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Plasmid#62433PurposeMXS_chaining vector with P2ADepositorInsertSelf-cleaving peptide
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
MXS_bGpA
Plasmid#62424PurposeMXS_chaining vector with bGpADepositorInsertbeta-Globin poly A
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJUMP24-1A(sfGFP)
Plasmid#126971PurposeLevel 1 vector. sfGFP reporter. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJAT21
Plasmid#204309PurposeFor generating targeting inversions. N-terminal split GFP, expressed in abdomen and mouthpartsDepositorInsertie1-GFPn
UseCRISPRTagsExpressionMutationPromoterie1Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJAT25
Plasmid#204310PurposeFor generating targeting inversions. C-terminal split GFP, expressed in abdomen and mouthparts.DepositorInsertie1-GFPc
UseCRISPRTagsExpressionMutationPromoterie1Available sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKIKan_idCas9_Chr_center
Plasmid#149637Purposesuicide vector for knock in into the B. burgdorferi chromosomeDepositorInsertdCas9, lacI
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30Available sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEN_TRE_GFP
Plasmid#25767PurposeEntry vector with TRE promoter driving GFP expression.DepositorTypeEmpty backboneUseEntry vectorTagsGFPExpressionMutationPromoterAvailable sinceJuly 14, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
Bxb1-surrogate-reporter
Plasmid#65624PurposeFluorescent reporter to monitor the activty of Bxb1DepositorInsertattP
UseBxb1TagsExpressionMutationPromoterAvailable sinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDB4282
Plasmid#98701PurposeA plasmid containing the 3' portion of the ura4 marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-ura4 systemDepositorInsertgRNA PCR template
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDB4283
Plasmid#98702PurposeA plasmid containing the 3' portion of the bsdMX marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-bsdMX systemDepositorInsertgRNA PCR template
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEN_TTmiRc2
Plasmid#25752PurposeEntry vector for cloning miR30-based shRNA driven by TRE-tight promoter.DepositorTypeEmpty backboneUseEntry vectorTagsExpressionMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
mSA-phi29
Plasmid#210815Purposein vitro transcription of mRNADepositorInsertmSA-Phi29
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJAT27
Plasmid#204308PurposeFor generating targeting inversions. C-terminal split GFP, expressed in flight muscles.DepositorInsertMHC-GFPc
UseCRISPRTagsExpressionMutationPromoterMHCAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJAT18
Plasmid#204307PurposeFor generating targeting inversions. N-terminal split GFP, expressed in flight muscles.DepositorInsertMHC-GFPn
UseCRISPRTagsExpressionMutationPromoterMHCAvailable sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
MXS_E2-Crimson
Plasmid#62410PurposeMXS_chaining vector with E2-CrimsonDepositorInsertE2-Crimson
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-07
Plasmid#124786PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with mNeonGreenDepositorInsertmNeonGreen
UsePcr templateTagsmNeonGreenExpressionMutationPromoterNoneAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
TOPO mTagBFP2
Plasmid#68413PurposeTOPO construct expressing mTagBFP2 for generation of GMAP-compatible geneDepositorInsertmTagBFP2
UseGmapTags6xHisExpressionBacterialMutationPromoterPlacAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHO-UAS-mScarlet-I
Plasmid#183147PurposeYeat two-hybrid fluorecent reporter for genomic integrationDepositorInsertmScarlet-I
UseTagsExpressionMutationPromoterAvailable sinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJUMP18-merR_O
Plasmid#127023PurposeBasic Part O- ORF; merR; transcription factorDepositorInsertPart merR_o
UseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJYDNgp
Plasmid#162453PurposePositive control plasmid for pJYDNg carrying the eUnaG2 bilirubin dependent fluorescent protein fused to the C-terminus of Aga2p protein. This plasmid contains a long 2G linker.DepositorInsertAga2p-2Glinker-eUnaG2 (AGA2 )
UseShuttle vector bacteria/yeastTagsHA nad myc and eUnaG2 fluorescent proteinExpressionMutationPromoterGAL1Available sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only