1. p416 103Q GAL

   Plasmid

   #1186

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. pLenti-CMV-SMCHD1-R1868*-3xFLAG

   Plasmid

   #130679

   Purpose

   Expresses human SMCHD1 R1868* with a C-terminal 3xFLAG tag

   Depositor

   Stephen Tapscott

   Article

   Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

   Insert

   SMCHD1 R1868* (SMCHD1 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   R1868_V2005delinsX

   Promoter

   CMV

   Available since

   June 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBLIC-neo

   Plasmid

   #45198

   Purpose

   a ligation-independent cloning (LIC) retroviral vector for stable gene transduction in mammalian cells. Neo selection.

   Depositor

   Priyamvada Rai

   Article

   Patel et al BMC Biotechnol. 2012 Jan 16;12:3. doi: 1

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 19, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pET19b_4CL:P5SHb + STS:P6SHb

   Plasmid

   #139792

   Purpose

   expression of biosynthetic enzymes 4CL and STS in fusion with CC

   Depositor

   Roman Jerala

   Article

   Fink et al AMB Express. 2020 May 24;10(1):97. doi:

   Inserts

   4CL:P5SHb
   STS:P6SHb
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET19b_4CL:P3S + STS:AP4S

   Plasmid

   #139795

   Purpose

   expression of biosynthetic enzymes 4CL and STS in fusion with CC

   Depositor

   Roman Jerala

   Article

   Fink et al AMB Express. 2020 May 24;10(1):97. doi:

   Inserts

   4CL:P3S
   STS:AP4S
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGEL089

   Plasmid

   #137897

   Purpose

   iSTU-CRISPR/Cas12a HH-HDV structure crRNA insertion vector with OsCDPK2 intron

   Depositor

   Yong Zhang

   Article

   Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Feb. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLenti-CMV-SMCHD1-Exon37-48-3xFLAG

   Plasmid

   #130688

   Purpose

   Expresses human SMCHD1 Exon37-48 with a C-terminal 3xFLAG tag

   Depositor

   Stephen Tapscott

   Article

   Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

   Insert

   SMCHD1 Exon37-48 (SMCHD1 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   1M_T1522delinsM

   Promoter

   CMV

   Available since

   Sept. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLenti-CMV-SMCHD1-Exon1-36-3xFLAG

   Plasmid

   #130681

   Purpose

   Expresses human SMCHD1 Exon1-36 with a C-terminal 3xFLAG tag

   Depositor

   Stephen Tapscott

   Article

   Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

   Insert

   SMCHD1 Exon1-36 (SMCHD1 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D1523_V2005delinsX

   Promoter

   CMV

   Available since

   Sept. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFH99

   Plasmid

   #128204

   Purpose

   classic sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   classic sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFH84

   Plasmid

   #128195

   Purpose

   Level1 ZmUBIp::A3A-PBE (wheat codon optimized):NOSt

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   ZmUBIp::A3A-PBE (wheat codon optimized):NOSt
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFH100

    Plasmid

    #128205

    Purpose

    improved sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    improved sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTC151

    Plasmid

    #70015

    Purpose

    TALENs targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a non-replicating, mutant version of the Gemini Viral Replicon

    Depositor

    Daniel Voytas

    Article

    Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

    Insert

    Nuclease (TALENs) + Donor + GVR
    

    Use

    TALEN

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFH82

    Plasmid

    #128193

    Purpose

    Level1 ZmUBIp::enCas9-Poll3M-TBD (wheat codon optimized):NOSt

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    ZmUBIp::enCas9-Poll3M-TBD (wheat codon optimized):NOSt
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFH93

    Plasmid

    #128196

    Purpose

    Level1 ZmUBIp::nScCas9-ABE7.10 (wheat/human codon optimized):NOSt

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    ZmUBIp::nScCas9-ABE7.10 (wheat/human codon optimized):NOSt
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFH101

    Plasmid

    #128206

    Purpose

    sgRNA backbone for StCas9, combine with AtU6-26 promoter module (pFH35)

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    sgRNA backbone for StCas9, combine with AtU6-26 promoter module (pFH35)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PABE-3

    Plasmid

    #115624

    Purpose

    Targeted A to G in wheat plants or monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Li et al Genome Biol. 2018 May 29;19(1):59. doi:

    Insert

    wtTadA-TadA7.10-NLS-nCas9-NLS
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PABE-6

    Plasmid

    #115627

    Purpose

    Targeted A to G in wheat plants or monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Li et al Genome Biol. 2018 May 29;19(1):59. doi:

    Insert

    wtTadA-TadA7.10-nCas9-2*NLS
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PABE-5

    Plasmid

    #115626

    Purpose

    Targeted A to G in wheat plants or monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Li et al Genome Biol. 2018 May 29;19(1):59. doi:

    Insert

    NLS-nCas9-NLS-wtTadA-TadA7.10-NLS
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PABE-4

    Plasmid

    #115625

    Purpose

    Targeted A to G in wheat plants or monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Li et al Genome Biol. 2018 May 29;19(1):59. doi:

    Insert

    NLS-nCas9-wtTadA-TadA7.10-NLS
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBLIC-puro

    Plasmid

    #45197

    Purpose

    a ligation-independent cloning (LIC) retroviral vector for stable gene transduction in mammalian cells. Puro selection.

    Depositor

    Priyamvada Rai

    Article

    Patel et al BMC Biotechnol. 2012 Jan 16;12:3. doi: 1

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 19, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pMiR-HPV16-E1(partial)

    Plasmid

    #53699

    Purpose

    Luciferase reporter assay for microRNA binding on HPV16 E1

    Depositor

    Edward Chan

    Article

    Jung et al Mol Cancer. 2014 Apr 8;13(1):80. doi: 10

    Insert

    HPV16-E1(partial)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only