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Plasmid#134470PurposeHuman expression vector for SaCas9-HF variant.DepositorInsertStaphylococcus aureus Cas9 nuclease (SauCas9) with high genome-wide specificity
UseCRISPRTagsSV40NLS-3xFLAGExpressionMammalianMutationR245A, N413A, N419A, R654APromoterCAGAvailable sinceNov. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-Fbx15-cont2
Plasmid#27081DepositorInsertFBXO15 (FBXO15 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-Xpress-GBF1
Plasmid#206417PurposeMammalian expression of hGBF1DepositorInsertGBF1 (GBF1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
p2T-cmv-SpCas9-TadDE-BlastR
Plasmid#193850PurposeExpress TadDE in mammalian cells with BlastR selection, Tol2 integrationDepositorInsertTadDE
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIG PIK3CA E545K
Plasmid#73055PurposeHuman PIK3CA E545KDepositorInsertPIK3CA (PIK3CA Human)
UseTagsExpressionMammalianMutationE545KPromoterAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCIG PIK3CA Wildtype
Plasmid#73056PurposeHuman PIK3CA WildtypeDepositorInsertPIK3CA Wildtype (PIK3CA Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
Flag-cIAP2/pRK5
Plasmid#27973DepositorInsertCIAP2 (BIRC3 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHS1861
Plasmid#205974PurposeFLAG-SV40 NLS-Cas8-HNH from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)DepositorInsertCas8-HNH
UseTagsFLAG-SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delZn3
Plasmid#173936PurposeBacterial expression of PARP1 lacking Zn3 domainDepositorInsertPARP1delZn3 (PARP1 Human)
UseTags6xHisExpressionBacterialMutationDeletion of residues 214-373; V762APromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delBRCT
Plasmid#173938PurposeBacterial expression of PARP1 lacking BRCT domainDepositorInsertPARP1delBRCT (PARP1 Human)
UseTags6xHisExpressionBacterialMutationDeletion of residues 374-520; V762APromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG::Cytbow
Plasmid#158995PurposeMulticolor cytoplasmic cell labeling with a genome-integrating piggyBac transposon vector (randomly expressing tdTomato, mTurquoise2 or mEYFP upon Cre recombination)DepositorInsertCytbow
UseTagsThe default expressed EBFP2 is fused to H2BExpressionMammalianMutationPromoterCAGAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2812 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9
Plasmid#84240PurposeExpresses GA-inducible VPR-Sp dCas9DepositorInsertsGAI-tagBFP-Sp dCas9
GID1-VPR
UseCRISPR and Synthetic Biology; PiggybacTags2xNLS (SV40), GAI, GID1, HA tag, IRES-Puro, and t…ExpressionMammalianMutationPromoterCAG and PGKAvailable sinceNov. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
VN-beta Synuclein
Plasmid#89483Purposeexpresses human beta Synuclein fused to the N-terminal part of VenusDepositorInsertSNCB (SNCB Human)
UseTagsN-terminal part of VenusExpressionMammalianMutationPromoterCMVAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delCAT
Plasmid#173942PurposeBacterial expression of PARP1 lacking CAT domainDepositorInsertPARP1delCAT (PARP1 Human)
UseTags6xHisExpressionBacterialMutationDeletion of residues 661-1014PromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti HRE mutant-Luc pGK Hygro
Plasmid#118707Purposereporter plasmid, HIF-responsive promoter (3xHRE) mutant- fused to firefly luciferaseDepositorInsertHREmutant-Luc
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMH-SFB-BRCA1
Plasmid#99394PurposeMammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged BRCA1DepositorInsertBRCA1 (BRCA1 Human)
UseTagsSFB (S protein-FLAG-Streptavidin binding peptide)ExpressionMammalianMutationPromoterAvailable sinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMP71-mIL15-IL12rb1-sushi-il12rb2
Plasmid#174606PurposeExpresses a constitutively activated murine IL-12 receptor tagged with extracellular myc and flag tagsDepositorInsertExpresses a constitutively activated murine IL-12 receptor tagged with extracellular myc and flag tags
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1deltaART
Plasmid#174804PurposeBacterial expression of an inactive PARP1 mutant lacking the ART subdomainDepositorInsertPARP1deltaART (PARP1 Human)
UseTags6xHisExpressionBacterialMutationDeletion of residues 785-1014PromoterAvailable sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delWGR
Plasmid#173940PurposeBacterial expression of PARP1 lacking WGR domainDepositorInsertPARP1delWGR (PARP1 Human)
UseTags6xHisExpressionBacterialMutationDeletion of residues 521-660; V762APromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delZn1
Plasmid#173933PurposeBacterial expression of PARP1 lacking Zn1 domainDepositorInsertPARP1delZn1 (PARP1 Human)
UseTags6xHisExpressionBacterialMutationDeletion of residues 1-96; V762APromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only