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Plasmid#215308PurposeExpresses sfGFP under induction with the IPTG Marionette Cassette (LacIAM + PTac-sfGFP) with a BBR1 backbone.DepositorInsertsfGFP
UseSynthetic BiologyTagsNoneExpressionBacterialMutationPromoterPTacAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMVV103
Plasmid#215309PurposeExpresses sfGFP under induction with the aTc Marionette Cassette (TetR + PTet-sfGFP) with a BBR1 backbone.DepositorInsertsfGFP
UseSynthetic BiologyTagsNoneExpressionBacterialMutationPromoterPtetAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMVV104
Plasmid#215310PurposeExpresses sfGFP under induction with the arabinose Marionette Cassette (AraCAM + araE + PBAD-sfGFP) with a BBR1 backbone.DepositorInsertsfGFP
UseSynthetic BiologyTagsNoneExpressionBacterialMutationPromoterPAraBADAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-UBC-MCP-mNeonGreen
Plasmid#207581PurposeThe lentiviral plasmids for expression of MCP-NeonGreenDepositorInsertMS2 coat protein mNeonGreen fusion
UseLentiviralTagsmNeonGreenExpressionMutationPromoterUBCAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV_PGK_LTB4R
Plasmid#208087PurposeMammalian expression of the wild-type human LTB4 receptor LTB4R.DepositorInsertLTB4R (LTB4R Human)
UseLentiviralTagsExpressionMammalianMutationnonePromoterPGKAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Pur-dCas9-KRAB-MeCP2_hU6-A10
Plasmid#196079PurposeConstitutive CRISPRi vector conferring puromycin resistance, expressing gRNA targeting mouse Adam10.DepositorInsertgRNA targeting Adam10
UseCRISPRTagsExpressionMammalianMutationCloned using SapI sites - destroyed after insertiā¦PromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-A10
Plasmid#196085PurposeInducible CRISPRi vector conferring puromycin resistance, expressing gRNA targeting mouse Adam10.DepositorInsertgRNA targeting Adam10
UseCRISPRTagsExpressionMammalianMutationCloned using SapI sites - destroyed after insertiā¦PromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
UAS-dsarm(E)
Plasmid#187870PurposeGal4/UAS expression of dSarm(isoE)DepositorInsertdSarm isoform E (Sarm Fly)
UseTagsExpressionMutationPromoterAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
ADAM10_Halo_C_allele
Plasmid#178151PurposeDonor vector for endogenous tagging of human ADAM10 at the C-terminus with halotagInsertHalotag (ADAM10 Human)
UseDonor vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-f1(-)-GAPDH-IRES-EGFP
Plasmid#115918Purposedonor to insert IRES-EGFP at the GAPDH locus in human cellsDepositorInsertIRES-EGFP
UseDonor templateTagsdTomatoExpressionMutationPromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only