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AktPH-mCherry-T2A-PHR-T2A-CIBNcaax

Plasmid

#173861

Purpose

Mammalian expression of Akt PH-mCherry-T2A-PHR-T2A-CIBNcaax (PIP3 marker and opto-control)

Depositor

Takao Nakata

Article

Kakumoto et al PLoS One. 2013 Aug 7;8(8):e70861. doi: 1

Insert

AktPH-mCherry-T2A-PHR-T2A-CIBNcaax

Use

Tags

C-terminal CAAX-box and mCherry

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pFX-Lifeact-iRFP

Plasmid

#175823

Purpose

Express iRFP with actin-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

iRFP with actin-targeting sequence

Use

Tags

Lifeact (N-terminal 17 amino acids from S. Cerevi…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-Lifeact-Venus

Plasmid

#174478

Purpose

Express Venus with actin-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Venus with actin-targeting sequence

Use

Tags

Lifeact (N-terminal 17 amino acids from S. Cerevi…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-bSV1_1-1009

Plasmid

#129471

Purpose

Mammalian expression of the N-terminus + central domain of bovine SV1

Depositor

Elizabeth Luna

Article

Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

Insert

Supervillin (SVIL Bovine)

Use

Tags

Expression

Mammalian

Mutation

contains amino acids 1-1009

Promoter

CMV

Available since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pSG5-H-Ras_V12

Plasmid

#39510

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 (HRAS Human)

Use

Eukaryotic

Tags

Expression

Mutation

G12V

Promoter

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pBa.Halo-Kif13B tail 442-1843

Plasmid

#162721

Purpose

Mammalian expression of WT mouse Kif13b with deletion of the 441 N-terminal residues

Depositor

Marvin Bentley

Article

Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

Insert

Kif 13B (Kif13b Mouse)

Use

Tags

Expression

Mammalian

Mutation

WT with deletion of the 441 N-terminal residues.

Promoter

Chicken Beta actin

Available since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP TEM4 DEL NTERM

Plasmid

#58894

Purpose

Expresses GFP tagged TEM4 with N terminus deleted

Depositor

Channing Der

Article

Mitin et al PLoS One. 2012;7(7):e41876. doi: 10.1371

Insert

TEM4 (ARHGEF17 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Deleted N Terminus up to aa 1002

Promoter

CMV

Available since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only
pCMV5-Flag-EPLIN beta delta C term

Plasmid

#40931

Depositor

Elizabeth Luna, Scott Maul

Article

Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

Insert

EPLIN beta (LIMA1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

C terminus deleted

Promoter

Available since

Dec. 12, 2012

Availability

Academic Institutions and Nonprofits only
pMX-Flag-WIP 460 deletion

Plasmid

#11773

Depositor

Jack Strominger

Article

Krzewski et al J Cell Biol. 2006 Apr 10. 173(1):121-32.

Insert

WIP aa1-460 (WIPF1 Human)

Use

Retroviral

Tags

flag

Expression

Mammalian

Mutation

aa461-503 deleted

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pBABE-puroGFP-Eps8WT

Plasmid

#74956

Purpose

mammalian expression of Eps8 fused to GFP

Depositor

Giorgio Scita

Article

Frittoli et al Immunity. 2011 Sep 23;35(3):388-99. doi:

Insert

Eps8 (Eps8 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 3, 2016

Availability

Academic Institutions and Nonprofits only
pCMV5-Flag-EPLIN alpha delta N54

Plasmid

#40934

Depositor

Elizabeth Luna, Scott Maul

Article

Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

Insert

EPLIN (LIMA1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

deleted amino acids 1-54

Promoter

Available since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only
pCMV5-Flag-EPLIN alpha delta N112

Plasmid

#40935

Depositor

Elizabeth Luna, Scott Maul

Article

Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

Insert

EPLIN (LIMA1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

deleted amino acids 1-112

Promoter

Available since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only
pCMV5-Flag-EPLIN alpha delta N173

Plasmid

#40936

Depositor

Elizabeth Luna, Scott Maul

Article

Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

Insert

EPLIN (LIMA1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

deleted amino acids 1-173

Promoter

Available since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only
px552-sg-gria1-HT

Plasmid

#187652

Purpose

Contains HaloTag to be inserted into the NTD of Gria1 (via HITI), single guide RNA to target Cas9 to Gria1 under control of the U6 promoter, and miRFP670 under control of human synapsin promoter.

Depositor

Erin O'Shea

Article

Wong et al Elife. 2024 Aug 15;13:e80622. doi: 10.75

Inserts

HaloTag donor sequence
miRFP670

Use

AAV and CRISPR

Tags

N/A

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-KIF13AS1371A

Plasmid

#224409

Purpose

Mammalian expression of KIF 13a S1371A

Depositor

Marvin Bentley

Article

Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

Insert

KIF 13a S1371A (Kif13a Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

S1371A

Promoter

Chicken Beta actin

Available since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-KIF13AWLDLE

Plasmid

#224410

Purpose

Mammalian expression of KIF 13a WLDLE

Depositor

Marvin Bentley

Article

Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

Insert

KIF 13a WLDLE (Kif13a Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

WLDLE

Promoter

Chicken Beta actin

Available since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pBa.M6PR-GFP

Plasmid

#224407

Purpose

Mammalian expression of M6PR

Depositor

Marvin Bentley

Article

Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

Insert

M6PR (M6PR Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Chicken Beta actin

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-VcnTS-E1015A-E1021A

Plasmid

#213417

Purpose

Vinculin tension sensor (VcnTS) with both directionally asymmetric, force strengthening (DAFS) variant point mutations (E1015A and E1021A).

Depositor

Brenton Hoffman

Article

Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

Insert

VcnTS-E1015A-E1021A (VCL Chicken, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Mutated vinculin glutamic acid 1015 and 1021 to a…

Promoter

CMV

Available since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRRL-VcnTS-E1015A-E1021A

Plasmid

#213411

Purpose

Vinculin tension sensor (VcnTS) with both directionally asymmetric, force strengthening (DAFS) variant point mutations (E1015A and E1021A), in lentiviral expression vector.

Depositor

Brenton Hoffman

Article

Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

Insert

VcnTS-E1015A-E1021A (VCL Chicken, Synthetic)

Use

Lentiviral

Tags

Expression

Mutation

Mutated vinculin glutamic acid 1015 and 1021 to a…

Promoter

CMV

Available since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-VcnCS-E1015A-E1021A

Plasmid

#213414

Purpose

Vinculin conformation sensor (VcnCS) with both directionally asymmetric, force strengthening (DAFS) variant point mutations (E1015A and E1021A).

Depositor

Brenton Hoffman

Article

Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

Insert

VcnCS-E1015A-E1021A (VCL Chicken, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Mutated vinculin glutamic acid 1015 and 1021 to a…

Promoter

CMV

Available since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge