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Plasmid#191093PurposeTo express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methodsDepositorInsertH2B and mScarlet
UseAAVTagsH2B-V5-mScarletExpressionMammalianMutationThe fusion protein was optimized to the Human cod…PromoterCMV and CAGAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPSV39-ugi
Plasmid#186556PurposeBroad host range inducible expression of uracil-DNA glycosylase inhibitor (UGI)DepositorInsertugi
UseTagsExpressionBacterialMutationPromoterLacUV5Available sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-PE
Plasmid#162796PurposeAll-in-one prime editor piggyBac transposonDepositorInsertSpCas9_ H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
UseCRISPR and Synthetic Biology; Piggybac transposonTagsSV40 NLSExpressionMammalianMutationPromoterCAGAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
APEX2-6xHp
Plasmid#214411PurposeLipid droplet localization of APEX2-6xHp for electron microscopyDepositorInsert6x Spastin aa 43-92 (APEX2 Human)
UseTagsAPEX2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mIRFP670nuc-TEV-Xph20-ETWV
Plasmid#133044PurposeExpresses Xph20-ETWV in mammalian cells with nuclear miRFP670 as reporterDepositorInsertXph20-ETWV
UseTagsNLS, TEV protease + TEV cleavage site, and miRFP6…ExpressionMammalianMutationS63KPromoterCAGAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
His6tev CBP HAT (1324-1700)
Plasmid#99340Purposebacterial expression of His tagged mouse CBP HAT domain (1324-1700)DepositorInsertmouse CBP HAT domain (1324-1700) (Crebbp Mouse)
UseTagsHis6tevExpressionBacterialMutationPromoterT7Available sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
H6SUMO-IntC-P53(304-393)
Plasmid#200316Purposebacterial expression of P53 TET-CTD for segmental labelingDepositorInsertp53 (304-393) (TP53 Human)
UseTagsHis6-SUMO and designed intein CExpressionBacterialMutationPromoterT7Available sinceMay 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
H6GB1tevP53(1-303)IntN
Plasmid#200312PurposeBacterial expression of P53 NTAD-DBD for segmental labelingDepositorInsertp53 (TP53 Human)
UseTagsHis6GB1tev and designed intein NExpressionBacterialMutationM133L, V203A, N239Y, N268DPromoterT7Available sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
His6tev CBP BCHZ (1079-1751)
Plasmid#99341PurposeBacterial expression of His tagged mouse CBP HAT core Brd-ZZ (1079-1751)DepositorInsertmouse CBP BRD-ZZ domains (1079-1751) (Crebbp Mouse)
UseTagsHis6tevExpressionBacterialMutationPromoterT7Available sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-CRY2-CreN
Plasmid#26888PurposeExpresses N-terminal fragment of Cre fused to CRY2 for blue light-induced activation of DNA recombination when used with CIBN-CreC. Also expresses mCherry.DepositorUseTagsExpressionMammalianMutationaa 19-104 of CrePromoterCMVAvailable sinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Puro-NCOA7g1 (BB22)
Plasmid#139457PurposeLentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable markerDepositorInsertNCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Puro-NCOA7g2 (BB23)
Plasmid#139458PurposeLentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable markerDepositorInsertNCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-EGFP-NLS-P2AT2A-mCherry-PTS1
Plasmid#87828PurposeHigh-efficient mammalian expression vector for co-expression of EGFP-tagged and mCherry-tagged proteins using P2AT2A. NLS and PTS1 can be replaced by proteins of interest.DepositorInsertsNLS
PTS1
UseTagsEGFP and mCherryExpressionMammalianMutationPromoterCMV promoter, tetracycline operatorAvailable sinceApril 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pES001 MBP-GSK3β-HA-His
Plasmid#154010PurposeExpresses MBP-GSK3β-HA-His (human Glycogen Synthase Kinase 3β as a fusion protein with MBP, HA, and His- tags) in E.coliDepositorInsertGSK3B (GSK3B Human)
UseTagsHA tag, His6, and MBPExpressionBacterialMutationPromoterTacAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-SPdCas9-DNMT3A-2A-Blast
Plasmid#71216PurposedCas9 fused to human DNMT3A catalytic domainDepositorInsertdSpCas9-DNMT3A catalytic domain
UseLentiviralTagsdSpCas9-DNMT3AExpressionMammalianMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWPT-/mEGFP-1T-IRES-mCherry
Plasmid#190190PurposeLentiviral expression vector with altered Kozak sequence to direct EGFP expression. mCherry expression is regulated by an IRES.DepositorInsertsmEGFP
mCherry
UseLentiviralTagsExpressionMutationchanged EGFP Kozak sequence inserting a C>T mo…PromoterEIF1-shortAvailable sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-EGFP-NLS-P2A-mCherry-PTS1
Plasmid#87803PurposeMammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using P2A. NLS and PTS1 can be replaced by proteins of interest.DepositorInsertsNLS
PTS1
UseTagsEGFP and mCherryExpressionMammalianMutationPromoterCMV promoter, tetracycline operatorAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-CMV-puro-2A-BLM
Plasmid#127641PurposeOver-expression of human BLMDepositorInsertBLM (BLM Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/NDUFS3/myc-His
Plasmid#127487PurposeExpresses myc-tagged NDUFS3 in mammalian cellsDepositorInsertNDUFS3 (NDUFS3 Human)
UseTagsmyc-HisExpressionMammalianMutationPromoterCMVAvailable sinceJuly 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/NDUFS2/myc-His
Plasmid#127486PurposeExpresses myc-tagged NDUFS2 in mammalian cellsDepositorInsertNDUFS2 (NDUFS2 Human)
UseTagsmyc-HisExpressionMammalianMutationPromoterCMVAvailable sinceJuly 17, 2019AvailabilityAcademic Institutions and Nonprofits only