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Plasmid#219393PurposeExpress CEBPA in mammalian cellsDepositorInsertCEBPA (CEBPA Human)
UseTagsN terminal Myc tagExpressionMammalianMutationPromoterAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
HotPETase_pET21b
Plasmid#215407PurposeExpression construct for HotPETase gene, codon optimized for expression in E. coliDepositorInsertHotPETase
UseTagsExpressionBacterialMutationS121E/D186H/R280A/P181V/S207R/S214Y/Q119K/S213E/N…PromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab7A
Plasmid#28047DepositorInsertRab7A (RAB7A Human)
UseTags2xHA and EGFPExpressionMammalianMutationPromoterAvailable sinceMay 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
Sniper2L
Plasmid#193856PurposeExpresses human codon-optimized Sniper2L and blasticidin resistance: EFS promoter-Sniper2L-NLS-FLAG-P2A-BSDDepositorInsertSniper2L
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationE1007LPromoterEFSAvailable sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
paavCAG-post-mGRASP-2A-dTomato
Plasmid#34912PurposepostDepositorInsertpostsynaptic mGRASP
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceNov. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Luc
Plasmid#45968DepositorInsertLuciferase
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9n(BB)-2A-GFP (PX461)
Plasmid#48140PurposeCas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNADepositorInserthSpCas9n
UseCRISPRTags3XFLAG and GFPExpressionMammalianMutationCas9 D10A nickase mutantPromoterCbhAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
LAMP1-RpHLuorin2
Plasmid#171720PurposeLysosomal expression of ratiometric pHLuorin2DepositorInsertRatiometric pHLuorin2 fused to LAMP1 (LAMP1 Human)
UseTagsratiometric pHluorin2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV G-CEPIA1er
Plasmid#58215PurposeGreen fluorescent indicator for calcium imaging in the endoplasmic reticulumDepositorInsertG-CEPIA1er
UseTagsmycExpressionMammalianMutationG-GECO1.1 E299D M304L D328N N345K F360W G369S E37…PromoterCMVAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFRT_TO_eGFP_TIA1
Plasmid#106094Purposegene expression of EGFP-TIA1DepositorInsertTIA1 (TIA1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-EGFP-KASH
Plasmid#154373PurposeVector for Cre-dependent expression of EGFP tagged KASH protein domainDepositorInsertEGFP-KASH
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-R-iLACCO1.2
Plasmid#208027PurposeBiosensor for intracellular L-lactateDepositorInsertR-iLACCO1.2
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLX_EFS-Cre_ppt-del
Plasmid#162073PurposeCompact Cre expression vector with modified PPT designed to generate high-titer non-integrating lentivirus when packaged with PsPax2-D64V (Addgene #63586)DepositorInsertCre
UseCre/Lox and LentiviralTagsExpressionMammalianMutationcodon-optimized for expression in M. musculusPromoterEFS (part of insert)Available sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9-2A-Puro-TBK1-gRNA (PX459)
Plasmid#221550PurposeExpresses Cas9 and gRNA for disruption of TBK1 gene in human cellsDepositorInsertgRNA targeting human TBK1 (TBK1 Human)
UseTagsExpressionMammalianMutationPromoteru6Available sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-GCaMP6s
Plasmid#40753PurposeMammalian expression of ultrasensitive protein calcium sensorDepositorInsertGCaMP6s
UseTags6xHis, T7 epitope, and Xpress tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEcgRNA
Plasmid#166581PurposeCRISPR-Cas9-assisted genome editing in Escherichia coliDepositorInsertccdB
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cre Reporter
Plasmid#62732PurposeMammalian Cre recombinase reporterDepositorInsertLoxP-DsRED-STOP-LoxP-eGFP
UseCre/Lox and LentiviralTagsLoxP sitesExpressionMammalianMutationPromoterEF1-AlphaAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pG3H-PE3max-attR1R2
Plasmid#213049PurposeDestination vector Expressing nCas9 and RT fusion protein and carrying Gateway recombination sitesDepositorInsertsCas9 nickase
M-MLV Reverse Transcriptase
UseNote: this plasmid needs helper pvs1-vir2 for rep…TagsExpressionPlantMutationH840A, R240K, N413K,PromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCAR_sgRNA_puro-mKate-lox2272
Plasmid#162076Purpose3rd generation lentivirus sgRNA delivery backbone with Cre-removable mKate2 reporter and puromycin resistanceDepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and LentiviralTagsExpressionMammalianMutationPromoterU6, EF1aAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET3a-hUBA1
Plasmid#63571PurposeExpression of human UBA1 tagged with HisDepositorInsertUBA1 (UBA1 Human)
UseTagsExpressionBacterialMutationIsoform 2 (1018 amino acids)PromoterT7Available sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2+MLS-HyPer7
Plasmid#136470PurposeMammalian expression of mitochondrial matrix targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imagingDepositorInsertHyPer7
