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Plasmid#77254Purpose3rd generation lentiviral gRNA plasmid targeting human GUCY2FDepositorInsertGUCY2F (GUCY2F Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
CAMK4 gRNA (BRDN0001145862)
Plasmid#77226Purpose3rd generation lentiviral gRNA plasmid targeting human CAMK4DepositorInsertCAMK4 (CAMK4 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
ANKK1 gRNA (BRDN0001149290)
Plasmid#76035Purpose3rd generation lentiviral gRNA plasmid targeting human ANKK1DepositorInsertANKK1 (ANKK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-multi-Puro (pRW1441)
Plasmid#216217PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi construct with Puromycin resistanceDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP
Plasmid#118156PurposeCatalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.DepositorInsertKRAB-dCas9-P2A-EGFP
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterEF1a core and U6Available sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-multi-Zeo (pRW1445)
Plasmid#216218PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi construct with Zeocin resistanceDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAZ-SK
Plasmid#73962Purposeself-killing gRNA plasmid pMAZ-SKDepositorInsertgRNA
UseTagsExpressionBacterialMutationPromoterpTetAvailable sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro U6 sgRNA BfuAI large stuffer
Plasmid#52628Purposelentiviral U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites, improved cassette cloning efficiencyDepositorInsertKanamycin cassette
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
PX398 Nitratifractor salsuginis str DSM 16511 Cas9
Plasmid#68334PurposeNitratifractor salsuginis str DSM 16511 Cas9DepositorInsertNsCas9
UseCRISPRTagsHA and NLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCSDC2-donor
Plasmid#113813PurposeCRISPR donor plasmid to tag human transcription factor CSDC2 with GFPDepositorInsertCSDC2 homology arms flanking EGFP-IRES-Neo cassette (CSDC2 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
GEARBOCS
Plasmid#218181PurposeCRISPR tool to target astrocytesDepositorTypeEmpty backboneUseAAV and CRISPRTagsExpressionMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pZNF215-donor
Plasmid#113804PurposeCRISPR donor plasmid to tag human transcription factor ZNF215 with GFPDepositorInsertZNF215 homology arms flanking EGFP-IRES-Neo cassette (ZNF215 Human)
UseCRISPRTagsExpressionMutationrs2239733, rs2239732, hg38:11:6956348:T>G, rs1…PromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF41-donor
Plasmid#112375PurposeCRISPR donor plasmid to tag human transcription factor ZNF41 with GFPDepositorInsertZNF41 homology arms flanking EGFP-IRES-Neo cassette (ZNF41 Human)
UseCRISPRTagsExpressionMutationhg38:X:47447496:G>T, hg38:X:47448269:C>A, h…PromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pELF4-donor
Plasmid#112371PurposeCRISPR donor plasmid to tag human transcription factor ELF4 with GFPDepositorInsertELF4 homology arms flanking EGFP-IRES-Neo cassette (ELF4 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZBTB26-donor
Plasmid#112356PurposeCRISPR donor plasmid to tag human transcription factor ZBTB26 with GFPDepositorInsertZBTB26 homology arms flanking EGFP-IRES-Neo cassette (ZBTB26 Human)
UseCRISPRTagsExpressionMutationhg38:9:122919395:A>G, hg38:9:122919458:T>G,…PromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF84-donor
Plasmid#112330PurposeCRISPR donor plasmid to tag human transcription factor ZNF84 with GFPDepositorInsertZNF84 homology arms flanking EGFP-IRES-Neo cassette (ZNF84 Human)
UseCRISPRTagsExpressionMutationrs623100, hg38:12:133058872:T>C, hg38:12:13305…PromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF75A-donor
Plasmid#112307PurposeCRISPR donor plasmid to tag human transcription factor ZNF75A with GFPDepositorInsertZNF75A homology arms flanking EGFP-IRES-Neo cassette (ZNF75A Human)
UseCRISPRTagsExpressionMutationhg38:16:3317462:A>T, hg38:16:3317715:C>T, h…PromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF764-donor
Plasmid#112298PurposeCRISPR donor plasmid to tag human transcription factor ZNF764 with GFPDepositorInsertZNF764 homology arms flanking EGFP-IRES-Neo cassette (ZNF764 Human)
UseCRISPRTagsExpressionMutationhg38:16:30555233:G>A, hg38:16:30555234:C>G,…PromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP
Plasmid#67991PurposeCRISPR gRNA expression vector with an improved l scaffold and puro/BFP markers without WPREDepositorInsertU6gRNA cassette with an improved scaffold, PGKpuro2ABFP cassette
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKLV2.2-h7SKgRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W
Plasmid#72666PurposeLentiviral dual CRISPR gRNA expression vector (human 7SK and human U6 promoters)DepositorInserth7SKgRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only