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Plasmid#165910PurposeBlasticidin resistant 5xUAS sfGFP Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable UAS Response Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBSKΔB-TRE-dCas9-EGFP
Plasmid#62347PurposePlasmid for tet-inducible expression of dCas9-EGFP in mammalian cellsDepositorInsertdCas9-EGFP
UseCRISPRTagsHA and SV40 NLSExpressionMammalianMutationPromoterTREAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_nucLAMA-G97 IRES EGFP
Plasmid#136620PurposePlasmid encoding nuclear localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFPDepositorInsertSNAP-Tag-LAMA-G97-NLS (IRES EGFP)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pACT1:Cas9-GFP, U6:sgUPRT
Plasmid#122853PurposeExpresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum UPRT geneDepositorInsertsCas9-GFP
U6-sgUPRT
UseCRISPR; Cas9-gfp plasmid for genome editing of cr…TagseGFPExpressionMutationPromoterCryptosporidium parvum U6 and Cryptosporidium par…Available sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-EGFP-2A-HA-Nrxn3-alpha- 2(-)/3(+)/4(+)/6(+)
Plasmid#184334PurposeExpression of mouse Nrx3-alpha lacking insertion at the alternative splice site 4 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,2,3 and 6.DepositorInsertNrxn3-alpha (Nrxn3 Mouse)
UseTagsHA-tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
ATF4 7: 5’3’ATF4.GFP
Plasmid#21854DepositorInsertATF4 (Atf4 Mouse)
UseTagsEGFPExpressionMammalianMutationVector derived viral termination and poly-adenyla…PromoterAvailable sinceSept. 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pXGFPC-5 Pxyl-dcas9 (Spa)
Plasmid#133318Purposeexpresses dcas9 (Streptococcus pasteurianus); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistanceDepositorInsertdcas9 (Streptococcus pasteurianus)
UseUnspecifiedTagsExpressionMutationPromoterxyloseAvailable sinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP VAMP7 (102-220)
Plasmid#42314DepositorInsertVAMP7 Coiled Coil domain (102-220) (VAMP7 Human)
UseTagsEGFPExpressionMammalianMutationΔ1-102PromoterCMVAvailable sinceMarch 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-GFP(S65T)-TRP1
Plasmid#41597DepositorInsertTRP1 (TRP1 Budding Yeast)
UseYeast genomic targetingTagsGFP (S65T) and yeast ADH1 terminatorExpressionMutationPromoterAvailable sinceJan. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso1_1093-1317_W
Plasmid#147892PurposeMammalian Expression of HsNot1iso1_1093-1317 *Please note that this plasmid encodes amino acids 1093-1322 (not 1093-1317 as the name suggests).DepositorInsertHsNot1iso1_1093-1317 (CNOT1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso1_1318-1605_W
Plasmid#147893PurposeMammalian Expression of HsNot1iso1_1318-1605DepositorInsertHsNot1iso1_1318-1605 (CNOT1 Human)
UseTagsExpressionMammalianMutation1 silent mutation compared to the sequence of iso…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-IRES-hrGFP
Plasmid#107549Purposehigh transduction efficiency AAV-mediated synapsin promoter-dependent expression IRES-hrGFPDepositorTypeEmpty backboneUseAAVTagsExpressionMutationPromoterAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-Bi-gePSI-pCamk2a-AcGFP
Plasmid#133732PurposeExpresses both the gePSI-α-chain and the gePSI-β-chain under control of the inducible bi-directional TRE3G promoter. Expresses constitutively AcGFP under control of a Camk2a promoter.DepositorInsertsgePSI-β-chain
gePSI-α-chain
AcGFP1
UseAAVTagsmurine ornithine decarboxylase - degron (ODC36)ExpressionMammalianMutationPromoterpCamk2a and pTRE3G-BiAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
[UBC][NRE Pum][eGFP]
Plasmid#97192PurposeThe wild-type Pum, fused to eGFP, and behind the ubiquitin promoter.DepositorInsertwild type Pumilio homology domain
UseSynthetic BiologyTagseGFPExpressionMammalianMutationPromoterUBC (ubiquitin C)Available sinceNov. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEST-hMeCP2-GFP-R270X
Plasmid#48079PurposeMammalian expression of GFP tagged MeCP2-R270XDepositorInsertMethyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
UseTagsGFPExpressionMammalianMutationexpresses AT-Hook R270XPromoterCMVAvailable sinceApril 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
DsRed2_pLENTI-CAG-IRES-GFP
Plasmid#177010PurposeMammalian lentiviral expression vector encoding DsRed2DepositorInsertDsRed2
UseLentiviralTagsExpressionMutationPromoterCAGAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB (TIREVOL)-sfGFP
Plasmid#189675Purposetitratable expression of sfGFP in bacteriaDepositorInsertsfGFP
UseTagsHis-T7-EKExpressionBacterialMutationPromoterpBADAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pICE-EGFP-FLAG-Ku70siR-WT
Plasmid#46961PurposePlasmid for constitutive or doxycycline-inducible expression of wild-type human Ku70 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.DepositorInserthuman Ku70 (XRCC6 Human)
UseTagsGFP-FLAGExpressionMammalianMutationPromoterCMV TetAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-ArchT-GFP
Plasmid#63141PurposeAAV-mediated expression of ArchT-GFP under the Syn promoter. Using hGHpA signal.DepositorInsertArchT-GFP
UseAAVTagsGFPExpressionMammalianMutationPromoterhuman synapsin promoterAvailable sinceMay 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLPCX-Cx43-FS-IRES-GFP
Plasmid#65438PurposeMammalian expression of frameshift mutated Cx43 and EGFP separately driven by the ECMV IRES. For transfection or retroviral productionDepositorInsertCx43 (GJA1 Human)
UseRetroviralTagsIRES-GFPExpressionMammalianMutationframeshift (FS) mutation 35delAPromoterCMVAvailable sinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only