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pLV-EF1a-GFP ArgSen delta Rad23b UbL

Plasmid

#184703

Purpose

Lentiviral transfer plasmid for expression of GFP ArgSen delta Rad23b UbL

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

GFP ArgSen delta Rad23b UbL

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pBoBi-hTOMM20-C-PEN2

Plasmid

#183647

Purpose

Lentiviral expression of a mitochondrion-residential PEN2.

Depositor

Sheng-cai Lin

Article

Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

Insert

PEN2 and TOMM20 (PSENEN, TOMM20 Human)

Use

Lentiviral

Tags

Expression

Mutation

WT

Promoter

CMV

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-XRN1__PRKCE

Plasmid

#116846

Purpose

Lentiviral expression of XRN1__PRKCE fusion

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

XRN1__PRKCE

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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MEK1-pcw107-V5

Plasmid

#64650

Purpose

(control) when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

MAP2K1 (MAP2K1 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

PGK

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
pHR-ZIP(RK)

Plasmid

#27136

Depositor

Ron Vale

Article

Yudushkin et al Proc Natl Acad Sci U S A. 2010 Dec 21;10

Insert

CD3zeta, eGFP, mCherry and ZAP70 (residues 1-259) (ZAP70, Cd247 Mouse, Human)

Use

Lentiviral

Tags

eGFP and mCherry

Expression

Mammalian

Mutation

Arginines in the SH2 domains mutated to lysines (…

Promoter

Available since

April 12, 2011

Availability

Academic Institutions and Nonprofits only

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TFORF3299

Plasmid

#144775

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

MEIS2 (MEIS2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNotch1#1/Cre

Plasmid

#193223

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Notch1 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgNotch1#1 (Notch1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF0732

Plasmid

#141675

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

PROX2 (PROX2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-sgFbxw7#1/Cre

Plasmid

#173635

Purpose

Expresses a Fbxw7-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Fbxw7 (Fbxw7 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgFat1#1/Cre

Plasmid

#173633

Purpose

Expresses a Fat1-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Fat1 (Fat1 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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RIPK1 gRNA (BRDN0001146967)

Plasmid

#76534

Purpose

3rd generation lentiviral gRNA plasmid targeting human RIPK1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RIPK1 (RIPK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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RIPK1 gRNA (BRDN0001149147)

Plasmid

#76535

Purpose

3rd generation lentiviral gRNA plasmid targeting human RIPK1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RIPK1 (RIPK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCLX-UBI-GFP

Plasmid

#27245

Depositor

Patrick Salmon

Article

Salmon et al Methods Mol Biol. 2013;945:417-48. doi:

Insert

GFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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plenti-UbcP-HA-YWAA-pGK-HYG

Plasmid

#107379

Purpose

Lentiviral expression of HA-YWAA

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

cereblon (CRBN Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

CRBN-Y383A/W385A

Promoter

UbcP

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pLp6.3-EglN3-H196A

Plasmid

#79116

Purpose

Lentiviral expression of EglN3-H196A

Depositor

Susanne Schlisio

Insert

EglN3 catalytic dead mutant (EGLN3 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

catalytic dead mutant: H196A

Promoter

CMV

Available since

July 7, 2016

Availability

Academic Institutions and Nonprofits only

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AKT1 gRNA (BRDN0001149266)

Plasmid

#75501

Purpose

3rd generation lentiviral gRNA plasmid targeting human AKT1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AKT1 (AKT1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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RAF1 gRNA (BRDN0001145852)

Plasmid

#76707

Purpose

3rd generation lentiviral gRNA plasmid targeting human RAF1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RAF1 (RAF1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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FLAG-Mkk7-JNK2 fusion-pcw107

Plasmid

#64618

Purpose

Mkk7-JNK2 fusion - barcoded activating construct of JNK pathway

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

Map2k7 linker MAPK9

Use

Lentiviral

Tags

FLAG

Expression

Mutation

mouse-human fusion

Promoter

PGK

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
NADK2 gRNA (BRDN0001487085)

Plasmid

#78093

Purpose

3rd generation lentiviral gRNA plasmid targeting human NADK2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NADK2 (NADK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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Fgf4enhLV (FpG12)

Plasmid

#69444

Purpose

positive control plasmid for lentiviral reporter expression in ESCs

Depositor

Lisa Dailey

Article

Murtha et al Nat Methods. 2014 May;11(5):559-65. doi:

Inserts

minimal FGF4 promoter
EGFP
Ubiquitin promoter
Hygromycin resistance
FGF4 enhancer (Fgf4 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 5, 2016

Availability

Academic Institutions and Nonprofits only

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