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Plasmid#135493PurposeMammalian expression of VN-fused and myc-tagged HLA-A*02:01 (D61P mutant)DepositorInsertHLA-A*02:01 (HLA-A Human)
UseTagsExtracellular c-myc epitope tag, Signal peptide f…ExpressionMammalianMutationD61PPromoterCMVAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-A2-VN (V67P)
Plasmid#135494PurposeMammalian expression of VN-fused and myc-tagged HLA-A*02:01 (V67P mutant)DepositorInsertHLA-A*02:01 (HLA-A Human)
UseTagsExtracellular c-myc epitope tag, Signal peptide f…ExpressionMammalianMutationV67PPromoterCMVAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-A2-VN (Y84P)
Plasmid#135495PurposeMammalian expression of VN-fused and myc-tagged HLA-A*02:01 (Y84P mutant)DepositorInsertHLA-A*02:01 (HLA-A Human)
UseTagsExtracellular c-myc epitope tag, Signal peptide f…ExpressionMammalianMutationY84PPromoterCMVAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-A2-VN (E166P)
Plasmid#135498PurposeMammalian expression of VN-fused and myc-tagged HLA-A*02:01 (E166P mutant)DepositorInsertHLA-A*02:01 (HLA-A Human)
UseTagsExtracellular c-myc epitope tag, Signal peptide f…ExpressionMammalianMutationE166PPromoterCMVAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
Raichu-OsRac1 DN
Plasmid#133263PurposeDominant-negative (DN) mutant of OsRac1; Acts as a negative control for small GTPases activation assaysDepositorInsertOsRac1(T24N) (LOC4325879 Oryza sativa)
UseTagsCFP-lipid and Venus-CRIBExpressionPlantMutationOsRac1 T24NPromoterUbiAvailable sinceJan. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-INTS3-C
Plasmid#128642PurposeExpress INTS3 C-termini (514-1042 aa) in E. coli with N-terminal and C-terminal His tagDepositorInsertINTS3 C-termini (INTS3 Human)
UseTagsHis tag on N and C-terminalExpressionBacterialMutationINTS3 C-termini (514–1042 aa)PromoterT7Available sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
Anti-Synaptotagmin-12 [N277/7R]
Plasmid#114482PurposeMammalian Expression Plasmid of anti-Synaptotagmin-12 (Mouse). Derived from hybridoma N277/7.DepositorInsertanti-Synaptotagmin-12 (Mus musculus) recombinant mouse monoclonal antibody (Syt12 Mouse)
UseTagsExpressionMammalianMutationPromoterdual CMVAvailable sinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLAP-BUBR1-3A
Plasmid#114054Purposetransfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purificationDepositorInsertBUBR1-3A (BUB1B Human)
UseTagsYFP-TEV-S- Human LAPExpressionMammalianMutationC2823A and G2826A (siRNA-resistance), BUBR1-S670A…PromoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLAP-BUBR1-3D
Plasmid#114055Purposetransfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purificationDepositorInsertBUBR1-3D (BUB1B Human)
UseTagsYFP-TEV-S- Human LAPExpressionMammalianMutationC2823A and G2826A (siRNA-resistance), BUBR1-S670D…PromoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLAP-MIS12-KARD-3D
Plasmid#114058Purposetransfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purificationDepositorInsertMIS12-KARD-3D (BUB1B Human)
UseTagsYFP-TEV-S- Human LAPExpressionMammalianMutationBUBR1-S670D, S676D and T680DPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLAP-MIS12-KARD-3A
Plasmid#114059Purposetransfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purificationDepositorInsertMIS12-KARD-3A (BUB1B Human)
UseTagsYFP-TEV-S- Human LAPExpressionMammalianMutationBUBR1-S670A, S676A and T680APromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAN19-TIR1-GUS-NOSt
Plasmid#108547PurposeCloning vector including C-terminally GUS-tagged Arabidopsis auxin receptor TIR1 [Wild type] and NOS terminatorDepositorInsertTRANSPORT INHIBITOR RESPONSE 1 fused with GUS and NOS terminator (TIR1 Mustard Weed)
UseCloning vectorTagsGUSExpressionMutationPromoterAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAN19-ccvTIR1-GUS-NOSt
Plasmid#108548PurposeCloning vector including C-terminally GUS-tagged Arabidopsis auxin receptor TIR1 with the F79G mutation [ccvTIR1] and NOS terminatorDepositorInsertTRANSPORT INHIBITOR RESPONSE 1 fused with GUS and NOS terminator (TIR1 Mustard Weed)
UseCloning vectorTagsGUSExpressionMutationChanged F79 to GPromoterAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/ TO myc-cCE-bio (cCE-bio)
Plasmid#82473PurposeExpresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal biotin tagged mRNA capping enzyme, inactive formDepositorInsertMyc-NES-mCE (Wt, NLS deletion)-TEV-Bio (Rngtt Mouse, Synthetic)
UseTagsTEV-Biotin and mycExpressionMammalianMutationPromoterCMVAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lrrc4c-Fc-His
Plasmid#72085PurposeExpresses the extracellular region of the LRRC4C protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertLrrc4c (Lrrc4c Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lrrc4c-AP-His
Plasmid#71959PurposeExpresses the extracellular region of the LRRC4C protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertLrrc4c (Lrrc4c Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-HDAC4*
Plasmid#71402PurposeCAG promoter, mammalian expressionDepositorInsertHDAC4* (HDAC4 Human)
UseTagsExpressionMammalianMutationAfter aa126, insertion leads to reading frame shi…PromoterCAGAvailable sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pWZLblasti-HA-MEK5 CBM-E
Plasmid#53169PurposeExpresses HA tagged human MEK5 L280AH281AM323AQ332A from blasticidin resistance retroviral vectorDepositorInsertMEK5 (MAP2K5 Human)
UseRetroviralTagsHAExpressionMammalianMutationL280A/H281A/M323A/Q332APromoterAvailable sinceSept. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-HA-MEK5 CBM-E
Plasmid#53199PurposeExpresses HA tagged human MEK5 L280A/H281A/M323A/Q332A from puromycin resistance retroviral vectorDepositorInsertMEK5 (MAP2K5 Human)
UseRetroviralTagsHAExpressionMammalianMutationL280A/H281A/M323A/Q332APromoterAvailable sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcdna3 T7 Jip1b(471-707)(C478S, C667S and C699T)
Plasmid#52129Purposea mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.DepositorInsertJnk interacting protien (Jip1b)(471-707)(M1+2+6) (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationdeleted amino acids 1-470 and changed C478S, C667…PromoterAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only