Fgf4enhLV (FpG12)
(Plasmid
#69444)
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Purposepositive control plasmid for lentiviral reporter expression in ESCs
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 69444 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneFUW
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Backbone manufacturerLois and Baltimore (ADDGENE # 14882)
- Backbone size w/o insert (bp) 9206
- Total vector size (bp) 11906
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Modifications to backboneinserted FGF4 enhancer and minimal FGF4 promoter upstream of GFP coding sequences. Inserted human Ubiquitin promoter upstream of Hygromycin resistance gene. cloning details described in Murtha et al Nat Meth 2014
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Vector typeMammalian Expression, Lentiviral
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)Stbl2
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameminimal FGF4 promoter
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SpeciesM. musculus (mouse)
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Insert Size (bp)199
Gene/Insert 2
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Gene/Insert nameEGFP
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Insert Size (bp)720
Gene/Insert 3
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Gene/Insert nameUbiquitin promoter
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SpeciesH. sapiens (human)
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Insert Size (bp)1192
Gene/Insert 4
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Gene/Insert nameHygromycin resistance
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Insert Size (bp)1038
Gene/Insert 5
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Gene/Insert nameFGF4 enhancer
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SpeciesM. musculus (mouse)
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Insert Size (bp)249
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Entrez GeneFgf4 (a.k.a. Fgf-4, Fgf7a, Fgfk, HBGF-4, Hst1, Hstf-1, KS3, hst, hst-1, kFGF)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that several discrepancies were found between Addgene's quality control and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Fgf4enhLV (FpG12) was a gift from Lisa Dailey (Addgene plasmid # 69444 ; http://n2t.net/addgene:69444 ; RRID:Addgene_69444) -
For your References section:
FIREWACh: high-throughput functional detection of transcriptional regulatory modules in mammalian cells. Murtha M, Tokcaer-Keskin Z, Tang Z, Strino F, Chen X, Wang Y, Xi X, Basilico C, Brown S, Bonneau R, Kluger Y, Dailey L. Nat Methods. 2014 May;11(5):559-65. doi: 10.1038/nmeth.2885. Epub 2014 Mar 23. 10.1038/nmeth.2885 PubMed 24658142