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Plasmid#84582PurposeExpresses mCherry-tagged PH-domain of SWAP70 in mammalian cells, binds to PI(3,4)P2DepositorInsertSWAP70 residues 209-306 (SWAP70 Human)
UseTagsmCherryExpressionMammalianMutationPleckstrin-homology domain, residues 209-306PromoterCMVAvailable sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Flag-EPLIN delta N-term
Plasmid#40932DepositorInsertEPLIN (LIMA1 Human)
UseTagsFlagExpressionMammalianMutationdeleted amino acids 1-220PromoterAvailable sinceDec. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GFP-PQRv3-RFP
Plasmid#73951PurposeProtein Quantitation Reporter (PQR) to quantify a protein of interest in mammalian cells.DepositorInsertssfGFP (superfolder GFP)
RFP
UseTagsA residual PQR peptide wii be left at the C-termi…ExpressionMammalianMutationPromoterChicken beta actin and Chicken beta actin (shared…Available sinceJan. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-2_dCrk
Plasmid#131146PurposeExpresses GST::dCrk (based on crk isoform RA).DepositorInsertCrk (Crk Fly)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2+MT-hToca-1-MGD
Plasmid#33033DepositorInsertToca-1 (FNBP1L Human)
UseTags6xMycExpressionMammalianMutationMGD = amino acid substitutions M383I, G384S and D…Promotersimian CMV IE94Available sinceNov. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFX-Lifeact-mCherry
Plasmid#175822PurposeExpress mCherry with actin-targeting sequence in mammalian cellsDepositorInsertmCherry with actin-targeting sequence
UseTagsLifeact (N-terminal 17 amino acids from S. Cerevi…ExpressionMammalianMutationPromoterCMBAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBA-mKeima-PIM
Plasmid#174650PurposeLow level expression of mKeima-PIM. Can be used for ratiometric detection of inducible aggregate acidification after addition of AP20187. mKeima fluorophore allows for simultaneous GFP-tagged imaging.DepositorInsertxXFKBP homo-mKeima-2x FKBP homo-2x FKBP
UseTagsExpressionMammalianMutationVal24Glu, Tyr80Cys, Ala95Thr mutations in second …PromoterChicken beta-actinAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmiR-report-hEZH2
Plasmid#112543Purpose3'UTR reporter assayDepositorInsertEZH2 (EZH2 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJuly 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-POLArISact
Plasmid#164970PurposeT7 promotor drives in vitro transcription of POLArISact with a human kozak sequenceDepositorInsertPOLArISact
UseIn vitro transcriptionTagsExpressionMutationPromoterT7Available sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-Myo 1e
Plasmid#134905PurposeExpression of rat Myo 1e (Myr 3) in mammalian cells.DepositorInsertMyo 1e (rat) with 3' UTR (Myo1e Rat)
UseTagsEGFPExpressionMammalianMutationPromoterCMV IEAvailable sinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSG5-HA-H-Ras_wt
Plasmid#39509DepositorInsertHRAS (HRAS Human)
UseEukaryoticTagsHAExpressionMutationPromoterAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
AktPH-mCherry-T2A-PHR-T2A-CIBNcaax
Plasmid#173861PurposeMammalian expression of Akt PH-mCherry-T2A-PHR-T2A-CIBNcaax (PIP3 marker and opto-control)DepositorInsertAktPH-mCherry-T2A-PHR-T2A-CIBNcaax
UseTagsC-terminal CAAX-box and mCherryExpressionMammalianMutationPromoterCAGAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFX-Lifeact-iRFP
Plasmid#175823PurposeExpress iRFP with actin-targeting sequence in mammalian cellsDepositorInsertiRFP with actin-targeting sequence
UseTagsLifeact (N-terminal 17 amino acids from S. Cerevi…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-Lifeact-Venus
Plasmid#174478PurposeExpress Venus with actin-targeting sequence in mammalian cellsDepositorInsertVenus with actin-targeting sequence
UseTagsLifeact (N-terminal 17 amino acids from S. Cerevi…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-bSV1_1-1009
Plasmid#129471PurposeMammalian expression of the N-terminus + central domain of bovine SV1DepositorInsertSupervillin (SVIL Bovine)
UseTagsExpressionMammalianMutationcontains amino acids 1-1009PromoterCMVAvailable sinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSG5-H-Ras_V12
Plasmid#39510DepositorInsertHRAS V12 (HRAS Human)
UseEukaryoticTagsExpressionMutationG12VPromoterAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBa.Halo-Kif13B tail 442-1843
Plasmid#162721PurposeMammalian expression of WT mouse Kif13b with deletion of the 441 N-terminal residuesDepositorInsertKif 13B (Kif13b Mouse)
UseTagsExpressionMammalianMutationWT with deletion of the 441 N-terminal residues.PromoterChicken Beta actinAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP TEM4 DEL NTERM
Plasmid#58894PurposeExpresses GFP tagged TEM4 with N terminus deletedDepositorInsertTEM4 (ARHGEF17 Human)
UseTagsEGFPExpressionMammalianMutationDeleted N Terminus up to aa 1002PromoterCMVAvailable sinceSept. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Flag-EPLIN beta delta C term
Plasmid#40931DepositorInsertEPLIN beta (LIMA1 Human)
UseTagsFlagExpressionMammalianMutationC terminus deletedPromoterAvailable sinceDec. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMX-Flag-WIP 460 deletion
Plasmid#11773DepositorInsertWIP aa1-460 (WIPF1 Human)
UseRetroviralTagsflagExpressionMammalianMutationaa461-503 deletedPromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only