UseTagsTandem mitochondrial targeting signal of cytochro…ExpressionMammalianMutationPromoterCMV, SP6Available sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
RpHLuorin2
Plasmid#180227Purposefor bacterial expression of recombinant ratiometric pHluorin2DepositorInsertRatiometric pHLuorin2
UseTagsHis-tag followed by thrombin cleavage siteExpressionBacterialMutationPromoterT7Available sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-CMV-GFP-FLAG-cGAS E225A-D227A
Plasmid#86674PurposeExpresses catalytically-inactive GFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.DepositorInsertGFP-FLAG-cGAS E225A/D227A
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) eGFP
Plasmid#129020PurposeExpresses eGFPDepositorInserteGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMA122 - peel-1 negative selection
Plasmid#34873PurposeNegative selection marker for use with the Mos-1 mediated system of creating targeted transgene insertions and targeted deletions in C. elegansDepositorInsertpeel-1 (peel-1 Nematode)
UseWorm targetingTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCas9
Plasmid#167547PurposeUsed for CRISPR-Cas9 mediated recomineering in Enterococcus. Can clone desired gRNA using BsaI digestion.DepositorInsertschloramphenicol acetyl transferase
pUC19 origin of replication
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2-P2A-GFP
Plasmid#132776PurposePrime editing in mammalian cells with co-translational GFP expressionDepositorInsertPE2-P2A-GFP
UseTagsSV40 NLSExpressionMammalianMutationSee manuscriptPromoterCMVAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIR-CMV-SCN1A-Variant-1-IRES-mScarlet
Plasmid#162278PurposeEukaryotic expression of human SCN1A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.DepositorInsertSCN1A Variant 1, stabilized (SCN1A Human)
UseTagsIRES-mScarletExpressionMammalianMutationIVS intron inserted after bp2946. Silent codon c…PromoterCMVAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
rAAV2-retro helper
Plasmid#81070Purposeretrograde AAV helper vector, carrying rep and modified capsid geneDepositorInsertsrep
rAAV2-retro cap
UseAAV and Unspecified; Helper vectorTagsExpressionMutationPro2Ala, possibly Asp613Gly and changes made to A…PromoterAvailable sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB-TRE-EGFP-EF1a-rtTA
Plasmid#104454PurposePiggyBac plasmid expressing EGFP under the control of a doxycycline-inducible promotorDepositorInsertsenhanced green fluorescent protein
reverse tetracycline-controlled transactivator 3
UsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HA-mSTING
Plasmid#214157PurposeTransient expression of STINGDepositorInsertSTING (Sting1 Mouse)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMarch 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
mStayGold-aTubulin_pTwistCMVBetaGlobWPRENeo
Plasmid#221899PurposeExpresses alpha tubulin fused to mStayGoldDepositorInsertmStayGold-aTubulin
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF3084
Plasmid#144560PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZFP36L1 (ZFP36L1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Tau(WT)
Plasmid#187023PurposeExpresses EGFP tagged human 2N4R tau (WT) in mammalian cells with CMV promoterDepositorInsertMAPT (MAPT Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-NE2m
Plasmid#208686PurposeExpresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m in neuronsDepositorInsertGPCR activation based norepinephrine (NE) sensor GRAB_NE2m
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
IRES-puro-GFP
Plasmid#16616DepositorInsertGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
TFORF3476
Plasmid#144952PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSNAI1 (SNAI1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.cTNT.iCre
Plasmid#69916PurposeUsed to package AAV9:cTNT.iCre, which specifically transduce cardiomyocytes and express Cre recombinase. Only works in vivo.DepositorInsertimproved cre (iCre) recombinase and tdTomato
UseAAVTagsThe iCre coding frame and tdTomato coding frame i…ExpressionMutationPromoterChicken cardiac troponin TAvailable sinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
T7-VEE-IRES-Puro
Plasmid#58970Purposenoninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA repliconDepositorTypeEmpty backboneUseVenezuelan equine encephalitis (vee) virus rna re…TagsExpressionMammalianMutationPromoter26S subgenomic promoterAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET21a-Streptavidin-Dead
Plasmid#20859PurposeDead subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Use in combination with Streptavidin-Alive (construct 20860).DepositorInsertStreptavidin-Dead
UseTagsExpressionBacterialMutationTriple mutant of wt Streptavidin N23A,S27D,S45APromoterAvailable sinceApril 21, 2009AvailabilityAcademic Institutions and Nonprofits only