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Plasmid#187337PurposedCas9 effector fusion for manipulating transcriptionDepositorInsertdCas9-3XFLAG-N+Z+F-T2A-tagBFP-P2A-Blast
UseCRISPR and LentiviralTags3XFLAGExpressionMammalianMutationPromoterpEF1aAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
Plenti-CMV-MCS-RFP-SV-puro
Plasmid#109377PurposeThis plasmid expresses dsRed2 and puromycin resistanceDepositorTypeEmpty backboneUseLentiviralTagsdsRed2ExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_T5-ARG1
Plasmid#106476PurposeExpresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli Nissle 1917DepositorInsertARG1
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT5Available sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG mito-mEos2
Plasmid#168515PurposeExpression of photoconvertible mEos2 targted to mitochondrial matrix to allow tracking of individual mitochondria and measurement of fission-fusion rateDepositorInsertmito-mEos2
UseTagsmEos2ExpressionMammalianMutationPromoterCAGAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-M13-TEV-C-P2A-TdTomato
Plasmid#92391Purposeproduces AAV expressing Cal-light M13-TevC component and TdTomatoDepositorInsertM13-TEV-C-P2A-TdTomato
UseAAV and Synthetic BiologyTagsTdTomatoExpressionMammalianMutationPromoterhuman synapsinAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
L13-Arl13bGFP
Plasmid#40879Purpose3rd generation transfer vectorDepositorInsertArl13b (Arl13b Mouse)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRK5-EGFP-Tau AP
Plasmid#46905Purposeexpresses EGFP tagged Tau AP in mammalian cellsDepositorInsertTau AP (MAPT Human)
UseTagsEGFPExpressionMammalianMutationall 14 S/P or T/P amino acid residues (T111, T153…PromoterCMVAvailable sinceJuly 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Spike
Plasmid#173921PurposeExpresses the original SARS-Cov-2 Spike Protein under a CMV PromoterDepositorInsertSARS-2 Spike Protein (S Human, Synthetic, SARS-COV-2)
UseTagsno tags, native proteinExpressionBacterial and MammalianMutationPromoterstandard CMVAvailable sinceApril 11, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGreenIIM DR5v2-ntdTomato/DR5-n3GFP
Plasmid#61628PurposeExpresses nuclear tdTomato from auxin-dependent DR5v2 (TGTCGG) promoter and nuclear 3xGFP from the DR5 (TGTCTC) promoterDepositorInsertsntdTomato
n2xEGFP
UseTagsExpressionPlantMutation2 copies of EGFP with N-terminal SV40 NLSPromoterAuxin-dependent DR5 promoter containing 9 reverse…Available sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHCMV-VSVgSS-SNAP-TM
Plasmid#207319PurposeFunctional incorporation of SNAP-tag into envelope of virionsDepositorInsertSNAP-tag
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
AbVec2.0-mIghg1
Plasmid#127158PurposeExpression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG1 isotypeDepositorInsertIghg1 (Ighg1 Mouse)
UseTagsExpressionMammalianMutationPromoterhCMV IE1 gene promoterAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTHSSe_52
Plasmid#109250PurposeColE1 BbsI cloning vectorDepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromoterPJ23105Available sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
p45sheLL
Plasmid#37323DepositorInsertHPV45L1+L2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pKM512
Plasmid#140192PurposeFor curing of Orbit-integrated plasmids and associated drug markerDepositorInsertszeocin resistance gene
Bxb1gp47 (directionality factor)
UseTagsExpressionBacterialMutationCloned in gp47 directinality factor in place of R…PromoterEM7 and PtetAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTHSSe_42
Plasmid#109240PurposepSC101 BbsI cloning vectorDepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromoterPJ23105Available sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV2-ITR-pEFS-NLS_hfCas13d(RfxCas13d_N2V8)_FLAG_NLS-pA-U6-DR-Pcsk9-sg5-DR-Pcsk9-sg6-DR-AAV2-ITR
Plasmid#191795Purposevector for encoding and AAV packaging a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by EFS promoter, Pcsk9-target guide RNAs compatible with RfxCas13d driven by hU6DepositorInserthumanized hfCas13d
UseAAV and CRISPRTagsExpressionMammalianMutationA134V, A140V, A141V, A143VPromoterEFS, hU6Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUB-GFP
Plasmid#11155PurposeMammalian expression vector for expression of GFP (Ubiquitin C promoter)DepositorUseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCMV-2NLS-Cas9-6XMS2
Plasmid#166033PurposeExpression of 6X MS2 stem loops fused SpCas9 mRNA for packaging of SpCas9 mRNA within lentiviral particles.DepositorInsertSpCas9
UseCRISPR and LentiviralTagsExpressionMutationPromoterCMVAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Anti-6xHis [N144/14R]
Plasmid#177500PurposeMammalian Expression Plasmid of anti-6xHis. Derived from hybridoma N144/14.DepositorInsertanti-6xHis recombinant mouse monoclonal antibody
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFORF1329
Plasmid#143789PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTFAP2A (TFAP2A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only