Addgene

We narrowed to 9,019 results for: yeast

Showing: 8361 - 8380 of 9019 results

Results per page:

Sort results by:

pIRTetR-GAL80

Plasmid

#185853

Purpose

Plugging the mechanism of tetracycline-mediated repression of GAL promoters in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

gal80Arm1-PAgTEF1-KanMX4-TAgTEF1-PHAC1>TetR>TUP1>PABF1- PTEF1+[TetO]>GAL80Arm2

Use

Tags

Expression

Yeast

Mutation

None

Promoter

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pILGFP3A2 (2)

Plasmid

#185852

Purpose

Testing the TEF1 promoter appended with 3 tetracycline riboswitch using a green fluorescence protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1-PTEF1>3*TcRb>yEGFP>TPGK1-TURA3

Use

Tags

Expression

Yeast

Mutation

None

Promoter

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSK260

Plasmid

#129252

Purpose

dual yTRAP, NM fusion to aTF1 reporting on [PSI+] state with mKate2 reporter

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Insert

Sup35 NM yTRAP sensor reporting on mKate2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWKS12

Plasmid

#139419

Purpose

Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised Superfolder GFP marker.

Depositor

James Fraser

Article

Spencer et al Fungal Genet Biol. 2020 May;138:103365.

Insert

Superfolder GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
HsCERK

Plasmid

#118600

Purpose

Expresses ceramide kinase, which catalyzes the phosphorylation of ceramide to form ceramide 1-phosphate

Depositor

Kevin Lynch

Article

Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do

Insert

Homo sapiens ceramide kinase

Use

Tags

FLAG

Expression

Yeast

Mutation

Promoter

GAL1, 10

Available since

Feb. 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSK247

Plasmid

#131148

Purpose

dual yTRAP, Gateway destination vector of aTF2 fusion reporting on mNeonGreen

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

pSUP35, min pCYC1

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB2989

Plasmid

#63636

Purpose

pCfB2989 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.URA3. Additionally it contains a USER cassette.

Depositor

Jerome Maury, Irina Borodina

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

none

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV FHA-ATG12

Plasmid

#27049

Depositor

Jayanta Debnath

Article

Radoshevich et al Cell. 2010 Aug 20. 142(4):590-600.

Insert

autophagy-related 12 (yeast) (Atg12 Mouse)

Use

Retroviral

Tags

HA and flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 13, 2011

Availability

Academic Institutions and Nonprofits only
pAG416GPD-Cerulean-YPT7

Plasmid

#18847

Depositor

Susan Lindquist

Article

Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

YPT7 (YPT7 Budding Yeast)

Use

Tags

Cerulean

Expression

Yeast

Mutation

Promoter

Available since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pCfB2802

Plasmid

#63655

Purpose

pCfB2802 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Jerome Maury, Irina Borodina

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

TEF1 promoter

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLyGo-Kp-2

Plasmid

#163144

Purpose

pLyGo cloning vector for a sequence of interest (LPMO) in K. phaffii. Vector encoding the alfa-amylase signal peptide and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Article

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

AOX1

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB2990

Plasmid

#63645

Purpose

pCfB2990 is a vector for multiple integrations at sites sharing homology with Ty3Cons. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.

Depositor

Jerome Maury, Irina Borodina

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

none

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCTCON2-BXG-2TAG

Plasmid

#158138

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (2 TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment

Use

Tags

Expression

Yeast

Mutation

Cloned in a TAG codon at the first serine residue…

Promoter

GAL1-10

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDDGFP-Leu2d_GgPCFT

Plasmid

#165414

Purpose

expression GgPCFT protein with C terminally tagged GFP-His tag cleavable with TEV protease.

Depositor

Simon Newstead

Article

Parker et al Nature. 2021 May 26. pii: 10.1038/s41586

Insert

GgPCFT (SLC46A1 Chicken, Synthetic)

Use

Tags

GFP-His8

Expression

Yeast

Mutation

Promoter

GAL1

Available since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB2798

Plasmid

#63659

Purpose

pCfB2798 is a vector for multiple integrations at sites sharing homology with Ty4Cons. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Jerome Maury, Irina Borodina

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

TEF1 promoter

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pV1386

Plasmid

#111445

Purpose

Solo vector pV1382 + sgScADE2

Depositor

Gerald Fink

Article

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgScADE2/stop-codon repair

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
721-2µ-staHIS3

Plasmid

#40611

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Insert

HIS3 (HIS3 Budding Yeast)

Use

Tags

HA

Expression

Yeast

Mutation

Promoter

TDH3

Available since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWKS2

Plasmid

#139410

Purpose

Fluoroescent marker for use in Cryptococcus neoformans. Contains a non-codon optimised GFP S65T marker.

Depositor

James Fraser

Article

Spencer et al Fungal Genet Biol. 2020 May;138:103365.

Insert

GFP S65T

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB2800

Plasmid

#63657

Purpose

pCfB2800 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Jerome Maury, Irina Borodina

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

TEF1 promoter

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV FHA-Stop

Plasmid

#27050

Depositor

Jayanta Debnath

Article

Radoshevich et al Cell. 2010 Aug 20. 142(4):590-600.

Insert

autophagy-related 12 (yeast) (Atg12 Mouse)

Use

Retroviral

Tags

HA and flag

Expression

Mammalian

Mutation

changed glycine 141 to stop codon.

Promoter

Available since

Jan. 13, 2011

Availability

Academic Institutions and Nonprofits only