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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 42666 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA6/myc-His A
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5100
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Vector typeMammalian Expression
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameEGFR ECD (1-644)
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Alt nameepidermal growth factor receptor ECD (1-644)
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SpeciesH. sapiens (human)
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Insert Size (bp)600
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Entrez GeneEGFR (a.k.a. ERBB, ERBB1, ERRP, HER1, NISBD2, NNCIS, PIG61, mENA)
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Tags
/ Fusion Proteins
- Myc (C terminal on backbone)
- 6xHis (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (unknown if destroyed)
- 3′ cloning site XbaI (unknown if destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA6A-EGFR ECD (1-644) was a gift from Mien-Chie Hung (Addgene plasmid # 42666 ; http://n2t.net/addgene:42666 ; RRID:Addgene_42666) -
For your References section:
Characterization of a novel tripartite nuclear localization sequence in the EGFR family. Hsu SC, Hung MC. J Biol Chem. 2007 Apr 6;282(14):10432-40. Epub 2007 Feb 5. 10.1074/jbc.M610014200 PubMed 17283074