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Plasmid#184910PurposeTemplate to generate via PCR a single gRNA for expression in S. cerevisiaeDepositorInsertgRNA scaffold
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
MmSphk1
Plasmid#118599PurposeExpresses sphingosine kinase 1, which catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate in miceDepositorInsertMus musculus sphingosine kinase 1
UseTagsFLAGExpressionYeastMutationPromoterGAL1, 10Available sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pSK234
Plasmid#131149Purposedual yTRAP, Gateway destination vector of aTF2 fusion reporting on mKate2DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionYeastMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGZ110 (pFA6a-3FLAG-MNase-TRP1)
Plasmid#70233PurposeChEC-tagging vector encoding a 3xFLAG tag and aa 83-231 of Mnase in pFA6a-3HA-Trp1 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitope tagDepositorInsert3xFLAG tag and aa 83-231 of Mnase
UseYeast genomic targetingTagsExpressionMutationPromoterAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
nuc-yHS1
Plasmid#159166PurposeNuclear labile heme reporterDepositorInsertnuc-yHS1
UseTagsSV40 NLSExpressionYeastMutationPromoterGPDAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
UG75-ADH1-CDS1-3HA
Plasmid#198596PurposeCDS1 overexpression. Diverts glycerolipid synthesis pathway towards phospholipids. Uses antibiotic resistance marker hphMX6.DepositorInsertCDS1 (CDS1 Budding Yeast)
UseTags3HAExpressionYeastMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
UG75-ADH1-DGK1-3HA
Plasmid#198595PurposeDGK1 overexpression. Promotes the formation of phosphatidate from diacylglycerol. Uses antibiotic resistance marker hphMX6.DepositorInsertDGK1 (DGK1 Budding Yeast)
UseTags3HAExpressionYeastMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
UG75-ADH1-PAH1-3HA
Plasmid#198597PurposePAH1 overexpression. Promotes the formation of diacylglycerol from phosphatidate. Uses antibiotic resistance marker hphMX6.DepositorInsertPAH1 (PAH1 Budding Yeast)
UseTags3HAExpressionYeastMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMJA181
Plasmid#128539PurposeExpresses anti-Prunus dulcis scFv Ab+BirA tagDepositorInsertscFv Ab clone PD1F6 plus BirA tag
UseTagsExpressionYeastMutationPromoterAOX1Available sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPT-PCR C2
Plasmid#141247PurposePCR cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 2DepositorInsertMitochondrial Genome of Phaeodactylum tricornutum
UseSynthetic Biology; Diatom expressionTagsExpressionBacterial and YeastMutationRepeat region removed, additional minor mutations…PromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCfB2803
Plasmid#63646PurposepCfB2803 is a vector for multiple integrations at sites sharing homology with Ty4Cons. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoternoneAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pV2A-T
Plasmid#153022PurposeSingle-plasmid multi-gene expression system suitable for fungi. The obtained polycistronic gene is expressed under the control of a TetON inducible promoterDepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceOct. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
YEpURA-CUP1-HBTubiquitin
Plasmid#26900DepositorInsertHBT-tagged ubiquitin
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCfB2796
Plasmid#63641PurposepCfB2796 is a vector for multiple integrations at sites sharing homology with Ty4Cons. The selective marker is Kl.URA3. Additionally it contains a USER cassette.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoternoneAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB2804
Plasmid#63644PurposepCfB2804 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoternoneAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pML104-kanR1136
Plasmid#162040PurposeExpresses Cas9 and contains kanR1136 guide which targets the kanR geneDepositorInsertkanR1136 guide
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB2797
Plasmid#63639PurposepCfB2797 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.URA3. Additionally it contains a USER cassette.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoternoneAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB2875
Plasmid#63640PurposepCfB2875 is a vector for multiple integrations at sites sharing homology with Ty3Cons. The selective marker is Kl.URA3. Additionally it contains a USER cassette.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoternoneAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
1000.pCCLsin.cPPT.hPGK.Myt1L.MIRT124_x4 Xma_lost.WPRE
Plasmid#67293PurposeInduce human iN conversion from fibroblastDepositorInsertMyt1L (Myt1l Mouse)
UseLentiviralTagsExpressionMammalian and YeastMutationPromoterPGKAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only