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Plasmid#1186DepositorInserthtt 103Q (HTT Human)
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only
These results may also be relevant.
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pLenti-CMV-SMCHD1-R1868*-3xFLAG
Plasmid#130679PurposeExpresses human SMCHD1 R1868* with a C-terminal 3xFLAG tagDepositorInsertSMCHD1 R1868* (SMCHD1 Human)
UseTags3xFLAGExpressionMammalianMutationR1868_V2005delinsXPromoterCMVAvailable sinceJune 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBLIC-neo
Plasmid#45198Purposea ligation-independent cloning (LIC) retroviral vector for stable gene transduction in mammalian cells. Neo selection.DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET19b_4CL:P5SHb + STS:P6SHb
Plasmid#139792Purposeexpression of biosynthetic enzymes 4CL and STS in fusion with CCDepositorInserts4CL:P5SHb
STS:P6SHb
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET19b_4CL:P3S + STS:AP4S
Plasmid#139795Purposeexpression of biosynthetic enzymes 4CL and STS in fusion with CCDepositorInserts4CL:P3S
STS:AP4S
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEL089
Plasmid#137897PurposeiSTU-CRISPR/Cas12a HH-HDV structure crRNA insertion vector with OsCDPK2 intronDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-SMCHD1-Exon37-48-3xFLAG
Plasmid#130688PurposeExpresses human SMCHD1 Exon37-48 with a C-terminal 3xFLAG tagDepositorInsertSMCHD1 Exon37-48 (SMCHD1 Human)
UseTags3xFLAGExpressionMammalianMutation1M_T1522delinsMPromoterCMVAvailable sinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-SMCHD1-Exon1-36-3xFLAG
Plasmid#130681PurposeExpresses human SMCHD1 Exon1-36 with a C-terminal 3xFLAG tagDepositorInsertSMCHD1 Exon1-36 (SMCHD1 Human)
UseTags3xFLAGExpressionMammalianMutationD1523_V2005delinsXPromoterCMVAvailable sinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH99
Plasmid#128204Purposeclassic sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)DepositorInsertclassic sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH84
Plasmid#128195PurposeLevel1 ZmUBIp::A3A-PBE (wheat codon optimized):NOStDepositorInsertZmUBIp::A3A-PBE (wheat codon optimized):NOSt
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH100
Plasmid#128205Purposeimproved sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)DepositorInsertimproved sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTC151
Plasmid#70015PurposeTALENs targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a non-replicating, mutant version of the Gemini Viral RepliconDepositorInsertNuclease (TALENs) + Donor + GVR
UseTALENTagsExpressionPlantMutationPromoterAvailable sinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFH101
Plasmid#128206PurposesgRNA backbone for StCas9, combine with AtU6-26 promoter module (pFH35)DepositorInsertsgRNA backbone for StCas9, combine with AtU6-26 promoter module (pFH35)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH82
Plasmid#128193PurposeLevel1 ZmUBIp::enCas9-Poll3M-TBD (wheat codon optimized):NOStDepositorInsertZmUBIp::enCas9-Poll3M-TBD (wheat codon optimized):NOSt
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH93
Plasmid#128196PurposeLevel1 ZmUBIp::nScCas9-ABE7.10 (wheat/human codon optimized):NOStDepositorInsertZmUBIp::nScCas9-ABE7.10 (wheat/human codon optimized):NOSt
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
PABE-3
Plasmid#115624PurposeTargeted A to G in wheat plants or monocotyledons protoplastsDepositorInsertwtTadA-TadA7.10-NLS-nCas9-NLS
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
PABE-6
Plasmid#115627PurposeTargeted A to G in wheat plants or monocotyledons protoplastsDepositorInsertwtTadA-TadA7.10-nCas9-2*NLS
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
PABE-5
Plasmid#115626PurposeTargeted A to G in wheat plants or monocotyledons protoplastsDepositorInsertNLS-nCas9-NLS-wtTadA-TadA7.10-NLS
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
PABE-4
Plasmid#115625PurposeTargeted A to G in wheat plants or monocotyledons protoplastsDepositorInsertNLS-nCas9-wtTadA-TadA7.10-NLS
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBLIC-puro
Plasmid#45197Purposea ligation-independent cloning (LIC) retroviral vector for stable gene transduction in mammalian cells. Puro selection.DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMiR-HPV16-E1(partial)
Plasmid#53699PurposeLuciferase reporter assay for microRNA binding on HPV16 E1DepositorInsertHPV16-E1(partial)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only