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Plasmid#173088PurposeExpression of recombinant fusion protein in E. coliDepositorInsertMACROH2A1 (MACROH2A1 Human)
UseTagsmCeruleanExpressionBacterialMutationQ162-N372, codon optimizedPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
FEMP
Plasmid#191973PurposeFRET-based indicator of ER-mitochondria proximity (FEMP)DepositorInsertAkapYFP-Tav2a-CFP-sac1
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
Nrxn3α-AS4-
Plasmid#184332PurposeExpression of mouse Nrx3-alpha lacking insertion at the alternative splice site 4 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,2,3 and 6.DepositorInsertNrxn3-alpha (Nrxn3 Mouse)
UseTagsHA-tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
MacroH2A1.1-c002
Plasmid#173087PurposeExpression of recombinant fusion protein in E. coliDepositorInsertMACROH2A1 (MACROH2A1 Human)
UseTagsmCeruleanExpressionBacterialMutationQ162-N369, codon optimizedPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nrxn3α-AS4+
Plasmid#184333PurposeExpression of mouse Nrx3-alpha containing insertion at the alternative splice site 4 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,2,3 and 6.DepositorInsertNrxn3-alpha (Nrxn3 Mouse)
UseTagsHA-tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
CoV-2-NSP3-c001
Plasmid#173083PurposeExpression of recombinant fusion protein in E. coliDepositorInsertrep (ORF1ab Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2))
UseTagsmCeruleanExpressionBacterialMutationE1084-E1192, codon optimizedPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST_5xUAS:EB3-mScarlet-I
Plasmid#192358PurposeEB3 tagged with mScarlet-I, expressed under the UAS promoterDepositorInsertEB3 (MAPRE3 Human)
UseTagsmScarlet-IExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR3-deGFP-6xHis-T500
Plasmid#92224PurposeExpression of deGFP using the p70a promoter and UTR3DepositorInsertdeGFP
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationPromoterp70aAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV4-Flag-mIL-17RA/YFP.Hygro
Plasmid#46862Purposeencodes Flag-tagged mouse IL-17RA fused to Yellow Fl. ProteinDepositorInsertIL-17RA-YFP
UseTagsFlag/IL-3 signal sequence and fused to CFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
PXCGW
Plasmid#48255PurposeSplit-GFP assay in plantsDepositorTypeEmpty backboneUseBinary gateway vectorTags6xHIS and CFP aa155-238ExpressionMutationPromoter35SAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
PCXGW
Plasmid#48257PurposeSplit-GFP assay in plantsDepositorTypeEmpty backboneUseBinary gateway vectorTags6xHIS and CFP aa155-238ExpressionMutationPromoter35SAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
BPK1179
Plasmid#179296PurposeExpresses dCas9 fused to four DmrA domainsDepositorInsertdCas9-DmrA(x4)
UseTagsExpressionMammalianMutationD10A, H840A (catalytically inactive Cas9)PromoterCAGAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
ROSA26(MultiFPsΔPuro)
Plasmid#140759PurposeTargeting vector to Gt(ROSA)26 locus for conditional expression of distinct FPs (Venus, mCherry, and mCerulean) responding to each site-specific-recombinase activity (Cre, Dre, and phiC31o).DepositorInsertspuromycin-N-acetyltransferase
Venus
mCherry
mCerulean
UseMouse TargetingTagsExpressionMutationPromoterCAGGSAvailable sinceJune 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-NTerm 6xHis-zmADH-T500
Plasmid#92228PurposeExpression of zmADH using the p70a promoter and UTR1DepositorInsertNTerm 6xHis Tagged zmADH
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationPromoterp70aAvailable sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR3-Nterm 6xHis mRFP-T500
Plasmid#92317PurposeExpression of NTerm 6xHis tagged mRFP using the p70a promoter and UTR3DepositorInsertOptimized NTerm 6xHis tagged monomeric RFP (mRFP)
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationPromoterp70aAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
MERS-coV-NSP3-c001
Plasmid#173085PurposeExpression of recombinant fusion protein in E. coliDepositorInsertrep (orf1ab Middle East respiratory syndrome-related coronavirus (isolate United Kingdom/H123990006/2012) (MERS-CoV))
UseTagsmCeruleanExpressionBacterialMutationD1109-D1275, codon optimizedPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-Nterm 6xHis PL-T500
Plasmid#92314PurposeExpression of NTerm 6xHis tagged protein L (PL) domain using the p70a promoter and UTR1DepositorInsertB1 domain of protein L (PL)
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationPromoterp70aAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70b-UTR1-deGFP-6xHis-T500
Plasmid#92222PurposeExpression of deGFP using the p70b promoter (weak version of the p70a promoter)DepositorInsertdeGFP
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationPromoterp70b (p70a promoter with one mutation)Available sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-Nterm 6xHis mRFP-T500
Plasmid#92316PurposeExpression of NTerm 6xHis tagged mRFP using the p70a promoter and UTR1DepositorInsertOptimized NTerm 6xHis tagged monomeric RFP (mRFP)
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationPromoterp70aAvailable sinceAug. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
p40651-EFSNS-FMR1_e1-24xms2
Plasmid#222968PurposeMS2 mRNA reporter with intact FMR1 5' UTR and exon 1 sequenceDepositorInsertFMR1-31CGG 5' UTR and exon1
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only