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Plasmid#100931PurposeMammalian expression plasmid for Env from the Q842.d12 HIV-1 strainDepositorInsertHIV-1 (Q842.d12) Env
UseTagsCD5 leader sequenceExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailable sinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6A-EGFR ECD (1-644)
Plasmid#42666DepositorInsertEGFR ECD (1-644) (EGFR Human)
UseTags6xHis and MycExpressionMammalianMutationPromoterAvailable sinceJune 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CHRNA7-mCherry
Plasmid#62632Purposemammalian expression of human Alpha7 (CHRNA7) with mCherry fused into M3-M4 loopDepositorInserthAlpha7 (CHRNA7 Human)
UseTagsExpressionMammalianMutationmCherry inserted into M3-M4 loopPromoterCMVAvailable sinceMarch 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGWB445
Plasmid#74831PurposeGateway cloning compatible binary vector for N-terminal fusion with eCFP (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
22l-COX8-3xFlag-TALE(RNR2-Right)-TadA(8e+V106W)
Plasmid#209296PurposemitoBE expression vector, target mtRNR2 gene.DepositorInsertCOX8-3xFlag-TALE(RNR2-Right)-TadA(8e+V106W)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMVMagFRET-6
Plasmid#50756PurposeExpresses MagFRET-6 in the cytosol of mammalian cells. Weak affinity control FRET sensor for MagFRET-1.DepositorInsertgene encoding for MagFRET-6
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
p426MET25_sfpHluorin (MRV55)
Plasmid#115697Purposesuperfolder pHluorin for intracellular pH measurements in S. cerevisiae (improved performance when targeted to the ER or subsequent compartments)DepositorInsertsfpHluorin
UseTagsExpressionYeastMutationfor introduced mutations see publicationPromoterMET25Available sinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLY092b-lenti-EFS-BCMA-BBz-T2APRODH2-T2APuro-WPRE
Plasmid#192193PurposeLenti-BCMA-CAR-PRODH2DepositorInsertLenti-BCMA-CAR-PRODH2 (TNFRSF17 Synthetic)
UseLentiviral; Mammalian expressionTagsExpressionMutationNAPromoterAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNES-VL12.3
Plasmid#43862DepositorInsertVL12.3
UseTagsHisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBOB-VE-cadherin-tdTomato
Plasmid#118733PurposeTo express VE-cadherin tagged with tdTomato on C-terminusDepositorInsertCadherin 5 (CD144) (CDH5 Human)
UseLentiviralTagstdTomatoExpressionMutationI503T (Common SNP-see comments)PromoterCMVAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
hE-cadherin/Δβ-catenin-pcDNA3
Plasmid#45772DepositorInserthE-cadherin/Δβ-catenin (CDH1 Human)
UseTagsExpressionMammalianMutationEnds at amino acid 844. Last 35 amino acids dele…PromoterCMVAvailable sinceJune 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSHS207 - Bacterial expression plasmid for WT SpCas9
Plasmid#101199PurposeBacterial expression plasmid for WT SpCas9DepositorInsertSpCas9
UseTags10x His, MBP, and TEV siteExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
WT Smarca4-sfGFP
Plasmid#107056PurposeExpression of wild-type Smarca4 (Brg1) with C-terminal super-folder GFP fusion.DepositorInsertSWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
UseLentiviralTagsSuper-folder GFP (sfGFP)ExpressionMammalianMutationwild-typePromoterCAGAvailable sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-R-iLACCO1.2
Plasmid#208101PurposeBiosensor for intracellular L-lactateDepositorInsertR-diLACCO1
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYLsgRNA-AtU3d/LacZ
Plasmid#66201Purposecloning of sgRNAs for expression in plantsDepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterAtU3dAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-HA_WT POLK
Plasmid#201584PurposeExpresses human DNA polymerase kappa with an N-terminal HA tag in mammalian cellsDepositorInsertPolymerase kappa (POLK Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1-EPSIN 1
Plasmid#22228DepositorInsertEpsin 1 (Epn1 Rat)
UseTagsGFPExpressionBacterial and MammalianMutationplease see depositor comment belowPromoterAvailable sinceOct. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSF3-microID
Plasmid#172880Purposeexpresses microID in mammalian cells with the tet-on or tet-off systemDepositorInsertmicroID
UseLuciferase and Retroviral; To be used in a tet-on…TagsmycExpressionMammalianMutationfragment ([aa2-171]) of aquifex aeolicus BirA wit…PromoterTRE-cmvAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
2xNLS-pMJ915v2
Plasmid#88916PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertsCas9
T7
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-R-diLACCO1
Plasmid#208102PurposeControl biosensor for intracellular L-lactateDepositorInserteLACCO2.1
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only