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Munc18cF119E-pDEST27
Plasmid#85625PurposeGST tagged Munc18c with a F119E mutationDepositorInsertMunc18c (Stxbp3 Mouse)
UseTagsGSTExpressionMammalianMutationF119EPromoterAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
p3E-mCherry-FRB-HA no-pA
Plasmid#82595Purpose3' entry vector containing HA epitope fused to C-terminus and mCherry fused to N-terminus of FRB without pA for rapamycin/rapalog induced dimerizationDepositorInsertmCherry-FRB-HA (no pA)
UseTagsExpressionMutationPromoterNoneAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
LEPG_shSik3.3974
Plasmid#125855PurposeRetroviral expression of mouse Sik3 shRNA with GFP and puromycin resistance geneDepositorInsertshRNA targeting Sik3
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterMSCV-LTRAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR - C3orf17 sgRNA 1
Plasmid#70652PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C3orf17 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.DepositorInsertsgRNA against C3orf17
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
FLAG-ADA add back mutant pCMV5
Plasmid#17554DepositorInsertFLAG-FoxO1-ADA
UseTagsFLAGExpressionMammalianMutationsiRNA-resistant Foxo1 by replacing 3 residues (un…PromoterAvailable sinceOct. 17, 2008AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR - C9orf114 sgRNA 1
Plasmid#70655PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C9orf114 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.DepositorInsertsgRNA against C9orf114
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR - C9orf114 sgRNA 2
Plasmid#70654PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C9orf114 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.DepositorInsertsgRNA against C9orf114
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGDP3 aph(9)-Ia
Plasmid#112882PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertaph(9)-Ia
UseTags6xHisExpressionBacterialMutationPromoterPblaAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmEos2-Linker_PCNA-MutC
Plasmid#98272Purposefor low level expression of mEos2-PCNA in mammalian cellsDepositorInsertPCNA (PCNA Human)
UseTagsmEos2ExpressionMammalianMutationSilent mutated Sequence: GACGCCGTAGTTATATCTTGC (O…PromoterCMVAvailable sinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-TAL-NC2
Plasmid#43855DepositorInsertTALEN-FokI nuclease backbone
UseTagsFlag and FokI nucleaseExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-TAL-NC2
Plasmid#43856DepositorInsertTALEN-FokI nuclease backbone
UseTagsFlag and FokI nucleaseExpressionMammalianMutationPromoterCAGAvailable sinceMarch 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-shNup93-SpeI
Plasmid#87331PurposeExpression of shRNA targeting at Nup93DepositorInsertshRNA against human Nup93
UseRNAiTagsExpressionMammalianMutationPromoterH1Available sinceJune 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCRRNA-G20-R0
Plasmid#101807PurposeRepress mCherry and GFP independently, with different strengthsDepositorInsertcrRNAs targeting GFP and mCherry
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterSpy 1049Available sinceOct. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8-TEV-PseH
Plasmid#89725PurposeExpresses PseH from C. jejuni with an N-terminal 2xHis Gln 5x His feature, followed by the TEV cleavage siteDepositorInsertPseH (pseH Campylobacter jejuni)
UseTags2xHis Gln 5xHis and TEVExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSY091
Plasmid#110068PurposeBacterial expression of L4DepositorInsertL4
UseTagsExpressionMutationAT-swapped mutantPromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSY075
Plasmid#110067PurposeBacterial expression of L2DepositorInsertL2
UseTagsExpressionBacterialMutationAT-swapped mutantPromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNP1 AND gate sfGFP
Plasmid#107361PurposeExpresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 AND input A and pNP1 AND input B are co-expressedDepositorInsertAND gate + sfGFP
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHYDuet93
Plasmid#42303DepositorInsertNpuDnaEdC6
UseTagsH6-GB1ExpressionBacterialMutationPromoterT7Available sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRKVSV Rac T17N
Plasmid#50537DepositorInsertsmall GTPase, mutated (RAC1 Human)
UseTags2X VSVExpressionMammalianMutationT17N (dominant negative)PromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only
