK-mScarlet
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1alpha
    Available since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
  8. pJMH147

    Plasmid
    #186647
    Purpose
    aadA.co-TaadA-J23107-4ABH.co.rm1-PpsaA-nhoA.co.rm1 between homology arms for pilA
    Depositor
    Insert
    aadA.co-TaadA-J23107-4ABH.co.rm1-PpsaA-nhoA.co.rm1
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
  9. pJYDN2p

    Plasmid
    #162455
    Purpose
    Positive control plasmid for co-cultivation labeling by using designed ALFA-tag binding nanobody (DnbALFA).
    Depositor
    Insert
    Aga2p-DnbALFA (AGA2 )
    Use
    Shuttle vector bacteria/yeast
    Tags
    DnbALFA nanobody and HA and myc
    Expression
    Mutation
    Promoter
    GAL1
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
  10. pKB225

    Plasmid
    #170015
    Purpose
    Encodes LacI-controlled expression of surface display protein with no C-terminal peptide and PvirK-mNeonGreen reporter
    Depositor
    Inserts
    mNeonGreen
    Peptide display protein with no C-terminal peptide
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Mutation
    Promoter
    Ptac and PvirK
    Available since
    Nov. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
  11. pTH849-gst-optec

    Plasmid
    #89590
    Purpose
    Plasmid for bacterial expression of His-tagged GST encoded by preferred codons
    Insert
    glutathione-S transferase codon-optimised for bacterial expression
    Use
    Tags
    8xhistidine
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 821 - 840 of 3897 results