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pICE-EGFP-FLAG-Ku70siR-WT

Plasmid

#46961

Purpose

Plasmid for constitutive or doxycycline-inducible expression of wild-type human Ku70 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

Depositor

Steve Jackson

Article

Britton et al J Cell Biol. 2013 Jul 29.

Insert

human Ku70 (XRCC6 Human)

Use

Tags

GFP-FLAG

Expression

Mammalian

Mutation

Promoter

CMV Tet

Available since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-ArchT-GFP

Plasmid

#63141

Purpose

AAV-mediated expression of ArchT-GFP under the Syn promoter. Using hGHpA signal.

Depositor

Edward Boyden

Article

Han et al Front Syst Neurosci. 2011;5:18. Epub 201

Insert

ArchT-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

Promoter

human synapsin promoter

Available since

May 11, 2016

Availability

Academic Institutions and Nonprofits only

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pLPCX-Cx43-FS-IRES-GFP

Plasmid

#65438

Purpose

Mammalian expression of frameshift mutated Cx43 and EGFP separately driven by the ECMV IRES. For transfection or retroviral production

Depositor

Trond Aasen

Article

Salat-Canela et al Cell Commun Signal. 2014 May 8;12:31. do

Insert

Cx43 (GJA1 Human)

Use

Retroviral

Tags

IRES-GFP

Expression

Mammalian

Mutation

frameshift (FS) mutation 35delA

Promoter

CMV

Available since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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pPDS1-mEGFP-IRES-puro2b

Plasmid

#26464

Depositor

Daniel Gerlich

Article

Held et al Nat Methods. 2010 Sep . 7(9):747-54.

Insert

pPDS1 (PTTG1 Human)

Use

Tags

mEGFP

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Dec. 1, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/Puro/FA/GFP-LL5BIP

Plasmid

#112829

Purpose

transient/stable overexpression

Depositor

Tomasz Prószyński

Article

Niewiadomski et al Matters v. 2, n. 11 (Nov 17, 2016)

Insert

LL5BIP (2410002F23Rik Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-Axin-IRES-GFP

Plasmid

#14716

Depositor

Tannishtha Reya

Article

Reya et al Nature. 2003 May 22. 423(6938):409-14.

Insert

Axin (Axin1 Mouse)

Use

Retroviral

Tags

IRES-GFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only
pTol2_(-3kb)nrg1_typeII:GFP-CAAX

Plasmid

#199394

Purpose

pDEL63; transgenic construct to express GFP-CAAX under nrg1 type II regulatory region control

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

(-3kb)nrg1 type II
GFP-CAAX
afp_3'_UTR

Use

Tol2 gateway destination vector

Tags

Expression

Mutation

Promoter

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Connexin43-GFP-APEX

Plasmid

#44440

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103

Insert

Connexin43 -EGFP - pea APEX

Use

Tags

Expression

Mammalian

Mutation

K14D, W41F, E112K mutations on APEX

Promoter

CMV

Available since

May 14, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-WHaloCaMP1a-EGFP

Plasmid

#205309

Purpose

Production of AAV particles encoding WHaloCaMP1a-EGFP from the CaMKII promoter

Depositor

Eric Schreiter

Article

Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

Insert

WHaloCaMP1a-EGFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAS_4xMx PylT C41CA_GFP 150TAG

Plasmid

#140017

Purpose

Plasmid with 4xMxPylT C41CA cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

sfGFP

Use

Tags

Expression

Mammalian

Mutation

150TAG

Promoter

EF1

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO5-SgHottip-GFP-CRISPRa

Plasmid

#134990

Purpose

dCas9-mediated activation of HOTTIP in mammalian cells

Depositor

Suming Huang

Article

Luo et al Cancer Cell. 2019 Dec 9;36(6):645-659.e8

Insert

HOTTIP (HOTTIP Human)

Use

Lentiviral

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

Promoter

U6

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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attB-GFP-Poly(A)‐NeoR

Plasmid

#65526

Purpose

NeoR containing plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

attB-GFP

Use

Mouse Targeting; Bxb1

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV Gsk3 sgRNA/GFP

Plasmid

#112733

Purpose

Gsk3b targeting gRNA cloned into px552 (SpGuide) plasmid.

Depositor

Martin Beaulieu

Article

Khlghatyan et al Front Mol Neurosci. 2018 Apr 12;11:119.

Insert

GFP

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/Puro/FA/GFP-N--Grb2-5

Plasmid

#112840

Purpose

transient/stable overexpression

Depositor

Tomasz Prószyński

Article

Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

Insert

Grb2 (Grb2 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Full length Grb2

Promoter

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV KSR1 IRES GFP

Plasmid

#25973

Depositor

Rob Lewis

Article

Kortum et al Mol Cell Biol. 2004 May . 24(10):4407-16

Insert

Kinase suppressor of Ras 1 (Ksr1 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2010

Availability

Academic Institutions and Nonprofits only
pLenti-STAT3-linker-TagGFP2

Plasmid

#121427

Purpose

WT STAT3 inserted using Infusion Cloning into MluI and EcoRI digested pLenti-Origene-Nrf21.

Depositor

Ovijit Chaudhuri, Stanley Qi

Article

Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

Insert

STAT3-linker-TagGFP2

Use

Tags

TagGFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P41nmt1-GFP

Plasmid

#39290

Depositor

John Pringle, Jurg Bahler

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

nmt1 promoter (nmt1 Fission Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Expression

Mutation

-1163 to +6 of nmt1 locus with a 4bp deletion (TA…

Promoter

Available since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO GFP HSPH3

Plasmid

#19482

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

HSPH3 (HSPA4L Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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PITX3-2A-eGFP-PGK-Puro

Plasmid

#31943

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

Insert

PITX3 (PITX3 Human)

Use

Targeting donor

Tags

eGFP

Expression

Mutation

Promoter

-

Available since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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B3GALT5_pLENTI-CAG-IRES-GFP

Plasmid

#177006

Purpose

Mammalian lentiviral expression vector encoding B3GALT5

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

B3GALT5 (B3GALT5 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CAG

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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